Rapid detection of mecA gene of methicillin-resistant Staphylococcus aureus by a novel, label-free real-time capacitive biosensor

Mahadhy, Ally; Ståhl-Wernersson, Eva; Mattìasson, Bo; Hedström, Martin

doi:10.1016/j.btre.2020.e00568

Cited by 7 publications

(6 citation statements)

References 17 publications

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“…Since our modeling is based on the experimental observation of the phenological bioelectronic signals, the abstracted fitting parameters may not hold clear physical meanings, strictly corresponding to the molecular scale entities. In other words, although our fitting results are basically in agreement with some experimental investigations using similar biointerface systems (e.g., interfacial capacitance, C EDL ), [23,26,27] the values of these parameters should not be unconditionally used as the evidence to support theoretical calculation researches, which may require more strict experimental verifications (e.g., single-molecule tests in sub-nanoscale [24b,e] ). Besides, we learned that our fitting result P P  ′ C C indicates a severe reduction of capacitance due to the complementary base pairing.…”

Section: Numerical Analysis and Quantitationsupporting

confidence: 81%

“…[6,25a,26] In comparison, previous researches using similar biomoleculeassociated interface systems have reported smaller C Helm values in the order of 10 3 nF cm -2 . [23,26,27] Therefore, the large disparity between C diff and C Helm levels results in that the EDL capacitance variables C EDL_*,* in Figure 5a can be regarded as a constant, which is approximately predominated by the Helmholtz layer C EDL ≈ C Helm (see Figure S17 in the Supporting Information for quantitative estimation details). By exploiting this important approximation, the capacitive network in Figure 5a becomes a highly periodic cascade of elementary capacitive grids including only three variables (C S , C P , and C EDL ), and supports the further simplification (see Figure S18 in the Supporting Information for equivalent circuit transformation details).…”

Section: Theoretical Elucidation and Modelingmentioning

confidence: 99%

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Observing Mesoscopic Nucleic Acid Capacitance Effect and Mismatch Impact via Graphene Transistors

Zhang

Yuan

et al. 2022

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conveyed genetic ciphers for the advanced diagnosis, treatment, and therapy of diseases. [1] Pursuing these targets, precisely and reliably locating the nucleic acids with specific sequences is a fundamental mission. [2] For example, in the recent pandemic of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), efficiently detecting target RNA fragments and base mutations is of significant importance. [3] In another area, the genomeediting technique based on the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas) systems is confronted with a critical off-target challenge. [4] To prevent nucleic-acid-editing failures and potential disasters, [5] developing a facile detection tool for the timely monitoring of baseediting outcomes, instead of the complete sequencing, would be worthwhile.Based on the Watson-Crick basepairing rules, the complementary or partial hybridization between nucleotide single strands enables the selective identification of target sequences. [6] However, the existing detection techniques are usually limited by the lack of signal generation strategies for discernible outputs. The commonly utilized nucleotide labeling typically reduces the detection instantaneity. [7] For this reason, novel label-free methods exploiting the intrinsic properties of nucleic acids, instead of extrinsic labels, are This work reports a molecular-scale capacitance effect of the double helical nucleic acid duplex structure for the first time. By quantitatively conducting large sample measurements of the electrostatic field effect using a type of high-accuracy graphene transistor biosensor, an unusual charge-transport behavior is observed in which the end-immobilized nucleic acid duplexes can store a part of ionization electrons like molecular capacitors, other than electric conductors. To elucidate this discovery, a cascaded capacitive network model is proposed as a novel equivalent circuit of nucleic acid duplexes, expanding the point-charge approximation model, by which the partial charge-transport observation is reasonably attributed to an electron-redistribution behavior within the capacitive network. Furthermore, it is experimentally confirmed that base-pair mismatches hinder the charge transport in double helical duplexes, and lead to directly identifiable alterations in electrostatic field effects. The bioelectronic principle of mismatch impact is also self-consistently explained by the newly proposed capacitive network model. The mesoscopic nucleic acid capacitance effect may enable a new kind of label-free nucleic acid analysis tool based on electronic transistor devices. The in situ and real-time nucleic acid detections for virus biomarkers, somatic mutations, and genome editing off-target may thus be predictable.The ORCID identification number(s) for the author(s) of this article can be found under https://doi.org/10.1002/smll.202105890.

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Section: Numerical Analysis and Quantitationsupporting

confidence: 81%

Section: Theoretical Elucidation and Modelingmentioning

confidence: 99%

Observing Mesoscopic Nucleic Acid Capacitance Effect and Mismatch Impact via Graphene Transistors

Zhang

Yuan

et al. 2022

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“…The low % RSD values suggest the high surface stability of the strongly electrodeposited Pty film and chemisorpted MCH-SAM on the sensor chip surfaces [32,36]. Similar results regarding reusability capacity, of about 12 to 20 times, for a Pty-ssDNA-modified sensor chip in capacitive biosensor measurements were previously recorded [6,37].…”

Section: Re-usabilitysupporting

confidence: 77%

Evaluation of Polytyramine Film and 6-Mercaptohexanol Self-Assembled Monolayers as the Immobilization Layers for a Capacitive DNA Sensor Chip: A Comparison

Mahadhy

Mattìasson

Ståhl-Wernersson

et al. 2021

Sensors

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The performance of a biosensor is associated with the properties of an immobilization layer on a sensor chip. In this study, gold sensor chips were modified with two different immobilization layers, polytyramine film and 6-mercaptohexanol self-assembled monolayer. The physical, electrochemical and analytical properties of polytyramine film and mercaptohexanol self-assembled monolayer modified gold sensor chips were studied and compared. The study was conducted using atomic force microscopy, cyclic voltammetry and a capacitive DNA-sensor system (CapSenze™ Biosystem). The results obtained by atomic force microscopy and cyclic voltammetry indicate that polytyramine film on the sensor chip surface possesses better insulating properties and provides more spaces for the immobilization of the capture probe than a mercaptohexanol self-assembled monolayer. A capacitive DNA sensor hosting a polytyramine single-stranded DNA-modified sensor chip displayed higher sensitivity and larger signal amplitude than that of a mercaptohexanol single-stranded DNA-modified sensor chip. The linearity responses for polytyramine single-stranded DNA- and mercaptohexanol single-stranded DNA-modified sensor chips were obtained at log concentration ranges, equivalent to 10−12 to 10−8 M and 10−10 to 10−8 M, with detection limits of 4.0 × 10−13 M and 7.0 × 10−11 M of target complementary single-stranded DNA, respectively. Mercaptohexanol single-stranded DNA- and polytyramine single-stranded DNA-modified sensor chips exhibited a notable selectivity at an elevated hybridization temperature of 50 °C, albeit the signal amplitudes due to the hybridization of the target complementary single-stranded DNA were reduced by almost 20% and less than 5%, respectively.

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“…The resistance genes may be transferred to other organisms, thereby strengthen the threat to the healthcare. Resistance genes can be monitored with high selectivity [ 34 ].…”

Section: Resultsmentioning

confidence: 99%

Whole Cell Recognition of Staphylococcus aureus Using Biomimetic SPR Sensors

et al. 2021

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Over the past few decades, a significant increase in multi-drug-resistant pathogenic microorganisms has been of great concern and directed the research subject to the challenges that the distribution of resistance genes represent. Globally, high levels of multi-drug resistance represent a significant health threat and there is a growing requirement of rapid, accurate, real-time detection which plays a key role in tracking of measures for the infections caused by these bacterial strains. It is also important to reduce transfer of resistance genes to new organisms. The, World Health Organization has informed that millions of deaths have been reported each year recently. To detect the resistant organisms traditional detection approaches face limitations, therefore, newly developed technologies are needed that are suitable to be used in large-scale applications. In the present study, the aim was to design a surface plasmon resonance (SPR) sensor with micro-contact imprinted sensor chips for the detection of Staphylococcus aureus. Whole cell imprinting was performed by N-methacryloyl-L-histidine methyl ester (MAH) under UV polymerization. Sensing experiments were done within a concentration range of 1.0 × 102–2.0 × 105 CFU/mL. The recognition of S. aureus was accomplished by the involvement of microcontact imprinting and optical sensor technology with a detection limit of 1.5 × 103 CFU/mL. Selectivity of the generated sensor was evaluated through injections of competing bacterial strains. The responses for the different strains were compared to that of S. aureus. Besides, real experiments were performed with milk samples spiked with S. aureus and it was demonstrated that the prepared sensor platform was applicable for real samples.

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Rapid detection of mecA gene of methicillin-resistant Staphylococcus aureus by a novel, label-free real-time capacitive biosensor

Cited by 7 publications

References 17 publications

Observing Mesoscopic Nucleic Acid Capacitance Effect and Mismatch Impact via Graphene Transistors

Observing Mesoscopic Nucleic Acid Capacitance Effect and Mismatch Impact via Graphene Transistors

Evaluation of Polytyramine Film and 6-Mercaptohexanol Self-Assembled Monolayers as the Immobilization Layers for a Capacitive DNA Sensor Chip: A Comparison

Whole Cell Recognition of Staphylococcus aureus Using Biomimetic SPR Sensors

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