Rapid Construction of Three‐Dimensional Multilayered Tissues with Endothelial Tube Networks by the Cell‐Accumulation Technique

Nishiguchi, Akihiro; Yoshida, Hiroaki; Matsusaki, Michiya; Akashi, Mitsuru

doi:10.1002/adma.201101787

Cited by 252 publications

(276 citation statements)

References 28 publications

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“…We currently developed a simple and rapid bottom-up approach, called the cell-accumulation technique, by a single cell coating using FNG nanofilms. 50 Since the FNG nanofilms prepared on individual cell surfaces provide an interactive property with the ¡ 5 ¢ 1 integrin receptor of the cell membrane, the cellcell adhesion of all seeded cells in three-dimensions can be induced at the same time (cell accumulation) (Figure 14a). …”

Section: Rapid Construction Of 3d-tissues With Endothelial Tube Networkmentioning

confidence: 99%

Development of Three-Dimensional Tissue Models Based on Hierarchical Cell Manipulation Using Nanofilms

Matsusaki

2012

Bulletin of the Chemical Society of Japan

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The creation of artificial three-dimensional (3D) tissues possessing structure and function similar to natural tissue is a key challenge for implantable tissues in tissue engineering, and for model tissues in pharmaceutical assays. This account is a summary of our current research toward this challenge. We have developed a simple and unique bottom-up approach, hierarchical cell manipulation technique, using nanometer-sized layer-by-layer films consisting of fibronectin and gelatin (FNG) as a nano-extracellular matrix (nano-ECM). The FNG nanofilms were prepared directly on the cell surface, and we discovered that at least 6 nm thick FNG films acted as a stable adhesive surface for adhesion of the second cell layer. Various 3D-layered constructs consisting of single or multiple types of cells were successfully fabricated, and the higher cellular activities induced from the 3D-structures as compared to monolayer structure were observed. Furthermore, the multilayered constructs like a blood vessel wall structure indicated almost the same drug response as in vivo natural blood vessels, suggesting the possibility to use as an in vitro blood vessel model to analyze drug response. Recently, we also developed a rapid bottom-up approach by a single cell coating using FNG nanofilms, because the fabrication of twolayers (2L) is limited through the above technique due to the time required for stable cell adhesion. This rapid approach easily provided approximately eight-layered (8L) 3D-tissues after only one day of incubation. The layer number, cell type, and location were all successfully controlled by altering the seeding cell number and order. Moreover, fully and homogeneously vascularized tissues of 1 cm width and 50¯m height were obtained by a sandwich culture of the endothelial cells. These hierarchical cell manipulations will be promising to achieve one of the dreams of biomedical field, in vitro creation of artificial 3D-tissue models.

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Section: Rapid Construction Of 3d-tissues With Endothelial Tube Networkmentioning

confidence: 99%

Development of Three-Dimensional Tissue Models Based on Hierarchical Cell Manipulation Using Nanofilms

Matsusaki

2012

Bulletin of the Chemical Society of Japan

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“…130,131 Mimicking the vascularized structures of the myocardium with various types of cell still remains one of the major challenges in TE. Some of the most commonly used methods are bottom up assembly 132 or the layer-bylayer 133,134 (LBL) approach. In a recent article by Shin et al 135 high interlayer conductivity and strong cellular adhesion was achieved in a multilayer cell construct using functional PLL-GO NPs (GONs) and the LBL approach.…”

Section: Tissue Engineeringmentioning

confidence: 99%

Current applications of graphene oxide in nanomedicine

Wu¹,

An²,

Hulme³

2015

IJN

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Graphene has attracted the attention of the entire scientific community due to its unique mechanical and electrochemical, electronic, biomaterial, and chemical properties. The water-soluble derivative of graphene, graphene oxide, is highly prized and continues to be intensely investigated by scientists around the world. This review seeks to provide an overview of the currents applications of graphene oxide in nanomedicine, focusing on delivery systems, tissue engineering, cancer therapies, imaging, and cytotoxicity, together with a short discussion on the difficulties and the trends for future research regarding this amazing material.

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“…1A). [12][13][14] The advantage of using the LbL centrifugation method is its easy handling. This technique can be easily performed by a person who is a novice in cell manipulation.…”

Section: Introductionmentioning

confidence: 99%

“…However, the centrifugation process for LbL is laborious because it requires at least seven centrifugation steps, and it potentially induces negative effects by causing some cells to grow along the hands of the centrifugal force and form cellular pellets. 14 Thus, we developed an LbL-coating process using a programmed microfluidic system. This process can reduce the volume of ECM solutions and is less laborious than the centrifugation process.…”

Section: Introductionmentioning

confidence: 99%

“…LbL ECM nanofilms were directly prepared on the cell surface, on which they acted as a stable adhesive for deposition of the second cell layer. 14 We fabricated cellular bilayers in a microfluidic channel using the proposed system. We also developed an LbL cell-coating process in a channel, and the coated cells were seeded into a culture dish.…”

Section: Introductionmentioning

confidence: 99%

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Development of Microfluidic Systems for Fabricating Cellular Multilayers

et al. 2016

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We designed a microfluidic system comprising microfluidic channels, pumps, and valves to enable the fabrication of cellular multilayers in order to reduce labor inputs for coating extracellular matrices onto adhesive cells (e.g., centrifugation). Our system was used to fabricate nanometer-sized, layer-by-layer films of the extracellular matrices on a monolayer of C2C12 myoblasts. The use of this microfluidic system allowed the fabrication of cellular multilayers in designed microfluidic channels and on commercial culture dishes. The thickness of the fabricated multilayer using this microfluidic system was higher than that of the multilayer that was formed by centrifugation. Because cellular multilayer fabrication is less laborious and the mechanical force to the cell is reduced, this novel system can be applied to tissue modeling for cell biology studies, pharmaceutical assays, and quantitative analyses of mechanical or chemical stimuli applied to multilayers.

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Rapid Construction of Three‐Dimensional Multilayered Tissues with Endothelial Tube Networks by the Cell‐Accumulation Technique

Cited by 252 publications

References 28 publications

Development of Three-Dimensional Tissue Models Based on Hierarchical Cell Manipulation Using Nanofilms

Development of Three-Dimensional Tissue Models Based on Hierarchical Cell Manipulation Using Nanofilms

Current applications of graphene oxide in nanomedicine

Development of Microfluidic Systems for Fabricating Cellular Multilayers

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