2005
DOI: 10.1562/2005-05-23-rc-538
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Rapid Detection of Aspergillus flavus Contamination in Peanut with Novel Delayed Luminescence Spectra

Abstract: Employing a luminescence spectrometer the usefulness of light-induced delayed luminescence (DL) for the detection of aflatoxinB1 (AfB1) contamination in whole peanut was studied. Peanut was artificially contaminated with Aspergillus flavus (7.5 3 10 5 conidia/mL) and incubated for 0-72 h. The DL and fluorescence spectra of contaminated peanut were obtained by a luminescence spectrometer. The correlation between the spectra and contamination levels was established. The DL and fluorescence intensity has a negati… Show more

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Cited by 15 publications
(10 citation statements)
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“…[20][21][22][23][24][25] On the other hand, a major experimental issue in DL spectroscopy is represented by the detection of ultra-weak light signals, specially when they come from reduced sample quantities. This can be the case for DL photo-induced on a single seed, requiring higher detection sensitivity with respect to most of present-day equipment.…”
Section: Introductionmentioning
confidence: 99%
“…[20][21][22][23][24][25] On the other hand, a major experimental issue in DL spectroscopy is represented by the detection of ultra-weak light signals, specially when they come from reduced sample quantities. This can be the case for DL photo-induced on a single seed, requiring higher detection sensitivity with respect to most of present-day equipment.…”
Section: Introductionmentioning
confidence: 99%
“…In this regard, some studies have found correlations between DL signals and color of tomato fruits at different maturity stages [ 14 ], as well as between DL intensity and fungal contamination level in peanuts artificially inoculated with A . flavus [ 44 ]. As previously reported, DL measurements were able to differentiate medicinal herbs prepared under different conditions and showing different age and therapeutic properties (these characteristics reflect the presence of different active chemical compounds) [ 21 , 22 , 40 ].…”
Section: Discussionmentioning
confidence: 99%
“…Delayed luminescence (DL) was developed as a rapid, direct, systemic tool to measure the decaying ultra-weak luminescence (up to seconds or minutes) exhibited by materials after being illuminated with light. As a sample loss-free technique, DL is a sensitive approach and widely applied in determining food quality [15], seed germination [16] and cancerous cells [17]. DL is a photo-induced ultra-weak photon emission [18], of which the properties are influenced by molecular structures and interactions [19], in particular the long chain molecules [20,21].…”
Section: Introductionmentioning
confidence: 99%