Rapid carbohydrate fermentation test for confirmation of the pathogenic Neisseria using a Ba(OH)2 indicator

Slifkin, Malcolm; Pouchet, Gail R.

doi:10.1128/jcm.5.1.15-19.1977

Cited by 10 publications

References 12 publications

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Immunohistochemical localization of a choriogonadotropin-like protein in bacteria isolated from cancer patients

Acevedo

Slifkin

Pouchet

et al. 1978

Cancer

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By the use of specific antibody to human chorionic gonadotropin (CG) as well as to its β‐subunit, and the application of the indirect fluorescein‐labeled and peroxidase‐labeled antibody techniques, we have demonstrated the presence of a membrane (wall)‐associated CG‐similar immunoreactive protein in 15 strains of bacteria isolated from tissues of patients bearing malignant neoplasms. These microorganisms were classified as S. epidermidis, (12), E. coli (2), and a single strain of P. maltophilia (ATCC 13637). The absence of the CG‐like antigen in other “cancer associated bacteria”, Streptococcus faecalis (ATCC 12818) and Pseudomonas aeruginosa (from patient with cancer of colon), demonstrated that not every “cancer associated bacteria” has the capability to synthesize the trophoblastic‐like protein. The negative results obtained with a number of “noncancer control” bacteria of known origin, obtained from ATCC and from clinical samples, strongly supported the idea that the existence of these CG‐like protein producing microorganisms is not a ubiquitous finding. The demonstration of a de novo bacterial biosynthesis of a protein having similar antigenic and biophysical properties to those of the human trophoblastic hormone, has great biological implications, especially if its biosynthesis is proven only in bacterial strains growing in the presence of cancer cells in which we have already demonstrated the presence of a similar antigen. The explanation of the phenomenon is unknown. Because of their origin, the potential of “genetic exchange” with subsequent expression of the mammalian gene by the bacterial cells becomes a possibility. It is also possible that the gene coding for the CG‐like protein is normally present but inactive or repressed in all bacteria.

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Immunohistochemical localization of a choriogonadotropin-like protein in bacteria isolated from cancer patients

Acevedo

Slifkin

Pouchet

et al. 1978

Cancer

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Standard Microbiological Techniques (Staining, Morphological and Cultural Characteristics, Biochemical Properties, and Serotyping) in the Detection of ESKAPE Pathogens

Parasuraman,

Busi,

Lee

2024

ESKAPE Pathogens

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Comparative use of amino acids by three auxotypes of Neisseria gonorrhoeae

Pillon

Chan

Franczyk

et al. 1988

Antonie van Leeuwenhoek

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Auxotypes of Neisseria gonorrhoeae are usually distinguishable by their particular requirements for growth; these requirements often include amino acids. It is possible that strains needing particular substrates to grow can be distinguished not merely by their growth requirements but also by their metabolism of these particular substrates. In this work amino acid utilization and oxidation studies were performed enabling prototype, pro- and thia-strains to be distinguished. The metabolism study also underlined the importance of proline as an energy source and pointed to the probability of distinct relationships with the metabolism of the key amino acids, glutamic and aspartic acids, for the three auxotypes. It is proposed that the specific amino acid required by the naturally occurring auxotype serves as an energy source at the site of infection and has important implications with respect to particular auxotypes at various sites.

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Rapid carbohydrate fermentation test for confirmation of the pathogenic Neisseria using a Ba(OH)2 indicator

Cited by 10 publications

References 12 publications

Immunohistochemical localization of a choriogonadotropin-like protein in bacteria isolated from cancer patients

Immunohistochemical localization of a choriogonadotropin-like protein in bacteria isolated from cancer patients

Standard Microbiological Techniques (Staining, Morphological and Cultural Characteristics, Biochemical Properties, and Serotyping) in the Detection of ESKAPE Pathogens

Comparative use of amino acids by three auxotypes of Neisseria gonorrhoeae

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