Rapid detection of anti‐Lu^b with recombinant Lu^b protein and the particle gel immunoassay

Abstract

Recombinant blood group proteins may be able to dispense with the need for RBCs for identification of certain RBC alloantibodies.

Recombinant blood group proteins facilitate the detection of alloantibodies to high‐prevalence antigens and reveal underlying antibodies: results of an international study

“…Although neither Lu8 on the Lutheran glycoprotein nor WES b on the Cr protein had been tested before, these antigens efficiently neutralized the corresponding antibodies in the inhibition assay used in this study. In general, this study and earlier studies have demonstrated that the tested rBGPs remain stable for 6 months or longer when stored at 2 to 8°C, independent of the specificity and source of the protein 3‐5,8. This also holds true when the proteins are used in highly concentrated cocktails.…”

“…§ Those antigens shown in earlier studies to be correctly expressed and capable of inhibiting antibodies with the corresponding specificities 3‐5,8,10…”

“…Ten soluble rBGPs derived from eukaryotic expression systems (Imusyn GmbH, Hannover, Germany) were used in the study (Table ). In earlier published and unpublished studies, they were designed and validated to express the most relevant polymorphic and high‐prevalence antigens of the respective blood group systems 3‐5,8,10. A cocktail of five different rBGP specificities (rPromix) containing Sc, Rg, Ch, Kn, and JMH was prepared by mixing equal volumes of the respective single‐rBGP solutions.…”

“…In particular, rBGPs may have wide applications in blood group serology as they can be used with different technical platforms, such as enzymelinked immunosorbent assay, color-coded microspheres, and protein microarrays. [2][3][4][5] Previous studies have shown that rBGPs may be of great value for identification of alloantibodies to high-prevalence blood group antigens, which is still a major challenge for immunohematology laboratories. [2][3][4][5][6][7][8][9][10][11][12] If rBGPs are used as soluble protein reagents in the hemagglutinin inhibition assay, their implementation in blood group serology is straightforward, even in routine laboratories.…”

Silencing and overexpression of human blood group antigens in transfusion: Paving the way for the next steps

“…Recombinant proteins are used for their capacity to inhibiting hemagglutination assay indicating incompatibility reaction or to trap the antibodies or ligands associated with these proteins in ELISA-type testing [20,24,[64][65][66][67].…”