Rapid colorimetric determination of free fatty acids

Lowry, Robert R.; Tinsley, Ian J.

doi:10.1007/bf02636814

Cited by 629 publications

(300 citation statements)

References 5 publications

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“…Reducing sugars content was determined by following the method of Nelson (1944). The content of phospholipids was estimated by determining phosphorus content as described by Ames (1966), glycolipids by measuring glucose content according to Roughan and Batt (1968), free fatty acids by copper complex formation as described by Lowry and Tinsley (1976) and sterols by using ferric chloride according to Zak (1957), all spectrophotometrically. The content of total glycerides was calculated by the difference of total lipid classes from 100.…”

Section: Methodsmentioning

confidence: 99%

Positional effects on soybean seed composition during storage

et al. 2011

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This investigation was conducted in order to determine the degree of biochemical changes during natural ageing of soybean seeds borne on different positions on stem axis. Biochemical analysis of field grown soybean seeds revealed significant differences in their oil and protein contents as a function of nodal positions. Both oil and protein content decreased after 180 days of storage (DOS) at all the nodal positions. Proportions of membrane lipids in seeds were less in basal as compared to apical positions and their proportion in stored seeds also decreased with increasing storage periods. The content of starch, total soluble sugars and reducing sugars in seeds decreased during storage for 180 days but it didn't show positional variations in their contents. The extent of lipid peroxidation increased during storage was associated with the corresponding decrease in the activities of antioxidant enzymes viz catalase and peroxidase in seeds. Seeds at basal positions showed higher rate of lipid peroxidation and lower peroxidase activity as compared to apical positions. Results suggest that soybean seeds collected from basal positions showed higher deteriorative changes during storage in comparison to apical positions that might be related to higher lipid content in seeds from basal portion of soybean stem axis as compared to apical portion.

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Section: Methodsmentioning

confidence: 99%

Positional effects on soybean seed composition during storage

et al. 2011

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“…After removal of excess solvent, the fat was dried, weighed and analysed for FFA by colorimetric procedure (Lowry and Tinsley 1976). The amount of FFA was calculated as palmitic acid equivalent from the calibration curve using different aliquots of standard palmitic acid and expressed as% on lipid basis.…”

Section: Methodsmentioning

confidence: 99%

Effect of ethanolic extract of clove on the keeping quality of fresh mutton during storage at ambient temperature (25 ± 2 °C)

et al. 2011

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Antimicrobial potential of ethanolic extract of clove (Eugenia caryophyllata) on fresh mutton during storage at 25±2°C was evaluated. The extract inhibited spoilage and pathogenic microflora of mutton previously treated with acidulants to reduce surface microbial load and the surface pH, up to 4 days without any deleterious change in sensory and physical qualities. Biomarker cadaverine, an indicator of spoiling/spoiled mutton, was present in 1 day stored control samples and absent up to 4 days in treated mutton. The levels of other biomarkers like biogenic amine index (0.31 mg/100 g) and free fatty acids (1.52%) were lower in 4 days stored treated samples than 1 day stored control samples (3.6 mg/100 g and 2.4%, respectively). Thus, ethanolic extract of clove can be effectively used to improve the keeping quality of fresh mutton up to 4 days at 25±2°C.

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“…The method is specific for fatty acids and more sensitive in comparison to titrimetric method [29,30]. Several authors …”

Section: Lipase Screening Liquid Mediummentioning

confidence: 99%

Isolation and Identification of a Novel Lipase Producing Bacteria from Oil Spilled Soil

Veerapagu¹,

Narayanan²,

Jeya³

et al. 2014

IJIRSET

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Lipases are glycerol ester hydrolases that catalyze the hydrolysis of triglycerides to free fatty acids and glycerol. Oil spilled soil samples collected from Dharmapuri and Salem districts were screened for a novel lipase producing bacteria by screening in Tributyrin agar plate and Rhodamine olive oil agar and lipase screening liquid medium. Among the two hundred isolates thirty two isolates exhibited high lipolytic activity on TBA plate assay and twenty isolate exhibited high lipolytic activity on ROA plate assay The isolate BLP 141 produces lipase more than 12 U/ml in the further lipase screening liquid medium was selected as a novel lipase producing bacteria and identified by morphological and biochemical characteristics as Pseudomonas. The 16S rRNA gene amplified by bacterial universal primer 27F and 1492R yielded 1478 bp was analysed by BLAST confirmed it as Pseudomonas gessardii and was submitted in GenBank with accession number KJ547711.

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Rapid colorimetric determination of free fatty acids

Cited by 629 publications

References 5 publications

Positional effects on soybean seed composition during storage

Positional effects on soybean seed composition during storage

Effect of ethanolic extract of clove on the keeping quality of fresh mutton during storage at ambient temperature (25 ± 2 °C)

Isolation and Identification of a Novel Lipase Producing Bacteria from Oil Spilled Soil

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