Rapid culture test for adenovirus isolation.

Abstract

SUMMARY A rapid culture test has been developed and evaluated for the detection of adenovirus in ocular infections. The test requires only two days' incubation of the inoculated cell monolayers on coverslips in flat bottomed tubes followed by detection of adenovirus inclusions using immunofluorescence staining method. The sensitivity of the rapid test is found to be comparable with that of the conventional tissue culture test which depends on the development of a cytopathic effect requiring 2-21 days' incubation (mean 9-4, SD 4.9).Adenovirus. ocular infections -occur sporadically throughout the year, and outbreaks are frequent both in the community and in hospitals and other institutions.

Double-label immunofluorescence method for simultaneous detection of adenovirus and herpes simplex virus from the eye

“…We have developed a similar method for the simultaneous rapid screening of multiple viral pathogens from clinical material. A single shell vial culture followed by a double-label IFA procedure allows for the possibility of detection of either or both pathogens from a single specimen within 48 h. The sensitivity of the test for the detection of ADV was equal to that of the CCT and confirms our previous findings made on the basis of use of single shell vials and a single-label IFA staining procedure (6). In this study, DLST appeared to be more sensitive than the CCT for the detection of HSV, although the difference did not reach statistical significance.…”

“…Cell culture and inoculation. Methods for cell culture and inoculation have been described previously (6,13). Briefly, HEp-2 cell monolayers were prepared by seeding at a concentration of 25,000 to 30,000 cells per ml of growth medium in cell culture test tubes and shell vials.…”

“…Shell vial systems have been described for the rapid detection of several viruses (6,11,13) and chlamydia (4) from several specimen types, but these have required separate vial cultures and separate staining procedures for the detection of each of the pathogens. We describe the development and application of a double-label shell vial test (DLST).…”

Identification and control of nosocomial adenovirus keratoconjunctivitis in an ophthalmic department

“…Adenovirus was detected by shell phial culture permitting more rapid diagnosis. In brief, Vero cells grown in shell phials were inoculated with sample, centrifuged and incubated for 48 h prior to staining with adenovirus fluorescent antibody (Imagen) and microscopic examination 6. There was no change in sample collection, transportation or the method of viral detection over the study period (1998–2005).…”

Evaluation of sampling technique and transport media for the diagnostics of adenoviral eye infections

“…More rapid assays have been developed, including enzyme immunoassay, direct immunofluorescence, DNA hybridisation and electron microscopy [4,16,17,19,22]. These techniques can be complex, laborious and often inappropriate for routine situations.…”