Rapid conversion of ferulic acid to 4-vinyl guaiacol and vanillin metabolites by Debaryomyces hansenii

Mathew, Sindhu; Abraham, T. Emilia; Sudheesh, Siji

doi:10.1016/j.molcatb.2006.09.001

Cited by 73 publications

(62 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Karmakar et al (2000) reported the same metabolites for FA degradation by Bacillus coagulans BK07. When FA is degraded by some microorganisms, 4-vinyl guaiacol is the major product, as observed by Mathew et al (2007) and in this study. FA degradation products at certain concentrations, including 400 μM vanillic acid, are toxic to plants (Jung et al 2004).…”

Section: Discussionsupporting

confidence: 77%

“…To investigate the physiological properties of isolates, 100 ml M-9FA liquid medium was inoculated with 1 ml bacterial culture and cultured at 37°C with shaking at 160 rpm. At regular time intervals, growth of isolates was measured in terms of the optical density of culture solutions at 600 nm (Mathew et al 2007).…”

Section: Methodsmentioning

confidence: 99%

“…M-9FA liquid medium was diluted 50-fold with distilled water and absorbance values were scanned at 200 to 400 nm (Mathew et al 2007). The maximum absorption wavelength of FA is 320 nm.…”

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Bacillus methylotrophicus isolated from the cucumber rhizosphere degrades ferulic acid in soil and affects antioxidant and rhizosphere enzyme activities

et al. 2015

View full text Add to dashboard Cite

Aims Ferulic acid (FA) accumulates in soil and inhibits plant growth. We examined whether the FA-degrading bacterium Bacillus methylotrophicus degrades FA in soil. Methods B. methylotrophicus strain CSY-F1 was isolated and applied to unplanted soil and soil planted with Cucumis sativus (cucumber). We analyzed the effects of B. methylotrophicus on rhizosphere enzyme activities and antioxidant enzyme activities in cucumber and CSY-F1 exposed to FA. Results CSY-F1 degraded FA in culture medium and in soil, giving rise to 4-vinyl guaiacol, vanillin, vanillic acid, and protocatechuic acid. When cucumber seedlings were grown in soil treated with FA, the activities of some soil enzymes decreased, and the malonaldehyde content in cucumber leaves increased. The addition of CSY-F1 to FA-treated soil increased the activities of these soil enzymes, decreased the FA concentration in the soil, and elevated the activities of some antioxidant enzymes in seedlings. Moreover, the levels of superoxide radical, hydrogen peroxide, and malonaldehyde were reduced in cucumber leaves. FA treatment increased the activities of antioxidant enzymes, including superoxide dismutase, catalase, and monodehydroascorbate, in CSY-F1. Conclusion Bacillus methylotrophicus CSY-F1 has potential applications as an FA-degrading agent in soil, as it mitigates FA stress in cucumber seedlings by activating several antioxidant and soil enzymes.

show abstract

Section: Discussionsupporting

confidence: 77%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Bacillus methylotrophicus isolated from the cucumber rhizosphere degrades ferulic acid in soil and affects antioxidant and rhizosphere enzyme activities

et al. 2015

View full text Add to dashboard Cite

show abstract

“…This mixture was further subjected to cationic polymerization at a low temperature, to yield a mixed polymer consisting of VG and 4-hydroxystyrene in 97% isolated yield. The ratio of monomer units in the polymer was determined by 31 P-NMR and was in close agreement with the ratio of the applied mixture, indicating that the copolymerization of VG and 4-hydroxystyrene proceeded smoothly. SEC confirmed the existence of a polymer with an Mn of 10,543 and a polydispersity of 2.15.…”

Section: Preparation Of Polymers From Biomasssupporting

confidence: 55%

“…Preliminary investigations of the structure of the obtained polymers was performed by 31 P-NMR after derivatization with an excess of 2-chloro-4,4,5,5-tetramethyldioxaphospholane, following a literature procedure [ 47 ]. The spectrum of PVG showed one broad signal at 139.8 ppm, indicating that polymerization of VG proceeded through the expected vinyl mechanism and no additional cross linking between the phenol units had occurred.…”

Section: Polymerization Of Vg and Characterization Of Pvgmentioning

confidence: 99%

Chemicals from agricultural biomass: chemoenzymatic approach for production of vinylphenols and polyvinylphenols from phenolic acids

Leisch

Große

Morley

et al. 2013

Green Processing and Synthesis

View full text Add to dashboard Cite

A two-step chemoenzymatic process for the preparation of polyvinylphenols from phenolic acids (PAs), being abundant aromatic constituents found in agricultural biomass, was developed. In the first step, conversion of 4-hydroxycinnamic acid derivatives to the corresponding vinylphenols, mediated by a recombinant phenolic acid decarboxylase, was evaluated using a variety of bioprocessing technologies that include biphasic whole cell and cell free extract biotransformations, a combination of biocatalyst with adsorbent resins for in situ product recovery, and fixed bed reactors using immobilized whole cells. The best yield (90%) with a high space time yield of 4.83 g/l/h was the result of a combination of crude enzyme extracts of the recombinant Escherichia coli ( E. coli ) with water immiscible organic solvents such as toluene. In the second step, cationic and radical polymerizations were tested to produce polyvinylguaiacol (PVG) from vinyl phenols. Characterization of PVG by thermogravimetric analysis (TGA), differential scanning calorimetry (DSC) and nanoindentation test are reported here for the first time. The feasibility of the chemoenzymatic process for the production of aromatic poly mers from biomass was demonstrated by the production of polymers from a mixture of ferulic acid (FA) and p -coumaric acid ( p CA), obtained from alkaline hydrolysis of corn bran. Interestingly, nanoindentation tests showed that both PVG and " mixed " PVG polymers showed significantly higher performances than a commercial polystyrene polymer.

show abstract

References

2012

Fungi and Lignocellulosic Biomass

View full text Add to dashboard Cite

Rapid conversion of ferulic acid to 4-vinyl guaiacol and vanillin metabolites by Debaryomyces hansenii

Cited by 73 publications

References 24 publications

Bacillus methylotrophicus isolated from the cucumber rhizosphere degrades ferulic acid in soil and affects antioxidant and rhizosphere enzyme activities

Bacillus methylotrophicus isolated from the cucumber rhizosphere degrades ferulic acid in soil and affects antioxidant and rhizosphere enzyme activities

Chemicals from agricultural biomass: chemoenzymatic approach for production of vinylphenols and polyvinylphenols from phenolic acids

References

Contact Info

Product

Resources

About