Rapid communication: dinucleotide repeat polymorphism of rainbow trout, FGT5

Sakamoto, Takashi; Okamoto, Nobuaki; Ikeda, Yoshichika

doi:10.2527/1994.72102768x

Cited by 5 publications

(2 citation statements)

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“…disease resistance, we are attempting to iso late DNA markers from the rainbow trout genome. [17][18][19][20][21] In fisheries sciences, many species need to be analyzed. If DNA markers isolated from one species can be used for different species, they must be a more profitable tool for ge nome analysis in fisheries sciences.…”

Section: Introductionmentioning

confidence: 99%

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Application of PCR Primer Pairs from Rainbow Trout to Detect Polymorphisms of CA Repeat DNA Loci in Five Confamilial Species

1996

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Dinucleotide repeat polymorphisms are widely used as markers for genetic analysis in mammalian genomes because of their abundance and high variability among individuals. We have previously isolat ed informative markers for genetic studies of rainbow trout. In fisheries science, many species need to be analyzed. Therefore, heterologous applications of DNA markers designed in one species can be use ful. The sequences flanking a dinucleotide repeat are often conserved among closely related species. We thus wanted to determine whether DNA markers isolated from the rainbow trout genome could be used to detect dinucleotide repeat polymorphisms in five other species of salmonid. Five sets of primer pairs that flank rainbow trout microsatellites and that produced polymorphic PCR products, were tested with template DNA from five species. Specific PCR products were obtained in all cases. Four out of five DNA markers could detect polymorphism in all species and all five DNA markers could detect polymor phism in at least one species. In many cases, these markers showed large numbers of alleles and high heterozygosity values in other salmonids such as rainbow trout. Our results show that DNA markers iso lated from rainbow trout are also useful in other salmonids for fisheries sciences such as population stu dies and genetic linkage analysis.

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Section: Introductionmentioning

confidence: 99%

“…In mammals, the loca tions of dinucleotide microsatellites are often conserved among closely related species, and can serve for detecting polymorphisms in several different species. 11,21) In fisheries science, a trinucleotide microsatellite cloned from the rain bow trout genome was employed in different species for DNA fingerprinting. 23…”

Section: Introductionmentioning

confidence: 99%

Application of PCR Primer Pairs from Rainbow Trout to Detect Polymorphisms of CA Repeat DNA Loci in Five Confamilial Species

1996

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Production of androgenetic amago salmon Oncorhynchus masou ishikawae with dispermy fertilization

2010

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With the aim of improving artificial androgenesis in teleost fishes, we tested two methods for producing androgenetic diploids of amago salmon (Oncorhynchus masou ishikawae), namely, fertilization of gamma-ray irradiated eggs with fused spermatozoa (sperm-fusion method) and the fertilization of irradiated eggs with untreated sperm followed by the blocking of cell division (mitosis-inhibition method). Our results showed that the optimal condition for sperm fusion was to treat the sperm with 50% polyethylene glycol (molecular weight 7500) for 100 s. The efficiency of the two methods of androgenesis was compared in terms of fertilization rate, hatching rate, and larval survival after hatching. The rate of fertilization was lower with the sperm-fusion method than with the mitosis-inhibition method, but the reverse was true for the hatching rate. The survival rate of hatched larvae was the same with the two methods. Androgenesis was confirmed with a recessive albino color marker, and all viable offspring were found to be heterozygous based on analysis of the microsatellite markers. Our results suggest that androgenesis with the sperm-fusion method is a promising approach with potential applications in both aquaculture breeding programs and the preservation of endangered freshwater fishes.

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Relatedness determination in the absence of pedigree information in three cultured strains of rainbow trout (Oncorhynchus mykiss)

McDonald¹,

Danzmann²,

Ferguson³

2004

Aquaculture

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Rapid communication: dinucleotide repeat polymorphism of rainbow trout, FGT5

Cited by 5 publications

References 0 publications

Application of PCR Primer Pairs from Rainbow Trout to Detect Polymorphisms of CA Repeat DNA Loci in Five Confamilial Species

Application of PCR Primer Pairs from Rainbow Trout to Detect Polymorphisms of CA Repeat DNA Loci in Five Confamilial Species

Production of androgenetic amago salmon Oncorhynchus masou ishikawae with dispermy fertilization

Relatedness determination in the absence of pedigree information in three cultured strains of rainbow trout (Oncorhynchus mykiss)

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