Rapid changes in liver lipid composition and pancreatic islet K+ handling and secretory behaviour provoked by the intravenous administration of a medium-chain triglyceride:fish oil emulsion to long-chain polyunsaturated ω3 fatty acid-depleted rats

Abstract. Second-generation rats depleted in long-chain polyunsaturated ˆ3 fatty acids were recently proposed as a novel animal model for the metabolic syndrome. In the present study, a dietary deprivation of ˆ3 acids for 3-7 months was found sufficient to provoke in 6-week-old normal rats the same alteration of the fatty acid content and profile of liver phospholipids and triglycerides as that otherwise prevailing in the second-generation ˆ3-depleted rats, with emphasis on a severe decrease in their ˆ3 fatty acid content, alterations in the relative contribution of and ratio between selected long-chain polyunsaturated ˆ6 fatty acids, saturated and monodesaturated fatty acids and precursors of nervonic acid, and liver steatosis. When the ˆ3-depleted rats were exposed, after the first 7 months of the present experiments and for 2-4 weeks to a diet supplemented with 5% (w/w) flaxseed oil, most of these hepatic variables returned towards or beyond control values. In both the ˆ3-depleted rats and control animals, however, the eventual exposure to the flaxseed oilenriched diet failed to suppress liver steatosis and, on the contrary, provoked a further increase in liver triglyceride content. It is proposed, therefore, that the present approach represents a simple and realistic animal model to study the consequences of ˆ3-depletion. Moreover, the results suggest that to oppose such consequences, e.g. liver steatosis, it may be necessary to combine the dietary supply of ˆ3 acids with a suitable control of food intake, in both qualitative and quantitative terms.

Section: Discussionmentioning

confidence: 62%

The metabolic syndrome of ω3-depleted rats. I. Liver data

Malaisse

Bulur²,

Zhang³

et al. 2009

“…Second, in order to further assess the significance of changes in the total fatty acid content of triglycerides, advantage was taken of the positive correlation between the C16:1ω7 weight percentage of liver triglycerides and their total fatty acid content, as herein documented in the uninjected ω3-depleted rats and those injected with the control MCT:OO emulsion. Likewise, in a larger series of 18 uninjected ω3-depleted female rats, there was a significant positive correlation (r=0.541; p<0.05) between the C16:1ω7 weight percentage of liver triglycerides and their total fatty acid content (3).…”

Section: Discussionmentioning

confidence: 74%

Rapid lipid enrichment in ω3 fatty acids: Liver data

Carpentier¹,

Peltier²,

Portois³

et al. 2008

Self Cite

“…First, male and female rats were examined in the course of the same series of experiments, all injected animals being examined 60 min after the intravenous administration of the lipid emulsion. In prior investigations, male and female rats were examined in separate laboratories under distinct experimental conditions, the male rats being also much older than the female animals (7)(8)(9). Moreover, the latter animals were sacrified either 60 or 120 min after intravenous injection of the lipid emulsion.…”

Section: Discussionmentioning

confidence: 99%

Rapid lipid enrichment in ω3 fatty acids: Plasma data

Carpentier¹,

Peltier²,

Portois³

et al. 2008

Self Cite

Abstract. The bolus intravenous injection of a novel mediumchain triglyceride:fish oil emulsion to normal subjects was recently reported to enrich within 60 min the phospholipid content of leucocytes and platelets in long-chain polyunsaturated ω3 fatty acids. The present study, conducted in second generation ω3-depleted rats, aimed at investigating whether such a procedure may also increase within 60 min the phospholipid content of ω3 fatty acids in cells located outwards the bloodstream, in this case liver cells, and whether this coincides with correction of the perturbation in the liver triglyceride fatty acid content and profile otherwise prevailing in these rats. This first report deals mainly with the fatty acid pattern of plasma lipids in male ω3-depleted rats that were non-injected or injected with either the ω3-rich emulsion or a control medium-chain triglyceride:olive oil emulsion. The results provide information on the fate of the exogenous lipids present in the lipid emulsions and injected intravenously 60 min before sacrifice. Moreover, in the uninjected ω3-depleted rats the comparison between individual plasma and liver measurements indicated positive correlations in the fatty acid profile of phospholipids and triglycerides.