2021
DOI: 10.3390/bios11050140
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Whole Cell Recognition of Staphylococcus aureus Using Biomimetic SPR Sensors

Abstract: Over the past few decades, a significant increase in multi-drug-resistant pathogenic microorganisms has been of great concern and directed the research subject to the challenges that the distribution of resistance genes represent. Globally, high levels of multi-drug resistance represent a significant health threat and there is a growing requirement of rapid, accurate, real-time detection which plays a key role in tracking of measures for the infections caused by these bacterial strains. It is also important to… Show more

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Cited by 24 publications
(11 citation statements)
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“…Indeed, the limits of detection (LoD) were 1.74, 4.19, and 0.83 CFU/mL for the three sensing units, namely, cCDOT/ZEI, cCDOT, and ZEI, respectively, with no false positives. These values are competitive with the devices in the literature [8][9][10][11][12][13][14][15][16][17]54,56 with the advantage of having sensing units that do not require specific interactions with a given analyte. This reduces fabrication costs, especially compared to biosensors.…”
Section: Discussionmentioning
confidence: 99%
“…Indeed, the limits of detection (LoD) were 1.74, 4.19, and 0.83 CFU/mL for the three sensing units, namely, cCDOT/ZEI, cCDOT, and ZEI, respectively, with no false positives. These values are competitive with the devices in the literature [8][9][10][11][12][13][14][15][16][17]54,56 with the advantage of having sensing units that do not require specific interactions with a given analyte. This reduces fabrication costs, especially compared to biosensors.…”
Section: Discussionmentioning
confidence: 99%
“…The principle is to recognize Staphylococcus aureus through micro-contact imprinting and optical sensor technology. 65…”
Section: Cosmetic Assessment (Cell Culture and In Vitro Culture)mentioning
confidence: 99%
“…Therefore, other approaches, namely, oriented surface imprinting, epitope imprinting, and dual template docking oriented molecular imprinting (DTD-OMI) have been developed. In oriented surface printing, the removal of the template will result in specific binding sites only on the polymer surface, , providing better access for the target molecule than bulk imprinting. In epitope printing, only a small portion or fragment of the target molecule (an epitope) is used as the template to create an imprint for the target, , whereby more specific and strong interactions can be obtained. This can create imprints that recognize templates, peptides, or even whole proteins .…”
Section: Introductionmentioning
confidence: 99%