Rapid biosynthesis of irregular shaped gold nanoparticles from macerated aqueous extracellular dried clove buds (Syzygium aromaticum) solution

Deshpande, Raghunandan; Bedre, Mahesh D.; Basavaraja, S.; Sawle, Balaji D.; Manjunath, S. Y.; Venkataraman, A.

doi:10.1016/j.colsurfb.2010.04.003

Cited by 211 publications

(75 citation statements)

References 31 publications

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“…The solution turned pale yellow after 2 min, and the colour change confirmed the presence of AgNPs. The organic moiety of soluble leaf extract is assumed to reduce silver nitrate, thus acting as both stabilizing and reducing agent [43]. The Plasmon peak observed at 435 and 440 nm confirmed the presence of AgNPs (Figures 1(a) and 1(b)).…”

Section: Resultsmentioning

confidence: 75%

Modulatory Effect of Citrate Reduced Gold and Biosynthesized Silver Nanoparticles on α-Amylase Activity

Saware

Aurade

Jayanthi

et al. 2015

Journal of Nanoparticles

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Amylase is one of the important digestive enzymes involved in hydrolysis of starch. In this paper, we describe a novel approach to study the interaction of amylase enzyme with gold nanoparticles (AuNPs) and silver nanoparticles (AgNPs) and checked its catalytic function. AuNPs are synthesized using citrate reduction method and AgNPs were synthesized using biological route employing Ficus benghalensis and Ficus religiosa leaf extract as a reducing and stabilizing agent to reduce silver nitrate to silver atoms. A modulatory effect of nanoparticles on amylase activity was observed. Gold nanoparticles are excellent biocompatible surfaces for the immobilization of enzymes. Immobilized amylase showed 1-to 2-fold increase of activity compared to free enzyme. The biocatalytic activity of amylase in the bioconjugate was marginally enhanced relative to the free enzyme in solution. The bioconjugate material also showed significantly enhanced pH and temperature stability. The results indicate that the present study paves way for the modulator degradation of starch by the enzyme with AuNPs and biogenic AgNPs, which is a promising application in the medical and food industry.

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Section: Resultsmentioning

confidence: 75%

Modulatory Effect of Citrate Reduced Gold and Biosynthesized Silver Nanoparticles on α-Amylase Activity

Saware

Aurade

Jayanthi

et al. 2015

Journal of Nanoparticles

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“…13 Figure 3(a) shows. Clove (Syzygium aromaticum) extract is after reaction with gold ions, this absorption band shows shift towards higher wave number with the loss in intensity [16] [17]. This observation indicates that eugenol molecules may be involved in the reduction of the surface of gold ion.…”

Section: Chemical Functional Group Determinate By Ftirmentioning

confidence: 96%

“…The zeta-potential or net surface charge, of the Clove -AuNPs was also measured. The zeta-potential to be positive, with a value of 2.3 ± 5 mV in (Figure 3(c)) [16] [17] [38]. The neutral colve extract effectively coats the positively charged surface of the AuNPs, but the particles retain their positively charged characteristic.…”

Section: Xrdmentioning

confidence: 99%

Green Synthesis and Characterization of Gold Nanoparticles: Study of Its Biological Mechanism in Human SUDHL-4 Cell Line

Parida¹,

Biswal²,

Bindhani³

2014

ABC

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In this investigation, the anticancer potentiality and biological mechanism of gold nanoparticles (AuNPs) was studied in SUDHL-4 cell line. Metallic AuNPs were prepared and stabilized with ethanol clove (Syzygium aromaticum) extract. The green synthesis of AuNPs was characterized and evaluated by UV-Visible Spectroscopic, X-ray Diffraction (XRD), Fourier Transform Infrared Spectroscopy (FTIR), Transmission electron microscopy (TEM), Dynamic Light Scattering (DLS) and biological activities using various biochemical assays. Green synthesis of AuNPs was confirmed by instrument method. The TEM images show polydisperse, mostly spherical AuNPs particles of 12 -20 nm. AuNPs were decreased the growth and viability of SU-DHL-4 cell line and increased the apoptosis. The treatments of SU-DHL-4 cells with AuNPs resulted in a moderate considerably increase in Reactive oxygen species (ROS) production. We measured apoptosis by Annexin-V/propidium iodide (PI) in the existence and nonexistence of the antioxidant N-acetyl-Lcysteine (NAC), the glutathione-depleting agent buthionine sulfoximine (BSO), or caspase inhibitors to determine the mechanism of cell death. AuNPs are unique potential anticancer agents that cause ROS-dependent apoptosis in SUDHL-4 cell line which was improved by depletion of glutathione (GHS) and inhibited by N-acetyl-L-cysteine on Z-VAD-FMK.

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“…Biosynthesis of nanoparticles has received great attention recently as the synthesized nanoparticles are non-toxic and can be used for biomedical applications. Different fungi like Fusarium oxysporum [10], Cladosporium cladosporioides [11], Fusarium semitactum [12], plants like Syzygium aromaticum [13], Medicago sativa [14], Azadirachta indica [15], Terminalia cuneata [16], and Trignellafoenum graecum seeds [17] have been employed to synthesize metal nanoparticles. Recently, sunlight-induced rapid synthesis of silver nanoparticles has been reported by our group [18,19].…”

Section: Introductionmentioning

confidence: 99%

Antioxidant, antimicrobial and cytotoxic activities of silver and gold nanoparticles synthesized using Plumbago zeylanica bark

2016

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Utilization of bioresources for the synthesis of metal nanoparticles is the latest field in green chemistry. The present work reports the utilization of the aqueous bark extract of Plumbago zeylanica for the biosynthesis of Ag and Au NPs. The Ag and Au NPs thus obtained were characterized by UV-Vis, FT-IR, TEM, XRD, and EDAX analysis. The water-soluble components of the extract were responsible for the reduction of Ag ? and Au 3? ions. FT-IR spectra revealed that the -OH and [C=O groups present in the biomolecules were responsible for reduction and stabilization of nanoparticles. TEM images showed the existence of spherical Ag and Au NPs with average size of 28.47 and 16.89 nm, respectively, which was further substantiated by XRD analysis. The presence of elemental Ag and Au along with C and O from the attached biomolecules was proved by EDAX analysis. The antimicrobial, antioxidant, and in vitro cytotoxic activities of the synthesized nanoparticles were studied by disc diffusion, DPPH, and MTT assay methods, respectively. The free radical inhibition was found to be 78.17 and 87.34 % for Ag and Au nanoparticles, respectively. The Ag and Au NPs showed 61.56 and 65.61 % toxicity against DLA cell line, respectively. The DNA binding ability of Ag and Au NPs were investigated using CT-DNA. The hyperchromism shift inferred the groove binding of nanoparticles with CT-DNA.

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Rapid biosynthesis of irregular shaped gold nanoparticles from macerated aqueous extracellular dried clove buds (Syzygium aromaticum) solution

Cited by 211 publications

References 31 publications

Modulatory Effect of Citrate Reduced Gold and Biosynthesized Silver Nanoparticles on α-Amylase Activity

Modulatory Effect of Citrate Reduced Gold and Biosynthesized Silver Nanoparticles on α-Amylase Activity

Green Synthesis and Characterization of Gold Nanoparticles: Study of Its Biological Mechanism in Human SUDHL-4 Cell Line

Antioxidant, antimicrobial and cytotoxic activities of silver and gold nanoparticles synthesized using Plumbago zeylanica bark

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