Rapid detection of different DNA analytes using a single electrochemical sensor

Mills, Dawn M.; Foguel, Marcos Vinicius; Martin, Christopher P.; Trieu, Tiffany T.; Kamar, Ola; Calvo‐Marzal, Percy; Kolpashchikov, Dmitry M.; Chumbimuni‐Torres, Karin Y.

doi:10.1016/j.snb.2019.04.149

Cited by 21 publications

(19 citation statements)

References 33 publications

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“…The design of the MVF sensor was based on the 4WJ sensor design reported by us earlier . To improve the sensor's ability to interrogate highly structured RNA targets, we incorporated strand V in addition to strands M and F in the sensor design (Scheme ).…”

Section: Resultsmentioning

confidence: 99%

MVF Sensor Enables Analysis of Nucleic Acids with Stable Secondary Structures

et al. 2020

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One major challenge in nucleic acids analysis by hybridization probes is a compromise between the probe's tight binding and sequence‐selective recognition of nucleic acid targets folded into stable secondary structures. We have been developing a four‐way junction (4WJ)‐based sensor that consists of a universal stem‐loop (USL) probe immobilized on an electrode surface and two adaptor strands (M and F). The sensor was shown to be highly selective towards single base mismatches at room temperature, able to detect multiple targets using the same USL probe, and have improved ability to detect folded nucleic acids. However, some nucleic acid targets, including natural RNA, are folded into very stable secondary and tertiary structures, which may represent a challenge even for the 4WJ sensors. This work describes a new sensor, named MVF since it uses three probe stands M, V and F, which further improves the performance of 4WJ sensors with folded targets. The MVF sensor interrogating a 16S rRNA NASBA amplicon with calculated folding energy of −32.82 kcal/mol has demonstrated 2.5‐fold improvement in a signal‐to‐background ratio in comparison with a 4WJ sensor lacking strand V. The proposed design can be used as a general strategy in the analysis of folded nucleic acids including natural RNA.

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Section: Resultsmentioning

confidence: 99%

MVF Sensor Enables Analysis of Nucleic Acids with Stable Secondary Structures

et al. 2020

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“…To develop an electrochemical sensing methodology for the detection of targeted disease, many advanced technologies have been utilized, such as nanostructured immobilizing materials, transduction techniques, molecular recognition, and novel sensing arrays 446‐454 . Moreover, electrochemical sensing methods have made great advances for the detection of viruses 275,276,441,455‐458 . For example, Kaushik et al proposed that a nano‐enabled electrochemical immunosensing technology could be used for the detection of ZIKV infection in biofluid specimens collected from the infected patients, and particularly the pregnant women and newborn babies (Figure 9A).…”

Section: Zikv Detection Based On Sensing Methodsmentioning

confidence: 99%

Recent progresses and remaining challenges for the detection of Zika virus

Zhang

Chen

et al. 2021

Medicinal Research Reviews

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Zika virus (ZIKV) has emerged as a particularly notorious mosquito‐borne flavivirus, which can lead to a devastating congenital syndrome in the fetuses of pregnant mothers (e.g., microcephaly, spasticity, craniofacial disproportion, miscarriage, and ocular abnormalities) and cause the autoimmune disorder Guillain‐Barre' syndrome of adults. Due to its severity and rapid dispersal over several continents, ZIKV has been acknowledged to be a global health concern by the World Health Organization. Unfortunately, the ZIKV has recently resurged in India with the potential for devastating effects. Researchers from all around the world have worked tirelessly to develop effective detection strategies and vaccines for the prevention and control of ZIKV infection. In this review, we comprehensively summarize the most recent research into ZIKV, including the structural biology and evolution, historical overview, pathogenesis, symptoms, and transmission. We then focus on the detection strategies for ZIKV, including viral isolation, serological assays, molecular assays, sensing methods, reverse transcription loop mediated isothermal amplification, transcription‐mediated amplification technology, reverse transcription strand invasion based amplification, bioplasmonic paper‐based device, and reverse transcription isothermal recombinase polymerase amplification. To conclude, we examine the limitations of currently available strategies for the detection of ZIKV, and outline future opportunities and research challenges.

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“…Genome-based electrochemical biosensors have been among the most popular technique to detect ZIKV; this is due to their sensitivity and ability to distinguish ZIKV from dengue and other similar viruses, allowing for detection at early stages of infection [50,[118][119][120]. Genome-based electrochemical biosensors often employ nucleic acid sequence-based amplification (NASBA) before detection in order to amplify low concentrations of RNA in sample [118,119]. NASBA follows a similar process as PCR for the amplification of RNA sequences.…”

Section: Zika Virus Biosensorsmentioning

confidence: 99%

“…The authors were able to achieve a detection limit of approximately 0.3 fM and they were able to discriminate the ZIKV RNA against dengue and other similar flaviviruses (West Nile virus). Mills et al [ 119 ] used the same 4WJ method to detect for ZIKV and dengue virus separately. Using SWV, they were able to obtain an LOD of 0.98 nM of ZIKV RNA.…”

Section: Electrochemical Biosensorsmentioning

confidence: 99%

Electrochemical Biosensors for the Detection of SARS-CoV-2 and Other Viruses

2021

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The last few decades have been plagued by viral outbreaks that present some of the biggest challenges to public safety. The current coronavirus (COVID-19) disease pandemic has exponentiated these concerns. Increased research on diagnostic tools is currently being implemented in order to assist with rapid identification of the virus, as mass diagnosis and containment is the best way to prevent the outbreak of the virus. Accordingly, there is a growing urgency to establish a point-of-care device for the rapid detection of coronavirus to prevent subsequent spread. This device needs to be sensitive, selective, and exhibit rapid diagnostic capabilities. Electrochemical biosensors have demonstrated these traits and, hence, serve as promising candidates for the detection of viruses. This review summarizes the designs and features of electrochemical biosensors developed for some past and current pandemic or epidemic viruses, including influenza, HIV, Ebola, and Zika. Alongside the design, this review also discusses the detection principles, fabrication techniques, and applications of the biosensors. Finally, research and perspective of biosensors as potential detection tools for the rapid identification of SARS-CoV-2 is discussed.

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Rapid detection of different DNA analytes using a single electrochemical sensor

Cited by 21 publications

References 33 publications

MVF Sensor Enables Analysis of Nucleic Acids with Stable Secondary Structures

MVF Sensor Enables Analysis of Nucleic Acids with Stable Secondary Structures

Recent progresses and remaining challenges for the detection of Zika virus

Electrochemical Biosensors for the Detection of SARS-CoV-2 and Other Viruses

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