Rapid detection of Epstein–Barr virus DNA by loop-mediated isothermal amplification method

Iwata, Seiko; Shibata, Yukiko; Kawada, Jun; Hara, Shinya; Nishiyama, Yukihiro; Morishima, Tsuneo; Ihira, Masaru; Yoshikawa, Tetsushi; Asano, Yoshizo; Kimura, Hiroshi

doi:10.1016/j.jcv.2006.07.011

Cited by 46 publications

(39 citation statements)

References 27 publications

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“…High quality DNA is also required as a great range of contaminants inhibit the Taq DNA polymerase activity in PCR amplification. The Bst DNA polymerase is much less sensitive to components in blood products than Taq polymerases used in PCR; thus, DNA need not to be purified to the extent needed for PCR (Grab et al, 2005;Iwata et al, 2006;Ihira et al, 2007). The LAMP assay has been used to detect pathogens directly from serum samples without the DNA extraction step (Iwata et al, 2006;Ihira et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

“…The Bst DNA polymerase is much less sensitive to components in blood products than Taq polymerases used in PCR; thus, DNA need not to be purified to the extent needed for PCR (Grab et al, 2005;Iwata et al, 2006;Ihira et al, 2007). The LAMP assay has been used to detect pathogens directly from serum samples without the DNA extraction step (Iwata et al, 2006;Ihira et al, 2007). Further, DNA detection by conventional PCR requires gel electrophoresis, whereas qPCR requires excitation at a specific fluorescence wavelength range and detection at a second fluorescence wavelength range.…”

Section: Discussionmentioning

confidence: 99%

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Development of loop-mediated isothermal amplification assays for rapid and easy detection of Coxiella Burnetii

Chen

Ching

2014

Journal of Microbiological Methods

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Development of loop-mediated isothermal amplification assays for rapid and easy detection of Coxiella Burnetii

Chen

Ching

2014

Journal of Microbiological Methods

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“…LAMP method has been developed for detection of human viruses including chickenpox virus (Okamoto et al 2004 ); mumps virus (Yoshida et al 2008 ); Colorado tick fever virus Coltivirus; respiratory syncytial virus (Ushio et al 2005 ); fl avivirus causing West Nile fever ; enterovirus 71 ; human immunodefi ciency virus (HIV) causing acquired immune defi ciency syndrome (AIDS) (Hosaka et al 2009 ); pandemic (H1N1) 2009 virus (Lee et al 2010 ); cytomegalovirus causing cytomegalovirus inclusion disease (Reddy et al 2010 ); chikungunya virus (Parida et al 2007 ); Japanese encephalitis virus (Parida et al 2006 ); herpes simplex virus type 2 causing genital herpes (Enomoto et al 2005 ); adult T-cell leukemia and hairy cell leukemia viruses; human T-cell lymphotropic-1 and human T-cell lymphotropic-2 retroviruses (Komiyama et al 2009 ); foot-andmouth disease virus (Chen et al 2011 ); EpsteinBarr virus (EBV) (Iwata et al 2006 ), infl uenza A and B viruses causing infl uenza (Ito et al 2006 ); Ebola virus (Kurosaki et al 2007 ); human papilloma viruses type 6, 11, 16 and 18 (Hagiwara et al 2007 ); and HIV-1 virus (Curtis et al 2009 ). Mumps virus was circulating throughout the world, but in the United States the acceptance of MMR combined vaccine against measles, mumps, and rubella has reduced the number of patients suffering from mumps (Centers for Disease Control and Prevention 1998 ;Peltola et al 1994 ).…”

Section: Human Virusesmentioning

confidence: 99%

Rapid Detection of Viruses Using Loop-Mediated Isothermal Amplification (LAMP): A Review

Saharan

Khatri

Dingolia

et al. 2013

Biotechnology: Prospects and Applications

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Most of the diseases caused by viral infection are found to be fatal, and the diagnosis is diffi cult due to confusion with other causative agents. So, a highly effi cient molecular-based advance detection technique, i.e., loopmediated isothermal amplifi cation (LAMP) method, is developed for diagnosis of viral infections by various workers. It is based on amplifi cation of DNA at very low level under isothermal conditions, using a set of four specifi cally designed primers and a DNA polymerase with strand displacement activity. This technique is found to be superior than most of the molecular techniques like PCR, RT-PCR, and real-time PCR due to its high specifi city, sensitivity, and rapidity. Major advantage of LAMP method is its cost-effectiveness as it can be done simply by using water bath or dry bath. Here, in this review information regarding almost all the effective LAMP techniques which is developed so far for diagnosis of numerous viral pathogens is presented.

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“…The rapid detection of EBV DNA by LAMP has been developed by Iwata et al ., and Nie et al . [22,23], and RT-LAMP has been increasingly applied to rapidly diagnose infection by microbial pathogens, such as Fusarium graminearum in contaminated wheat seeds, as well as the metastasis of gastric and lung cancers [24–26]. …”

Section: Introductionmentioning

confidence: 99%

Visual Detection and Evaluation of Latent and Lytic Gene Expression during Epstein-Barr Virus Infection Using One-Step Reverse Transcription Loop-Mediated Isothermal Amplification

Tang

Wang

et al. 2013

IJMS

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Epstein-Barr virus (EBV)-associated disease exhibits distinct gene expression patterns characterized by the transcription of EBV nuclear antigen (EBNA) 1, EBNA2, latent membrane protein (LMP) 1, LMP2A, and BZLF1 (Zebra). A series of visual reverse transcript loop-mediated isothermal amplification (RT-LAMP) assays were performed to examine the expression of EBNA1, EBNA2, LMP1, LMP2A and BZLF1. The sensitivity of RT-LAMP for these transcripts was approximately equivalent to real-time RT-PCR (RT-qPCR), which was developed to quantify relative levels of EBV transcripts, and 10 to 100-fold more sensitive than conventional RT-PCR. Cross-reactions to other viruses were not observed upon examination of cell lines infected with herpes simplex viruses-1 and -2 (HSV-1 and -2), varicella zoster virus (VZV), human cytomegalovirus (HCMV) or Kaposi’s sarcoma-associated herpesvirus. When applied to 146 specimens, RT-LAMP exhibited high clinical sensitivity and specificity, with an excellent agreement (κ > 0.92) compared to RT-qPCR. These assays are convenient for rapid early diagnosis and for surveillance of EBV-infected individuals by evaluating the EBV transcriptional profile, because the results can be visualized with the naked eye. These assays may be employed in further investigations because they can aid the design of improved therapeutic regimens and can be used specifically in resource-poor settings.

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Rapid detection of Epstein–Barr virus DNA by loop-mediated isothermal amplification method

Cited by 46 publications

References 27 publications

Development of loop-mediated isothermal amplification assays for rapid and easy detection of Coxiella Burnetii

Development of loop-mediated isothermal amplification assays for rapid and easy detection of Coxiella Burnetii

Rapid Detection of Viruses Using Loop-Mediated Isothermal Amplification (LAMP): A Review

Visual Detection and Evaluation of Latent and Lytic Gene Expression during Epstein-Barr Virus Infection Using One-Step Reverse Transcription Loop-Mediated Isothermal Amplification

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