Rapid, depth-resolved light scattering measurements using Fourier domain, angle-resolved low coherence interferometry

Pyhtila, John W.; Wax, Adam

doi:10.1364/opex.12.006178

Cited by 33 publications

(18 citation statements)

References 13 publications

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“…Light scattering spectroscopy has been used in OCT to study the three-dimensional morphology of the cell nuclei [17,18]. Spectral characteristics of the objects were derived from OCT [19], and the authors of [20] studied molecules.…”

Section: Introductionmentioning

confidence: 99%

Nanosensitive optical coherence tomography for the study of changes in static and dynamic structures

Alexandrov¹,

Subhash²,

Leahy³

2014

Quantum Electron.

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Publication InformationS. Alexandrov, H. Subhash, M. Leahy (2014) 'Nanosensitive optical coherence tomography for the study of changes in static and dynamic structures'. Quantum Electronics, 44 (7):657-663. Publisher IOP ScienceLink to publisher's version http://dx.doi.org/10.1070/QE2014v044n07ABEH015487Item record http://hdl.handle.net/10379/4532Quantum Electronics 44 (7) 657 - 663 (2014) © 2014 Kvantovaya Elektronika and Turpion Ltd Abstract. We briefly discuss the principle of image formation in Fourier domain optical coherence tomography (OCT). The theory of a new approach to improve dramatically the sensitivity of conventional OCT is described. The approach is based on spectral encoding of spatial frequency. Information about the spatial structure is directly translated from the Fourier domain to the image domain as different wavelengths, without compromising the accuracy. Axial spatial period profiles of the structure are reconstructed for any volume of interest within the 3D OCT image with nanoscale sensitivity. An example of application of the nanoscale OCT to probe the internal structure of medico-biological objects, the anterior chamber of an ex vivo rat eye, is demonstrated.

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Section: Introductionmentioning

confidence: 99%

Nanosensitive optical coherence tomography for the study of changes in static and dynamic structures

Alexandrov¹,

Subhash²,

Leahy³

2014

Quantum Electron.

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“…Fourier domain a/LCI also realizes a significant increase in acquisition speed by collecting data across the entire angular range simultaneously by resolving each scattering angle using the multiple channels of an imaging spectrometer [26]. The combination of parallel collection in the depth and angular domains results in a 10 4 improvement in acquisition time, enabling interrogation of a single point in a sample in as little as 40 milliseconds.…”

Section: Fourier-domain A/lcimentioning

confidence: 99%

“…The angular distributions are compared to a theoretical basis, such as Mie theory in order to assess the most likely scattering geometry [26,29]. It has been shown that the angular distribution of the scattered intensity is related to the two-point spatial correlation function of the optical field through a Fourier transform [14] E( θ)…”

Section: Signal Analysismentioning

confidence: 99%

Nuclear Morphology Measurements with Angle-Resolved Low Coherence Interferometry For Application To Cell Biology And Early Cancer Detection

Wax

Chalut

2011

Analytical Cellular Pathology

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Abstract. The study of intact, living cells using non-invasive optical spectroscopic methods offers the opportunity to assess cellular structure and organization in a way that is not possible with commonly used cell biology imaging techniques. We have developed a novel spectroscopic technique for diagnosing disease at the cellular level based on using low-coherence interferometry (LCI) to detect the angular distribution of scattered light. Angle-resolved LCI (a/LCI) combines the ability of LCI to isolate scattering from sub-surface tissue layers with the ability of light scattering spectroscopy to obtain structural information on sub-wavelength scales. In application to examining cellular structure, a/LCI enables quantitative measurements of changes in the size and texture of cell nuclei. These quantitative measurements are characteristic of different pathological states. The capabilities of a/LCI were demonstrated using a clinical system that can be applied in endoscopic surveillance of esophageal tissue, producing high sensitivity and specificity for detecting dysplastic tissues in vivo. Experiments with in vitro cell samples also show the utility of a/LCI in observing structural changes due to environmental stimuli as well as detecting apoptosis due to chemotherapeutic agents.

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“…10 In order to enable clinical measurements, an a/LCI system based on an 18,000 element coherent (imaging) fiber bundle (Schott NA; Elmsford, NY) was developed that collected light in the spectral domain. 12 This system is able to collect and resolve light scattered from all depths in parallel in a single acquisition, decreasing the acquisition time by 3 orders of magnitude. Fourier-domain a/LCI was initially used to detect dysplasia in situ in human esophagus tissue clinically resected during esophago-gastrectomies.…”

Section: Introductionmentioning

confidence: 99%

Fourier-domain angle-resolved low coherence interferometry for clinical detection of dysplasia

2010

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Improved methods for detecting dysplasia, or pre-cancerous growth are a current clinical need, particularly in the esophagus. The currently accepted method of random biopsy and histological analysis provides only a limited examination of tissue in question while being coupled with a long time delay for diagnosis. Light scattering spectroscopy, in contrast, allows for inspection of the cellular structure and organization of tissue in vivo.Fourier-domain angle-resolved low-coherence interferometry (a/LCI) is a novel light scattering spectroscopy technique that provides quantitative depth-resolved morphological measurements of the size and optical density of the examined cell nuclei, which are characteristic biomarkers of dysplasia. Previously, clinical viability of the a/LCI system was demonstrated through analysis of ex vivo human esophageal tissue in Barrett's esophagus patients using a portable a/LCI, as was the development of a clinical a/LCI system. Data indicating the feasibility of the technique in other organ sites (colon, oral cavity) will be presented.We present an adaptation of the a/LCI system that will be used to investigate the presence of dysplasia in vivo in Barrett's esophagus patients.

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Rapid, depth-resolved light scattering measurements using Fourier domain, angle-resolved low coherence interferometry

Cited by 33 publications

References 13 publications

Nanosensitive optical coherence tomography for the study of changes in static and dynamic structures

Nanosensitive optical coherence tomography for the study of changes in static and dynamic structures

Nuclear Morphology Measurements with Angle-Resolved Low Coherence Interferometry For Application To Cell Biology And Early Cancer Detection

Fourier-domain angle-resolved low coherence interferometry for clinical detection of dysplasia

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