Rapid Colorimetric Detection of Wound Infection with a Fluidic Paper Device

Abstract

Current procedures for the assessment of chronic wound infection are time-consuming and require complex instruments and trained personnel. The incidence of chronic wounds worldwide, and the associated economic burden, urge for simple and cheap point-of-care testing (PoCT) devices for fast on-site diagnosis to enable appropriate early treatment. The enzyme myeloperoxidase (MPO), whose activity in infected wounds is about ten times higher than in non-infected wounds, appears to be a suitable biomarker for wound infection diagnosis. Herein, we develop a single-component foldable paper-based device for the detection of MPO in wound fluids. The analyte detection is achieved in two steps: (i) selective immunocapture of MPO, and (ii) reaction of a specific dye with the captured MPO, yielding a purple color with increasing intensity as a function of the MPO activity in infected wounds in the range of 20–85 U/mL. Ex vivo experiments with wound fluids validated the analytic efficiency of the paper-based device, and the results strongly correlate with a spectrophotometric assay.

Direct metagenomics investigation of non-surgical hard-to-heal wounds: a review

“…Routine molecular detection tools of chronic wounds infections are based on simplex or multiplex real-time PCR targeting a limited number of bacteria commonly isolated from chronic wound samples, as well as the partial sequencing of the 16 S rDNA [23,24]. The lack of universal identification of microorganisms involved in these infections complicates diagnosis and patient management.…”