Rapid detection of <i>Listeria monocytogenes</i> using fluorescence immunochromatographic assay combined with immunomagnetic separation technique

Li, Qianru; Zhang, Sai; Cai, Yanxue; Yang, Yuexi; Hu, Fei; Liu, Xiaoyun; He, Xin

doi:10.1111/ijfs.13428

Cited by 38 publications

(17 citation statements)

References 26 publications

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“…Liu et al [17] showed that the combination of magnetic concentration and immunochromatography yields a 25-50-fold gain in the detection limit of aflatoxin M1 in milk compared to the variants in which magnetic or gold nanoparticles are used as conventional labels. A 40-fold gain in the detection limit was demonstrated by Lu et al [18] upon the detection of Listeria monocytogenes. In Petrakova et al [19], using the examples of zearalenone and T-2 toxin, the authors showed that magnetic nanoparticles can be used as directly detectable optical markers.…”

Section: Proper Sample For Lfiamentioning

confidence: 93%

Ways to Reach Lower Detection Limits of Lateral Flow Immunoassays

Zherdev¹,

Dzantiev²

2018

Rapid Test - Advances in Design, Format and Diagnostic Applications

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This chapter considers factors influencing sensitivity of lateral flow immunoassay and modern developments that are focused on reaching lower detection limits. The existing variety of proposed approaches is classified in accordance with the "big five rules" for these assays, including proper sample, receptor, interaction, response, and output. The solutions for rapid extraction of target analytes and preventing negative influence of extractants are considered. Role to antibodies affinity and specificity is characterized. Potential of alternate bioreceptor molecules is discussed. Immunoreactants' compositions, concentrations, and locations on the test strip are characterized as factors determining assay parameters. The existing variety of labels is compared in terms of their optical and alternate registration. Tools to modulate a sequence of analytical reactions and to form aggregates of the detected labels are considered. The discussed approaches are illustrated through developments of test strips for detection of mycotoxins, veterinary drugs, and other analytes.The overall design of the immunochromatographic test strip is shown in Figure 1. It is a composite of several membranes of different structures and porosities, fixed on a support. The bundling of the test strip can vary, so it makes sense to consider its design based on what analytical tasks are being performed on its different sites.A. Typically, the lower portion of the test strip contains a sample pad. It ensures the absorption of sample components, in which the presence of the target analyte is checked.B. The following is a section with immunoreagents that are washed out during the analysis and move upward along with the components of the sample. As a rule, a conjugate pad forms this zone. It contains a conjugate of antibodies against the target analyte with a nanodispersed label-particles of colored latex, colloidal gold, and so on.The next two sections are located on the main working membrane of the test strip. C.First, there is a zone along which the movement of the absorbed components of the sample and the washed immunoreagents continues. During this movement, immune reactions occur, and specific intermolecular complexes are formed. D.Next, a mixture of reacted and unreacted molecules enters the binding area with immobilized immunoreagents. Depending on whether the target analyte was present in the sample and in what amount, binding of labeled immune complexes occurs in certain areas (in the traditional case, with the formation of narrow colored lines). Usually, additional reagents are located here to control the functionality of the test system. E. The upper part of the test strip with the final pad, usually structurally similar to the sample pad, ensures the further movement of the reaction mixture under the action of capillary forces and the washing of unreacted components from the underlying areas. These processes allow the label's binding to be evaluated correctly.Membrane components of the test strip are fixed on a plastic support and partia...

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Section: Proper Sample For Lfiamentioning

confidence: 93%

Ways to Reach Lower Detection Limits of Lateral Flow Immunoassays

Zherdev¹,

Dzantiev²

2018

Rapid Test - Advances in Design, Format and Diagnostic Applications

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“…In some applications, magnetic nanoparticles have been used to perform a double function simultaneously: separation and label for optical detection [25,35,78]. In other cases, gold and fluorescent nanoparticles have been employed in combination with magnetic nanoparticles in order to use optical nanoparticles for the transduction and magnetic nanoparticles for the IMS [85,88,89]. Nanocomposites combining magnetic nanoparticles with gold or fluorescent nanomaterials have also been reported to get the same double function [84,87].…”

Section: Immunomagnetic Separation In Combination With Other Transduction Systemsmentioning

confidence: 99%

Magnetic Lateral Flow Immunoassays

et al. 2020

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A new generation of magnetic lateral flow immunoassays is emerging as powerful tool for diagnostics. They rely on the use of magnetic nanoparticles (MNP) as detecting label, replacing conventional gold or latex beads. MNPs can be sensed and quantified by means of external devices, allowing the development of immunochromatographic tests with a quantitative capability. Moreover, they have an added advantage because they can be used for immunomagnetic separation (IMS), with improvements in selectivity and sensitivity. In this paper, we have reviewed the current knowledge on magnetic-lateral flow immunoassay (LFIA), coupled with both research and commercially available instruments. The work in the literature has been classified in two categories: optical and magnetic sensing. We have analysed the type of magnetic nanoparticles used in each case, their size, coating, crystal structure and the functional groups for their conjugation with biomolecules. We have also taken into account the analytical characteristics and the type of transduction. Magnetic LFIA have been used for the determination of biomarkers, pathogens, toxins, allergens and drugs. Nanocomposites have been developed as alternative to MNP with the purpose of sensitivity enhancement. Moreover, IMS in combination with other detection principles could also improve sensitivity and limit of detection. The critical analysis in this review could have an impact for the future development of magnetic LFIA in fields requiring both rapid separation and quantification.

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“…In recent years, a growing number of studies have focused on enhancing the detection sensitivity and reducing the testing time from days to hours. The representative methods include immunology‐based methods (enzyme linked immunosorbent assay, ELISA; immunochromatographic assay, ICA), nucleic acid‐based methods (polymerase chain reaction, PCR; loop‐mediated isothermal amplification, LAMP), signal‐based methods (such as fluorochrome and quantum dots [QDs]), and so on (Bi et al., 2020; Li et al., 2017; Li et al., 2019; Park, Park, Ok, Chang, & Lim, 2020; Wei et al., 2019; Xue, Zheng, Zhang, Jin, & Lin, 2018). However, food samples are usually complex and heterogeneous systems.…”

Section: Introductionmentioning

confidence: 99%

Immunomagnetic separation: An effective pretreatment technology for isolation and enrichment in food microorganisms detection

Wang

Cai

Gao

et al. 2020

Comp Rev Food Sci Food Safe

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The high efficiency and accurate detection of foodborne pathogens and spoilage microorganisms in food are a task of great social, economic, and public health importance. However, the contamination levels of target bacteria in food samples are very low. Owing to the background interference of food ingredients and negative impact of nontarget flora, the establishment of efficient pretreatment techniques is very crucial for the detection of food microorganisms. With the sig

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Rapid detection of Listeria monocytogenes using fluorescence immunochromatographic assay combined with immunomagnetic separation technique

Cited by 38 publications

References 26 publications

Ways to Reach Lower Detection Limits of Lateral Flow Immunoassays

Ways to Reach Lower Detection Limits of Lateral Flow Immunoassays

Magnetic Lateral Flow Immunoassays

Immunomagnetic separation: An effective pretreatment technology for isolation and enrichment in food microorganisms detection

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