Rapid chemotaxonomic profiling for the identification of high-value carotenoids in microalgae

Gee, Dónal Mc; Archer, Lorraine; Paskuliakova, Andrea; Coy, Gary R. Mc; Fleming, Gerard T.A.; Gillespie, Eoin; Touzet, Nicolas

doi:10.1007/s10811-017-1247-7

Cited by 31 publications

(15 citation statements)

References 57 publications

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“…By using an untargeted metabolomics approach, we were able to illustrate that microalgae produce diverse suites of metabolites and that there is little evidence that a core metabolome exists that is shared across taxonomic boundaries. This is surprising as various classes of microalgae are distinguished by their carotenoid profiles, as well as morphology and genetic phylogeny [ 41 ]. This is also very exciting as macroalgae have been reported to have biological activities [ 42 , 43 ] that appear, from this study, to be distinct from those produced by their microalgal relations.…”

Section: Discussionmentioning

confidence: 99%

Exploring the Chemical Space of Macro- and Micro-Algae Using Comparative Metabolomics

et al. 2021

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With more than 156,000 described species, eukaryotic algae (both macro- and micro-algae) are a rich source of biological diversity, however their chemical diversity remains largely unexplored. Specialised metabolites with promising biological activities have been widely reported for seaweeds, and more recently extracts from microalgae have exhibited activity in anticancer, antimicrobial, and antioxidant screens. However, we are still missing critical information on the distinction of chemical profiles between macro- and microalgae, as well as the chemical space these metabolites cover. This study has used an untargeted comparative metabolomics approach to explore the chemical diversity of seven seaweeds and 36 microalgal strains. A total of 1390 liquid chromatography-mass spectrometry (LC-MS) features were detected, representing small organic algal metabolites, with no overlap between the seaweeds and microalgae. An in-depth analysis of four Dunaliella tertiolecta strains shows that environmental factors may play a larger role than phylogeny when classifying their metabolomic profiles.

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Section: Discussionmentioning

confidence: 99%

Exploring the Chemical Space of Macro- and Micro-Algae Using Comparative Metabolomics

et al. 2021

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“…Pigment extraction and analysis Pigment extraction was carried out according to Mc Gee et al (2018). Samples of freeze-dried biomass (2-3 mg) were mixed with 500 μl of ice cold 100% acetone and glass beads and placed in a FastPrep FP120 ribolyser for 40 s at full speed.…”

Section: 11mentioning

confidence: 99%

Nanoalgosomes: Introducing extracellular vesicles produced by microalgae

Adamo

Fierli

Romancino

et al. 2021

J of Extracellular Vesicle

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126

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Cellular, inter‐organismal and cross kingdom communication via extracellular vesicles (EVs) is intensively studied in basic science with high expectation for a large variety of bio‐technological applications. EVs intrinsically possess many attributes of a drug delivery vehicle. Beyond the implications for basic cell biology, academic and industrial interests in EVs have increased in the last few years. Microalgae constitute sustainable and renewable sources of bioactive compounds with a range of sectoral applications, including the formulation of health supplements, cosmetic products and food ingredients. Here we describe a newly discovered subtype of EVs derived from microalgae, which we named nanoalgosomes. We isolated these extracellular nano‐objects from cultures of microalgal strains, including the marine photosynthetic chlorophyte Tetraselmis chuii, using differential ultracentrifugation or tangential flow fractionation and focusing on the nanosized small EVs (sEVs). We explore different biochemical and physical properties and we show that nanoalgosomes are efficiently taken up by mammalian cell lines, confirming the cross kingdom communication potential of EVs. This is the first detailed description of such membranous nanovesicles from microalgae. With respect to EVs isolated from other organisms, nanoalgosomes present several advantages in that microalgae are a renewable and sustainable natural source, which could easily be scalable in terms of nanoalgosome production.

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“…They are widely distributed in nature and more than 250 carotenoids are of marine origin [2]. The different compositions that are present in the marine microorganisms, such as microalgae and phytoplankton, has promoted the use of carotenoids as a chemical signature for rapid chemotaxonomic profiling [3,4,5,6]. In the photosynthesis process, their main functions are light harvesting and photoprotections, e.g., protection against reactive singlet oxygen and protection against blue light radiation [7].…”

Section: Introductionmentioning

confidence: 99%

Sulfur-Containing Carotenoids from A Marine Coral Symbiont Erythrobacter flavus Strain KJ5

et al. 2019

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Erythrobacter flavus strain KJ5 (formerly called Erythrobacter sp. strain KJ5) is a yellowish marine bacterium that was isolated from a hard coral Acropora nasuta in the Karimunjawa Islands, Indonesia. The complete genome sequence of the bacterium has been reported recently. In this study, we examined the carotenoid composition of this bacterium using high-performance liquid chromatography coupled with ESI-MS/MS. We found that the bacterium produced sulfur-containing carotenoids, i.e., caloxanthin sulfate and nostoxanthin sulfate, as the most abundant carotenoids. A new carotenoid zeaxanthin sulfate was detected based on its ESI-MS/MS spectrum. The unique presence of sulfated carotenoids found among the currently known species of the Erythrobacter genus were discussed.

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Rapid chemotaxonomic profiling for the identification of high-value carotenoids in microalgae

Cited by 31 publications

References 57 publications

Exploring the Chemical Space of Macro- and Micro-Algae Using Comparative Metabolomics

Exploring the Chemical Space of Macro- and Micro-Algae Using Comparative Metabolomics

Nanoalgosomes: Introducing extracellular vesicles produced by microalgae

Sulfur-Containing Carotenoids from A Marine Coral Symbiont Erythrobacter flavus Strain KJ5

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