Rapid Detection of Group B Streptococci in Pregnant Women at Delivery

Bergeron, Michel G.; Ke, Danbing; nard, Christian M; Picard, François J.; Gagnon, Marie Pierre; Bernier, M.; Ouellette, Marc; Roy, Paul H.; Marcoux, Sylvie; Fraser, William D.

doi:10.1097/00006254-200101000-00009

Cited by 54 publications

(94 citation statements)

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“…Rapid polymerase chain reaction (PCR) assays, using either conventional or fluorogenic techniques, have a sensitivity of 97% and a specificity of 100% compared with traditional culture results. 24 Turnaround time for these tests ranges from 30 to 45 min for the newer fluorogenic PCR assay to 100 min for conventional PCR assays. 24 Numerous commercial PCR assays are currently available with sensitivities and specificities approaching 100%.…”

Section: Discussionmentioning

confidence: 99%

“…24 Turnaround time for these tests ranges from 30 to 45 min for the newer fluorogenic PCR assay to 100 min for conventional PCR assays. 24 Numerous commercial PCR assays are currently available with sensitivities and specificities approaching 100%. 25 The FDA has approved the use of a real-time PCR assay for detection of GBS DNA from combined vaginal and rectal swab specimens.…”

Section: Discussionmentioning

confidence: 99%

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Continued early onset group B streptococcal infections in the era of intrapartum prophylaxis

et al. 2008

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Objective: The objective of the study was to determine the rate of early onset group B streptococcus (EOGBS) infection in Utah and identify potential areas of failure in EOGBS prevention.Study Design: We queried the microbiology records of Intermountain Healthcare for infants with culture-confirmed EOGBS between 1 January 2002 and 31 May 2006 and calculated rates of EOGBS per 1000 deliveries. We reviewed the infant and maternal records of each EOGBS case to identify possible failures in EOGBS prevention.Result: There were 54 cases of EOGBS among the 127 205 births (0.42/1000 births). Of all, 12 were preterm. Of the 39 (93%) women prenatally screened for GBS, 31 (79%) had negative results and 7/8 (88%) women with positive prenatal GBS screens either did not receive intrapartum antibiotic prophylaxis (IAP) or received inadequate IAP. Of the 54 infants with EOGBS, 3 (6%) died.Conclusion: Utah's rates of EOGBS were higher than the national average. Factors associated with EOGBS include missed screening opportunities, inadequate IAP, and false-negative maternal GBS culture.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Continued early onset group B streptococcal infections in the era of intrapartum prophylaxis

et al. 2008

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“…Bergeron et al, who identified the cfb gene as a convenient target, developed a realtime PCR assay and validated its potential use in 2000. In this study, they identified GBS in vaginal-rectal swabs in less than 45 min with [95 % sensitivity and specificity when compared with culture on a selective broth medium [17]. Infectio Diagnostic (IDI, Sainte-Foy, QC, Canada) marketed the PCR developed by Bergeron et al as the IDIStrep B TM kit, running on the Cepheid Smart Cycler Ò platform (Cepheid, Sunnyvale, CA, USA).…”

Section: Polymerase Chain Reactionmentioning

confidence: 99%

“…Rapid GBS detection tests have the potential to overcome all of the above-mentioned limitations [17,18]. If performed during labor and if rapid and sensitive enough, they could obviate the need for prenatal screening and reduce the use of antibiotic prophylaxis in pregnant women who are not colonized.…”

Section: Introductionmentioning

confidence: 99%

Molecular-based Screening for Perinatal Group B Streptococcal Infection: Implications for Prevention and Therapy

2013

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Group B streptococci (GBS) are a leading cause of infectious neonatal morbidity and mortality. Timely and accurate identification of colonized pregnant women is imperative to implement intrapartum antibioprophylaxis (IAP) to reduce the risk of early neonatal sepsis. Current guidelines recommend screening for GBS carriage with vaginal-rectal cultures. However, cultures require 24-72 h, thus precluding their use for intrapartum screening and these are only performed at 35-37 weeks gestation. New rapid molecular-based tests can detect GBS within hours. They have the potential to be used intrapartum and to allow for selective IAP in women carrying GBS. An advantage is that they can sometimes be performed by non-laboratory staff in the labor suite, thus avoiding delays in sample transfers to the microbiology laboratory. Another possible use of molecular-based assays is for the diagnosis of neonatal sepsis, where tests with a short turnaround time and high sensitivity and specificity are crucial. In this situation, the detection of microorganisms once antibiotic therapy has already been started is important, as treatment is started immediately once sepsis is suspected without waiting for microbiological confirmation. In this article, we discuss the state-of-the-art molecular-based tests available for GBS screening during pregnancy, as well as their implications for IAP for the diagnosis and prevention of neonatal sepsis.

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“…Although the CDC guidelines indicate culture as the gold standard method for GBS detection, these same guidelines include expanded laboratory methods for detecting this organism. In particular, polymerase chain reaction (PCR)-based assays comprise an additional option for the rapid detection of GBS colonization 2,8 .…”

Section: Introductionmentioning

confidence: 99%

Group B Streptococcus detection in pregnant women via culture and PCR methods

Wollheim

Sperhacke

Fontana

et al. 2017

Rev. Soc. Bras. Med. Trop.

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Introduction: Group B Streptococcus (GBS), a source of neonatal infection, colonizes the gastrointestinal and genitourinary tracts of pregnant women. Routine screening for maternal GBS in late pregnancy and consequent intrapartum antibiotic prophylaxis have reduced the incidence of early-onset GBS neonatal infection. The aim of this study was to evaluate the performance of PCR, compared to culture (gold standard), in GBS colonization screening of pregnant women, and to establish the prevalence of GBS colonization among this population. Methods: Vaginal introitus and perianal samples were collected from 204 pregnant women, between the 35 th and 37 th weeks of pregnancy, at the Obstetrics and Gynecology Unit of the University of Caxias do Sul General Hospital between June 2008 and September 2009. All samples were cultured after enrichment in a selective medium and then assayed by culture and PCR methods. Results: The culture and PCR methods yielded detection rates of vaginal/perianal GBS colonization of 22.5% and 26%, respectively (sensitivity 100%; specificity 95.6%; positive and negative predictive values 86.8% and 100%, respectively). A higher prevalence of GBS colonization was detected in the combined vaginal and perianal samples by both culture and PCR assay analyses. Conclusions: PCR is a faster and more efficient method for GBS screening, allowing for optimal identification of women who should receive intrapartum antibiotic prophylaxis to prevent newborn infection.

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Rapid Detection of Group B Streptococci in Pregnant Women at Delivery

Cited by 54 publications

References 0 publications

Continued early onset group B streptococcal infections in the era of intrapartum prophylaxis

Continued early onset group B streptococcal infections in the era of intrapartum prophylaxis

Molecular-based Screening for Perinatal Group B Streptococcal Infection: Implications for Prevention and Therapy

Group B Streptococcus detection in pregnant women via culture and PCR methods

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