Rapid Detection of Microbial Contamination in Ghanaian Herbal Medicines by PCR Analysis

Dei-Tutuwa, D; Amuna, P; Rahman, MA

doi:10.4314/gmj.v48i2.9

Cited by 11 publications

(5 citation statements)

References 13 publications

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“…Genomic DNA was extracted from E. coli isolates by the boiling method (Dei-Tutuwa and Rahman, 2014). Briefly, colonies of E. coli were suspended in 100 μl of distilled water and heated at 100°C for 10 min followed by cooling for 10 min.…”

Section: Methodsmentioning

confidence: 99%

Prevalence of Antibiotic-Resistant Escherichia coli in Drinking Water Sources in Hangzhou City

Chen

et al. 2017

Front. Microbiol.

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This study investigated the distribution of antibiotic resistant Escherichia coli (E. coli) and examined the possible relationship between water quality parameters and antibiotic resistance from two different drinking water sources (the Qiantang River and the Dongtiao Stream) in Hangzhou city of China. E. coli isolates were tested for their susceptibility to 18 antibiotics. Most of the isolates were resistant to tetracycline (TE), followed by ampicillin (AM), piperacillin (PIP), trimethoprim/sulfamethoxazole (SXT), and chloramphenicol (C). The antibiotic resistance rate of E. coli isolates from two water sources was similar; For E. coli isolates from the Qiantang River, their antibiotic resistance rates decreased from up- to downstream. Seasonally, the dry and wet season had little impact on antibiotic resistance. Spearman's rank correlation revealed significant correlation between resistance to TE and phenicols or ciprofloxacin (CIP), as well as quinolones (ciprofloxacin and levofloxacin) and cephalosporins or gentamicin (GM). Pearson's chi-square tests found certain water parameters such as nutrient concentration were strongly associated with resistance to some of the antibiotics. In addition, tet genes were detected from all 82 TE-resistant E. coli isolates, and most of the isolates (81.87%) contained multiple tet genes, which displayed 14 different combinations. Collectively, this study provided baseline data on antibiotic resistance of drinking water sources in Hangzhou city, which indicates drinking water sources could be the reservoir of antibiotic resistance, potentially presenting a public health risk.

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Section: Methodsmentioning

confidence: 99%

Prevalence of Antibiotic-Resistant Escherichia coli in Drinking Water Sources in Hangzhou City

Chen

et al. 2017

Front. Microbiol.

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“…The total bacterial DNA was extracted in a clean and sterilized environment using ZR Fungal/Bacterial MiniPrep™ Kit (Zymo Research, USA). The method of [ 21 ] with slight modification was used for the extraction. One millilitre of each sample was pipetted into sterile eppendorff tubes and centrifuged at 12,500 rpm for 10 min.…”

Section: Methodsmentioning

confidence: 99%

Identification of bacterial contaminants in polyherbal medicines used for the treatment of tuberculosis in Amatole District of the Eastern Cape Province, South Africa, using rapid 16S rRNA technique

Famewo

Clarke

Afolayan

2016

J Health Popul Nutr

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BackgroundPolyherbal medicines are used for the treatment of many diseases in many African and Asian communities. With the increasing use of these remedies, several investigations have shown that they are associated with a broad variety of residues and contaminants. This study investigates the presence of bacteria in the polyherbal medicines used for the treatment of tuberculosis (TB) in the Eastern Cape Province of South Africa.MethodsBacterial DNA was extracted from the polyherbal medicines, and a fragment of the bacterial 16S rRNA gene was amplified by PCR with universal primers 27F and 518R. The amplicons were visualised on agarose gel electrophoresis, followed by end repair and adaptor ligation. They were further purified and quantified using Library Preparation kit NEBNext® UltraT DNA Library Prep Kit for Illumina, and the amplicons were run on illumina’s MiSeq platform.ResultsDifferent bacterial species were identified in all each of the polyherbal medicines. Generally, the most prominent and common bacteria recovered from all the samples were Bacillus sp., Enterobacter sp., Klebsiella sp., Rahnella sp., Paenibacillus sp., Clostridium sp. and Pantoea sp. Others are Pseudomonas sp., Raoultella ornithinolytica, Salmonella enterica and Eubacterium moniliforme.ConclusionsThis study, thus, revealed the presence of pathogenic and non-pathogenic bacteria in the polyherbal medicines used for the treatment of tuberculosis in the study area. The implications of the findings are discussed in relation to the health care of the patients of tuberculosis in the study area, having in mind that they are immunocompromised individuals.

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“…Extraction of DNA was carried out using the boiling method according to the Dei-Tutuwa's instructions for E. coli (Dei-Tutuwa et al, 2014). Frequently detected and studied genes, the plasmid-mediated quinolone resistance (PMQR) genes [qnrA,qnrB,qnrC,qnrD,qnrS,qepA,oqxA,oqxB] and the extended-spectrum β-lactamases (ESBL; bla CTX-M , bla TEM , bla SHV , bla OXA ), were chosen to amplify.…”

Section: Resistance Dna Extraction and Analysismentioning

confidence: 99%

Baicalin inhibits Escherichia coli isolates in bovine mastitic milk and reduces antimicrobial resistance

Zhao

Yuan

Liang

et al. 2018

Journal of Dairy Science

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In this study, we aimed to evaluate the inhibitory effect of baicalin on Escherichia coli in vitro and the effects of baicalin treatment on antimicrobial resistance of the E. coli isolates. Through isolation, purification, and identification, a total of 56 E. coli strains were isolated from 341 mastitic milk samples. The study of inhibition effect of baicalin on the E. coli strains in vitro was focused on permeability and morphology of the isolates using an alkaline phosphatase kit and scanning electron microscopy. Furthermore, the resistance spectrum of the isolates to the common antimicrobial agents was tested at sub-minimum inhibitory concentrations of baicalin by the agar dilution method. Extended-spectrum β-lactamase and plasmid-mediated quinolone resistance genes were amplified by PCR before and after incubation with baicalin. The results revealed that baicalin has certain inhibitory effects on the isolates in vitro. The alkaline phosphatase enzyme activity was significantly increased from 1.246 to 2.377 U/100 mL, and the surface of E. coli was concave and shriveled. Analysis of the resistance spectrum and PCR amplification showed that, after administration with baicalin, the sensitivity of most strains to the selected antimicrobial agents was enhanced. Strikingly, the drug-resistant genes from 71.43% (40/56) of these isolates were found to have drug-resistant genes to different extents. Altogether, the current study confirmed both the inhibitory effect on Escherichia coli in vitro and the reduction of antimicrobial resistance by baicalin. This is the first comprehensive study to report on baicalin, a traditional Chinese medicine that acts on E. coli isolated from the mastitic milk samples.

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Rapid Detection of Microbial Contamination in Ghanaian Herbal Medicines by PCR Analysis

Cited by 11 publications

References 13 publications

Prevalence of Antibiotic-Resistant Escherichia coli in Drinking Water Sources in Hangzhou City

Prevalence of Antibiotic-Resistant Escherichia coli in Drinking Water Sources in Hangzhou City

Identification of bacterial contaminants in polyherbal medicines used for the treatment of tuberculosis in Amatole District of the Eastern Cape Province, South Africa, using rapid 16S rRNA technique

Baicalin inhibits Escherichia coli isolates in bovine mastitic milk and reduces antimicrobial resistance

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