Rapid decontamination of gutta-percha cones with sodium hypochlorite

Cardoso, Celso Luíz; Kotaka, Cinthia Regiane; Redmerski, Roberta; Guilhermetti, Márcio; Queiroz, Alfredo Franco

doi:10.1016/s0099-2399(99)80290-8

Cited by 37 publications

(28 citation statements)

References 9 publications

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“…10,16 Nevertheless, Isci et al 9 showed that a 5.25% NaOCl solution caused physical alterations on resilon cones after 5 minutes. Moreover, Valois et al 6 observed aggressive deteriorative effects on gutta-percha cone elasticity for 5.25% NaOCl at 1 minute.…”

Section: Discussionmentioning

confidence: 99%

Effectiveness of 2% peracetic acid for the disinfection of gutta-percha cones

et al. 2011

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The aim of this study was to evaluate the effectiveness of 2% peracetic acid for the disinfection of gutta-percha cones contaminated in vitro with Escherichia coli, Staphylococcus aureus, Streptococcus mutans, Candida albicans and Bacillus subtilus (in spore form). Two hundred and twenty-five gutta-percha cones were contaminated with standardized suspensions of each microorganism and incubated at 37°C for 24 h. The cones were divided into 10 experimental groups (n = 15), according to the microorganism tested and disinfection testing times. The disinfection procedure consisted of immersing each cone in a plastic tube containing the substance. The specimens remained in contact with the substance for 1 or 2.5 minutes. Afterwards, each cone was transferred to a 10% sodium thiosulphate solution (Na 2 S 2 O 3 ) to neutralize the disinfectant. Microbial biofilms adhering to the cones were dispersed by agitation. Aliquots of 0.1 ml of the suspensions obtained were plated on Sabouraud dextrose agar, or brain and heart infusion agar, and incubated at 37°C for 24 h. The results were expressed in colony forming units (CFU/ml) and the data were submitted to the Wilcoxon Signed Rank Test (level of significance at 0.05). A significant reduction was observed, after 1 minute of exposure, in the test solution for C. albicans (p = 0.0190), S. aureus (p = 0.0001), S. mutans (p = 0.0001), B. subtilis (p = 0.0001), and E. coli (p = 0.0001). After 2.5 minutes of exposure, 100% of the microbial inocula were eliminated. It was concluded that the 2% peracetic acid solution was effective against the biofilms of the tested microorganisms on gutta-percha cones at 1 minute of exposure.

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Section: Discussionmentioning

confidence: 99%

Effectiveness of 2% peracetic acid for the disinfection of gutta-percha cones

et al. 2011

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“…It has been reported previously that 1 minute short sterilization with 5.25% NaOCl eradicated spores as well as bacteria. Because of its superior antibacterial effect, the sterilization of the GP cone with 5.25% NaOCl prior to canal filling has been recommended for a long time [4][5][6][7] . However, Short et al have shown that the cluster of cuboidal crystals on the surface of NaOClsoaked GP cone was detected at various levels and mentioned that at the time of canal filling, it might affect the apical sealing 14) .…”

Section: ⅳ Discussionmentioning

confidence: 99%

“…Recently, numerous experiments have been performed to seek chemical agents that act in a shorter time and sterilize various bacteria, and it has been reported that NaOCl, glutaraldehyde, chlorhexidine, etc. are effective [3][4][5][6][7] . However, in these experiments, GP cone was infected with artificially selective bacteria and the sterilization effect among different sterilizers was compared.…”

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confidence: 99%

Assessment of sterilization effect and the alteration of surface texture and physical properties of gutta-percha cone after short-term chemical disinfection

Pang

Jung

Yoo

et al. 2006

J Korean Acad Conserv Dent

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“…The recommended method for decontamination of GP points consists of treating the cones using 1% Sodium hypochlorite for 1 minute (Milton's solution), or 0.5% Sodium hypochlorite for 5 minutes (Dakin's solution). [3] Here, there is a risk of Sodium hypochlorite causing crystal deposition within the canals which can impede the obturation and cannot be ignored. The purpose of this study was to evaluate the effectiveness of a herbal alternative, neem, ginger, neem plus ginger and and to determine its effectiveness in decontaminating "gutta percha cones" in comparison with 5.25% sodium hypochlorite.…”

Section: Introductionmentioning

confidence: 99%

Comparative Evaluation of Antimicrobial Efficacy of Neem and Ginger, To Prove Its Effectiveness in Decontaminating “Gutta Percha Cones” - An In Vitro Study

banu.M¹,

Mathew²,

Karthick³

et al. 2018

ijcrr

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Aim:To compare and evaluate the antimicrobial efficacy of neem, ginger, neem plus ginger and 5.25% sodium hypochlorite and to determine its effectiveness in decontaminating "gutta percha cones" Materials and Methods:A concentrated extract of neem, ginger and neem plus ginger are used to check for the antimicrobial efficacy using the agar well diffusion method. Presence of zones of diffusion were identified against 3 common gutta-percha contaminants namely E.coli, E.faecalis and Staph aureus. New gutta-percha cones, freshly taken out of the packet using sterile tweezers were then decontaminated for 1minute using 6%neem, 6%ginger and 6% neem plus ginger and 5.25% sodium hypochlorite separately. Then, it was placed in a nutrient broth to check for the presence of turbidity. Results:The zones of inhibition were formed around both neem and ginger natural extract, in which neem plus ginger group showed almost equivalent antimicrobial efficacy in comparison to 5.25% Sodium hypochlorite. The broth remained clear even after 24 hours of incubation. Conclusion:We observed in this study that neem plus ginger extract is indeed effective as a GP decontaminant and it holds a promising future as a medium for storage of GP cones.

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Rapid decontamination of gutta-percha cones with sodium hypochlorite

Cited by 37 publications

References 9 publications

Effectiveness of 2% peracetic acid for the disinfection of gutta-percha cones

Effectiveness of 2% peracetic acid for the disinfection of gutta-percha cones

Assessment of sterilization effect and the alteration of surface texture and physical properties of gutta-percha cone after short-term chemical disinfection

Comparative Evaluation of Antimicrobial Efficacy of Neem and Ginger, To Prove Its Effectiveness in Decontaminating “Gutta Percha Cones” - An In Vitro Study

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