Rapid Characterization of Glycosaminoglycans Using a Combined Approach by Infrared and Raman Microspectroscopies

Mainreck, Nathalie; Brézillon, Stéphane; Sockalingum, Ganesh D.; Maquart, François‐Xavier; Manfait, Michel; Wegrowski, Yanusz

doi:10.1002/jps.22288

Cited by 62 publications

(80 citation statements)

References 32 publications

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“…Chondroitin sulphate (CS) is a sulphated glycosaminoglycan (GAG) consisting of a long unbranched polysaccharide chain with a repeating disaccharide structure of N‐acetylgalactosamine and glucuronic acid. As a major component of the extracellular matrix of numerous connective tissues, such as cartilage, bone, skin, ligaments, and tendons, it is responsible for many important biomechanical properties such as resistance, elasticity, tissue stiffness, and resilience . The consistency given to tissues by sulphated GAGs is due to their high capacity to retain interstitial water conferred by sulphate groups, which promote the formation of large negatively‐charged molecules that attract cations and water molecules, leading to the obtaining of a hydrated gel .…”

Section: Introductionmentioning

confidence: 99%

“…As a major component of the extracellular matrix of numerous connective tissues, such as cartilage, bone, skin, ligaments, and tendons, it is responsible for many important biomechanical properties such as resistance, elasticity, tissue stiffness, and resilience . The consistency given to tissues by sulphated GAGs is due to their high capacity to retain interstitial water conferred by sulphate groups, which promote the formation of large negatively‐charged molecules that attract cations and water molecules, leading to the obtaining of a hydrated gel . Sulphated GAGs simultaneously mediate in many other biological processes ranging from signalling pathways of cell differentiation, anticoagulation, and growth pathways to cartilage's ability to sustain stress during compression.…”

Section: Introductionmentioning

confidence: 99%

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Quantitative evaluation of sulfation position prevalence in chondroitin sulphate by Raman spectroscopy

López‐Álvarez

López-Senra

Valcárcel

et al. 2019

J Raman Spectroscopy

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Chondroitin sulphate (CS) as a major component of the extracellular matrix of numerous connective tissues is responsible for biomechanical properties such as resistance and elasticity. It occurs in different isomeric forms with different sites and degrees of sulfation. The characterization of crude CS and quantitative isomeric identification by high‐performance liquid chromatography (HPLC) requires specific enzymes, expensive reagents, and a complicated analytical process. Poor reproducibility and imprecise quantification were found with other methodologies. Raman spectroscopy allows rapid and reproducible identification of CS isomers offering specificity and avoids the need for sample pretreatment or external markers. In the present work, a quantitative identification of major contribution of isomeric chondroitin 4 and 6‐sulphate in crude CS by Raman spectroscopy has been performed. Two quantitative indices have been proposed based on areas of specific bands of interest related to main skeletal modes, an aromatic ring with axial orientation in the case of the major contribution of 4‐sulphate and C‐O‐(S) vibration, which is heavily detected in the case of the major contribution of 6‐sulphate. Both mammalian and fish sources of CS were used to validate the ability of mentioned indices to discriminate between prevalence of 4‐sulphate, 6‐sulphate, or none of them. Correspondence of given results with HPLC was demonstrated.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Quantitative evaluation of sulfation position prevalence in chondroitin sulphate by Raman spectroscopy

López‐Álvarez

López-Senra

Valcárcel

et al. 2019

J Raman Spectroscopy

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“…We previously showed that VS such as IR and Raman microspectroscopies are promising techniques to characterize, differentiate, and classify GAGs types and subtypes despite their close molecular structures [52]. Some characteristic spectral regions and bands were identified and used in combination with chemometric methods such as HCA and PCA, to characterize standard GAGs by VS [52, 53]. These approaches could be extremely useful for performing quality tests on GAG samples.…”

Section: Infrared and Raman Microspectroscopies: Rapid And Non-invasimentioning

confidence: 99%

“…Spectroscopic markers characteristic of reference GAG molecules have been identified previously based on their molecular structures by Raman and infrared (IR) microspectroscopies [52, 53]. These techniques are sensitive enough to descriminate different cell types according to their capacity to synthetise GAGs [54].…”

Section: Introductionmentioning

confidence: 99%

Implementation of infrared and Raman modalities for glycosaminoglycan characterization in complex systems

et al. 2016

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Glycosaminoglycans (GAGs) are natural, linear and negatively charged heteropolysaccharides which are incident in every mammalian tissue. They consist of repeating disaccharide units, which are composed of either sulfated or non-sulfated monosaccharides. Depending on tissue types, GAGs exhibit structural heterogeneity such as the position and degree of sulfation or within their disaccharide units composition being heparin, heparan sulfate, chondroitine sulfate, dermatan sulfate, keratan sulfate, and hyaluronic acid. They are covalently linked to a core protein (proteoglycans) or as free chains (hyaluronan). GAGs affect cell properties and functions either by direct interaction with cell receptors or by sequestration of growth factors. These evidences of divert biological roles of GAGs make their characterization at cell and tissue levels of importance. Thus, non-invasive techniques are interesting to investigate, to qualitatively and quantitatively characterize GAGs in vitro in order to use them as diagnostic biomarkers and/or as therapeutic targets in several human diseases including cancer. Infrared and Raman microspectroscopies and imaging are sensitive enough to differentiate and classify GAG types and subtypes in spite of their close molecular structures. Spectroscopic markers characteristic of reference GAG molecules were identified. Beyond these investigations of the standard GAG spectral signature, infrared and Raman spectral signatures of GAG were searched in complex biological systems like cells. The aim of the present review is to describe the implementation of these complementary vibrational spectroscopy techniques, and to discuss their potentials, advantages and disadvantages for GAG analysis. In addition, this review presents new data as we show for the first time GAG infrared and Raman spectral signatures from conditioned media and live cells, respectively.

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“…FTIR spectroscopy is a non-destructive, label-free method for studying molecular composition and the structure of macromolecules, either in their isolated form 9 or within complex biological systems such as cells and tissues 10 . It can quantitatively or qualitatively analyse spectral variations within the sample that may be linked to various molecular constituents, such as nucleic acids, carbohydrates proteins or lipids 11 .…”

Section: Introductionmentioning

confidence: 99%

Development of a high throughput (HT) Raman spectroscopy method for rapid screening of liquid blood plasma from prostate cancer patients

Medipally¹,

Maguire²,

Bryant

et al. 2017

Analyst

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Rapid Characterization of Glycosaminoglycans Using a Combined Approach by Infrared and Raman Microspectroscopies

Cited by 62 publications

References 32 publications

Quantitative evaluation of sulfation position prevalence in chondroitin sulphate by Raman spectroscopy

Quantitative evaluation of sulfation position prevalence in chondroitin sulphate by Raman spectroscopy

Implementation of infrared and Raman modalities for glycosaminoglycan characterization in complex systems

Development of a high throughput (HT) Raman spectroscopy method for rapid screening of liquid blood plasma from prostate cancer patients

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