Rapid confirmatory identification of Neisseria gonorrhoeae with lectins and chromogenic substrates

Yajko, D M; Chu, Adrian; Hadley, W K

doi:10.1128/jcm.19.3.380-382.1984

Cited by 26 publications

(7 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…), Philip and Garton (4) found that 1 of 22 strains of N. meningitidis that they tested was negative for GAP, while Janda et al (3) found 5 of 172 N. meningitidis isolates negative for GAP. PAP-positive N. meningitidis isolates have also been reported by Yajko et al (7), who found 3 of 7 isolates positive, and by D'Amato et al…”

supporting

confidence: 54%

Increased detection of prolylaminopeptidase in Neisseria meningitidis by Identicult-Neisseria

Sperry¹,

Cohenford²,

Campognone³

et al. 1986

J Clin Microbiol

View full text Add to dashboard Cite

a rapid enzymatic method with chromogenic substrates, was tested in our laboratories for the identification of Neisseria gonorrhoeae, Neisseria meningitidis, and Neisseria lactamica. The test correlated very highly in its identification of pathogenic Neisseria spp. with modified New York City fermentation medium. Identicult-Neisseria appeared to be more sensitive in its detection of prolylaminopeptidase activity in N. meningitidis than most of the currently available systems.

show abstract

supporting

confidence: 54%

Increased detection of prolylaminopeptidase in Neisseria meningitidis by Identicult-Neisseria

Sperry¹,

Cohenford²,

Campognone³

et al. 1986

J Clin Microbiol

View full text Add to dashboard Cite

show abstract

“…The Gonochek Il test (Du Pont Co., Wilmington, Del. ), which has also been described previously, is based on the differential utilization of three chromogenic substrates (5-bromo-4-chloro-3-indoyl-3-D-galactopyranoside, -y-glutamyl-para-nitroanilide, 3-niapthyl amino acid derivative) by Neisseria species (6,14,21,25,26). The microbial identification tubes were supplied with the kit.…”

Section: Minitek the Minitek Kit (Miniaturized Microorganismmentioning

confidence: 99%

Evaluation of eight methods for identification of pathogenic Neisseria species: Neisseria-Kwik, RIM-N, Gonobio-Test, Minitek, Gonochek II, GonoGen, Phadebact Monoclonal GC OMNI Test, and Syva MicroTrak Test

1988

View full text Add to dashboard Cite

The performance of eight methods in identifying Neisseria species, particularly N. gonorrhoeae, was evaluated. These methods included four rapid carbohydrate utilization tests (Gonobio-Test, Neisseria-Kwik, RIM-N, and Minitek); the Gonochek 11, a test which is based on the utilization of chromogenic substrates; and three monoclonal antibody tests (Syva MicroTrak, GonoGen, and Phadebact Monoclonal GC OMNI Test). In all, 182 isolates comprised in six species of Neisseria as well as Branhamella catarrhalis and Moraxella sp. were tested. Cystine-tryptic digest agar supplemented with sugars was included for reference purposes. In the carbohydrate utilization tests, the sensitivity and specificity of the Neisseria-Kwik and Minitek tests for the identification of N. gonorrhoeae were 100%. This compared with sensitivities and specificities, respectively, of 100 and 99.1% for the Gonobio-Test and 99.1 and 100% for cystine-tryptic digest agar sugars and the RIM-N test. The sensitivity and specificity of the Gonochek II test were 99.0 and 86.7%, respectively. Although most test kits did not claim to identify all Neisseria species, in several cases isolates of N. subflava were misidentified or could be misinterpreted as N. gonorrhoeae or N. meningitidis. With the monoclonal reagents, the Syva MicroTrak system was 100% sensitive and 100% specific. The GonoGen test was both 99.1% sensitive and specific, while the Phadebact Monoclonal GC OMNI Test was 99.1% sensitive but 91.2% specific. With this latter test, cross-reactions were observed with strains of B. catarrhalis, N. cinerea, and N. lactamica.

show abstract

“…Rapid identification of Neisseria gonorrhoeae may be accomplished by several diverse methods. The methods include carbohydrate utilization with preformed enzymes or enzymes formed during growth (5,8,12,15,20) and immunological (4,7,10,13), chromogenic (2,19), and radiometric (16) procedures. Immunological procedures include coagglutination with polyclonal (11) (Phadebact Gonococcus Test; Pharmacia Diagnostics, Piscataway, N.J.) and murine monoclonal (9) (Gono Gen; New Horizons Diagnostics, Columbia, Md.)…”

mentioning

confidence: 99%

Fluorescent monoclonal antibody compared with carbohydrate utilization for rapid identification of Neisseria gonorrhoeae

Welch

Cartwright

1988

J Clin Microbiol

View full text Add to dashboard Cite

A commercially available fluorescein-conjugated monoclonal antibody (MAb) (Syva Co., Palo Alto, Calif.; Genetic Systems, Seattle, Wash.) against Neisseria gonorrhoeae was compared with a standard cystine Trypticase agar (CTA) sugar utilization method and with three rapid carbohydrate utilization tests, including the Minitek (BBL Microbiology Systems, Cockeysville, Md.), Neisseria-Stat (Richardson Scientific, Dallas, Tex.), and Neisseria-Kwik (Micro-Biologics, St. Cloud, Minn.) systems for the identification of Neisseria species. The MAb correctly identified ail 86 clinical isolates of N. gonorrhoeae. Of these 86 isolates, 28 were found later (48 h after the initial inoculation) to be contaminated with non-Neisseria bacteria. In the other four test systems studied, the identification rates for pure and contaminated N. gonorrhoeae cultures were, respectively, as follows: CTA sugars, 88 and 32%; Minitek, 67 and 50%; Neisseria-Stat, 97 and 96%; and Neisseria-Kwik, 80 and 74%. The MAb did not identify any of the 50 nongonoccocal Neisseria isolates tested. The most expensive test system was the MAb, followed by the Neisseria-Kwik, Minitek, Neisseria-Stat, and CTA sugars systems. The MAb appears to be a rapid and accurate method to identify in vitro isolates of N. gonorrhoeae.

show abstract

Rapid confirmatory identification of Neisseria gonorrhoeae with lectins and chromogenic substrates

Cited by 26 publications

References 13 publications

Increased detection of prolylaminopeptidase in Neisseria meningitidis by Identicult-Neisseria

Increased detection of prolylaminopeptidase in Neisseria meningitidis by Identicult-Neisseria

Evaluation of eight methods for identification of pathogenic Neisseria species: Neisseria-Kwik, RIM-N, Gonobio-Test, Minitek, Gonochek II, GonoGen, Phadebact Monoclonal GC OMNI Test, and Syva MicroTrak Test

Fluorescent monoclonal antibody compared with carbohydrate utilization for rapid identification of Neisseria gonorrhoeae

Contact Info

Product

Resources

About