Rapid Detection and Identification of Bacterial Strains By Fourier Transform Near-Infrared Spectroscopy

Rodríguez-Saona, Luis E.; Khambaty, F. M.; Fry, Frederick S.; Calvey, Elizabeth M.

doi:10.1021/jf000776j

Cited by 124 publications

(112 citation statements)

References 22 publications

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“…Studies have shown that FTIR spectra can be used to identify and differentiate microorganisms down to the level of subspecies, strain, or even serotype (46)(47)(48)(49). The technique produces a complex spectral fingerprint of intact microbial cells that reflects the total biochemical composition of the microorganism.…”

Section: Identification Of Microbial Strainsmentioning

confidence: 99%

FTIR Spectroscopy for Grape and Wine Analysis

et al. 2008

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F TIR spectroscopy is a nondestructive technique that provides structural information on molecular features of a large range of compounds. Its main advantages are speed, a high degree of automation, medium resolution, and cost-effectiveness. Recent improvements in instrumentation together with advances in fiber optics and chemometrics have provided an analytical tool that is suitable for routine qualitative analysis and process control in many industries.Although FTIR spectroscopy is widely applied in the food industry, acceptance of this technology in the grape and wine industry has been relatively slow and mainly

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Section: Identification Of Microbial Strainsmentioning

confidence: 99%

FTIR Spectroscopy for Grape and Wine Analysis

et al. 2008

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“…It has already been reported that using Fourier Transform Spectroscopy identification of bacteria and other microorganisms to the level of species is possible in the near-and mid-IR [10,11]. At the same time many benefits may result fro m experimental observations of spectroscopic features in the sub-THz reg ion that was not yet widely exp lored.…”

Section: Introductionmentioning

confidence: 99%

Sub-THz Vibrational Spectroscopy of Bacterial Cells and Molecular Components

Globus¹,

Dorofeeva²,

Sizov³

et al. 2012

AJBE

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In this work, sub-terahertz (THz) spectroscopy is applied to characterize lyophilized and in vitro cultured bacterial cells of non-pathogenic species of Escherichia coli (E. coli) and Bacillus subtilis (BG), spores of BG and DNA fro m E. coli. One of the goals of this research is to demonstrate that Fourier Transform (FT) spectroscopy in the frequency region of 10-25 cm -1 is sensitive enough to reveal characteristic spectral features fro m bio-cells and spores in different environment, to verify the d ifferences between species, and to show the response of spores to vacuum and response of cultured cells to heat. The experimental technique was significantly improved for sensitivity and reliab ility. Observed spectra taken with a spectral resolution of 0.25 cm -1 using FT spectrometer with a detector operating at 1.7 K are rich in well resolved features having spectral widths of ~0.5-1 cm -1 . The reproducibility of experimental results was verified and confirmed. Measured spectra from E. coli DNA and from the entire cell have many similarit ies, thus demonstrating that the cellu lar co mponents might contribute to the v ibrational spectrum of the cell. The results of this work confirm that observed spectroscopic features are caused by fundamental physical mechanism of interaction between THz rad iation and biological macro-mo lecules. Particu larly, the analysis of results indicates that the spectroscopic signatures of microorganis ms originate fro m the co mbination of low frequency vibrational modes or group of modes at close frequencies (vibrational bands) within mo lecular co mponents of bacterial cells/spores, with the significant contribution from the DNA. The significance of this study is justified by necessity for a fast and effective, label free and reagent free optical technology to protect against environmental and other biological threats, as well as for general medical research. The obtained results show that THz v ibrational spectroscopy promises to add quantitative genetic information to the characteristic signatures of biological objects, increasing the detection accuracy and selectivity when appropriate spectral resolution is used.

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“…Sulphate and thiosulphate reducing bacteria Genus FTIRS, FTIRS-ATR HCA, PCA [16,17] Alicylobacillus Genus FTIRS PCA, SIMCA [20] Strain FTIRS-HATR LDA, PCA [75] Photorhabdus Genus FTIRS-ATR HCA, PCA [18] Xenorhabdus Genus FTIRS-ATR HCA, PCA [18] Arcanobacterium Genus FTIRS ANN [19] Actinomyces Genus FTIRS ANN [19] Trueperella Genus FTIRS ANN [19] Species FTIRS ANN [19] Bacillus Genus FTIRS PCA, SIMCA [20] Species FTIRS, FTIRS-ATR, Diffuse Reflectance-FTIRS PCA, HCA, DFA, CVA, SIMCA [26][27][28][29][30]49,50,54,55] Vegetative/Sporulated FTIRS PCA, CART [83] Escherichia Genus FTIRS-ATR HCA, PCA [18] Species FTIRS, FTIRS-ATR HCA, ANN, PCA, SIMCA, CVA [49][50][51][52][53][54]86,92] Escherichia coli Sequence type FTIRS, FTIRS-ATR PC-DFA, PLSDA, HCA, SIMCA [67,68] Strain FTIRS PCA, SIMCA, CVA [49,50,52,73] MLVA profile FTIRS HCA, CVA [72] Listeria spp. Species FTIRS, FTIRS-ATR, FTIR microspectroscopy CVA, ANN, SCDA, PLSRDA, PCA [5,[21][22][23][24][25]…”

Section: Bacteria Discrimination Level Infrared Technique Chemometricmentioning

confidence: 99%

“…Species FTIRS, FTIRS-ATR, FTIR microspectroscopy CVA, ANN, SCDA, PLSRDA, PCA [5,[21][22][23][24][25]27,49,50] Lactobacillus spp. Species FTIRS HCA, ANN [31,32,39,40,53] Enterococcus spp.…”

Section: Bacteria Discrimination Level Infrared Technique Chemometricmentioning

confidence: 99%

An Overview of the Evolution of Infrared Spectroscopy Applied to Bacterial Typing

Quintelas

Ferreira

Lopes

et al. 2017

Biotechnology Journal

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The sustained emergence of new declared bacterial species makes typing a continuous challenge for microbiologists. Molecular biology techniques have a very significant role in the context of bacterial typing, but they are often very laborious, time consuming, and eventually fail when dealing with very closely related species. Spectroscopic-based techniques appear in some situations as a viable alternative to molecular methods with advantages in terms of analysis time and cost. Infrared and mass spectrometry are among the most exploited techniques in this context: particularly, infrared spectroscopy emerged as a very promising method with multiple reported successful applications. This article presents a systematic review on infrared spectroscopy applications for bacterial typing, highlighting fundamental aspects of infrared spectroscopy, a detailed literature review (covering different taxonomic levels and bacterial species), advantages, and limitations of the technique over molecular biology methods and a comparison with other competing spectroscopic techniques such as MALDI-TOF MS, Raman, and intrinsic fluorescence. Infrared spectroscopy possesses a high potential for bacterial typing at distinct taxonomic levels and worthy of further developments and systematization. The development of databases appears fundamental toward the establishment of infrared spectroscopy as a viable method for bacterial typing.

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Rapid Detection and Identification of Bacterial Strains By Fourier Transform Near-Infrared Spectroscopy

Cited by 124 publications

References 22 publications

FTIR Spectroscopy for Grape and Wine Analysis

FTIR Spectroscopy for Grape and Wine Analysis

Sub-THz Vibrational Spectroscopy of Bacterial Cells and Molecular Components

An Overview of the Evolution of Infrared Spectroscopy Applied to Bacterial Typing

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