Rapid detection and quantitation of Bluetongue virus (BTV) using a Molecular Beacon fluorescent probe assay

Orrù, Germano; Ferrando, Maria Laura; Meloni, Mauro; Liciardi, Manuele; Savini, Giovanni; Santis, Paola De

doi:10.1016/j.jviromet.2006.05.028

Cited by 80 publications

(54 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…data) by BTV-specifi c antibody ELISAs and viral RNA detection, by using a BTV-specifi c real-time reverse transcription-PCR (rRT-PCR) (10,12). However, levels of antibody and viral RNA were unexpectedly low.…”

mentioning

confidence: 99%

Genetic Characterization of Toggenburg Orbivirus, a New Bluetongue Virus, from Goats, Switzerland

Hofmann¹,

Renzullo²,

Mader³

et al. 2008

Emerg. Infect. Dis.

274

221

View full text Add to dashboard Cite

A novel bluetongue virus (BTV) termed Toggenburg orbivirus (TOV) was detected in goats from Switzerland by using real-time reverse transcription-PCR. cDNA corresponding to the complete sequence of 7 of 10 double-stranded RNA segments of the viral genome was amplifi ed by PCR and cloned into a plasmid vector. Five clones for each genome segment were sequenced to determine a consensus sequence. BLAST analysis and dendrogram construction showed that TOV is closely related to BTV, although some genome segments are distinct from the 24 known BTV serotypes. Maximal sequence identity to any BTV ranged from 63% (segment 2) to 79% (segments 7 and 10). Because the gene encoding outer capsid protein 2 (VP2), which determines the serotype of BTV, is placed within the BTV serogroup, we propose that TOV represents an unknown 25th serotype of BTV.

show abstract

mentioning

confidence: 99%

Genetic Characterization of Toggenburg Orbivirus, a New Bluetongue Virus, from Goats, Switzerland

Hofmann¹,

Renzullo²,

Mader³

et al. 2008

Emerg. Infect. Dis.

274

221

View full text Add to dashboard Cite

show abstract

“…Quantitative PCR have also recently been developed. Different target segments are used (Orrù et al, 2006;Jimenez-Clavero et al, 2006;Toussaint et al, 2007). The sensitivity of the RT-PCR is around 1-10 copy/ml.…”

Section: Diva For Virus Identificationmentioning

confidence: 99%

Opinion of the Scientific Panel on Animal Health and Welfare (AHAW) on request from the Commission on bluetongue vectors and vaccines

2007

EFS2

View full text Add to dashboard Cite

“…rRT-PCR assays have also been reported for the detection of other viruses which cause vesicular disease of livestock including swine vesicular disease (SVD) (29), vesicular stomatitis (VS) (11,28) and vesicular exanthema of swine (VES) (31) or symptomatic look-alike diseases including bluetongue (13,27,33), bovine viral diarrhea…”

Section: Introductionmentioning

confidence: 99%

Diagnostic evaluation of a multiplexed RT-PCR microsphere array assay for the detection of foot-and-mouth and look-alike disease viruses

Hindson¹,

Baker²,

Tammero³

et al. 2007

View full text Add to dashboard Cite

A high-throughput multiplexed assay (Multiplex Version 1.0) was developed for the differential laboratory diagnosis of foot-and-mouth disease virus (FMDV) from viruses which cause clinically similar diseases of livestock. This assay simultaneously screens for five RNA and two DNA viruses using multiplexed reverse transcription PCR (mRT-PCR) amplification coupled with a microsphere hybridization array and flow-cytometric detection. Two of the seventeen primer-probe sets included in this multiplex assay were adopted from previously characterized

show abstract

Rapid detection and quantitation of Bluetongue virus (BTV) using a Molecular Beacon fluorescent probe assay

Cited by 80 publications

References 45 publications

Genetic Characterization of Toggenburg Orbivirus, a New Bluetongue Virus, from Goats, Switzerland

Genetic Characterization of Toggenburg Orbivirus, a New Bluetongue Virus, from Goats, Switzerland

Opinion of the Scientific Panel on Animal Health and Welfare (AHAW) on request from the Commission on bluetongue vectors and vaccines

Diagnostic evaluation of a multiplexed RT-PCR microsphere array assay for the detection of foot-and-mouth and look-alike disease viruses

Contact Info

Product

Resources

About