Rapid detection of pesticide in milk, cereal and cereal based food and fruit juices using paper strip-based sensor

Dasriya, Vaishali; Joshi, R. Malatesha; Ranveer, Soniya; Dhundale, Vishal; Kumar, Naresh; Raghu, H. V.

doi:10.1038/s41598-021-96999-w

Cited by 20 publications

(10 citation statements)

References 24 publications

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“…Aside from the fact that they are harmful to human health. Pesticides can act as an inhibitor for enzyme activity and spore growth 24 . Cross-reactivity of pesticides such as chlorpyrifos, prefenofos, paraoxon methyl, dieldrin and endosulfan was tested at their MRL values in terms of inhibition of enzyme activity.…”

Section: Resultsmentioning

confidence: 99%

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Spore-based innovative paper-strip biosensor for the rapid detection of ß-lactam group in milk

Goel

Vishweswaraiah

Kumar

2022

Sci Rep

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The study's goal was to develop a spore-based paper strip biosensor for detecting ß-lactam antibiotics in milk using the enzyme induction principle. A new spore-based paper strip biosensor has been developed after important operating parameters such as spore volume, substrate volume, exposure time and temperature, and incubation time and temperature were optimised. The limit of detection for various ß-lactam antibiotics, including amoxicillin, penicillin, ampicillin, carbenicillin, cloxacillin, nafcillin, oxacillin, cephalothin, cefalexin, cefoxitin, cefazolin, and cefuroxime, was determined in milk with detection sensitivity of 1 ppb, 2 ppb, 2 ppb, 10 ppb, 10 ppb, 10 ppb, 20 ppb, 10 ppb 1000 ppb, 10 ppb 300 ppb and 100 ppb, respectively. It was also tested with other contaminants such non-ß-lactam antibiotics, pesticides, aflatoxin, heavy metals, and other chemical contaminants, and no interference was found, indicating that the created biosensor had a low rate of false positive and negative results. In comparison to the AOAC-approved CHARM-ROSA ß-lactam strip test, which identified 7 raw milk and zero pasteurised milk samples positive for ß-lactam antibiotics, the sensor was further analysed and verified using 200 raw milk and 105 pasteurised milk samples. This indicates a perfect match between our biosensor and the AOAC-approved CHARM-ROSA ß-lactam strip test. The developed spore-based paper strip biosensors are expected to be useful in the rapid and cost-effective detection of ß-lactam antibiotic residues in milk samples at the dairy farm, reception dock, and production units, respectively.

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Section: Resultsmentioning

confidence: 99%

“…The propagation medium was prepared by following the protocol in our recent work 24 . The stock solution of the chromogenic substrate nitrocefin was prepared in 500 µL organic solvent with the addition of 5 mg nitrocefin (Sigma Aldrich, U.S.A; HiMedia, Mumbai, India).…”

Section: Methodsmentioning

confidence: 99%

Spore-based innovative paper-strip biosensor for the rapid detection of ß-lactam group in milk

Goel

Vishweswaraiah

Kumar

2022

Sci Rep

Self Cite

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“…The studies of pesticides which include their detection, distribution in soil and water bodies, modes of action, transport mechanisms and pathways have been performed extensively [54][55][56]. Identifying the sources of pesticide residues is the key to mitigate pesticide pollution in soil and water.…”

Section: Pesticides Classification and Characteristicsmentioning

confidence: 99%

Microalgae-Enabled Wastewater Treatment: A Sustainable Strategy for Bioremediation of Pesticides

Sean

Lau

Ismail

et al. 2022

Water

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Pesticides have been identified as major contaminants of various waterways. Being classified as potential endocrine disrupting compounds, pesticides in aqueous system are highly hazardous to aquatic organisms and the ecosystem. The treatment of pesticide-containing wastewater can be performed through several means, but a wastewater treatment strategy which emphasizes both treatment efficiency and sustainability is a necessity of current time. In this context, bioremediation has been increasingly promoted as an alternative technique for the remediation of diverse pollutants. Particularly, bioremediation which involves the utilization of microalgae for the removal or conversion of pesticides to the harmless or less harmful compounds is becoming a trend. Exploiting microalgae as a tool for wastewater treatment presents multiple advantages over conventional treatment technologies, which include an opportunity to simultaneously treat pesticide-containing wastewater and nutrient recovery for microalgae cultivation as well as less formation of toxic sludge. This review discusses the roles of microalgae in mitigating pesticide pollution issue, while offering an opportunity for nutrient recovery from various wastewater sources. Based on the current laboratory studies, the use of microalgae bioremediation as a promising strategy for pesticide treatment has been rationalized. The establishment of more pilot scale studies is highly encouraged to further facilitate the implementation of this treatment approach for practical application.

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“…The adverse effects of pesticides have become increasingly prominent. Triadimefon, imidacloprid, fenitrothion, chlorpyrifos-methyl and chlorpyrifos are among the most frequently found pesticides in wheat flour according to literatures (Bordin et al, 2017;Dasriya et al, 2021;Watanabe et al, 2018).…”

Section: Open Accessmentioning

confidence: 99%

Effect of Chinese steamed bun and bread processing on pesticide residues in wheat flour

Duan

Gao³

et al. 2022

Food Prod Process and Nutr

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The changes of five pesticides including imidacloprid, triadimefon, fenitrothion, chlorpyrifos-methyl, and chlorpyrifos in wheat flour during Chinese steamed bun and bread processing were systematically investigated. The pesticide residues were determined by high performance liquid chromatography coupled with diode array detector. Dough mixing step in both Chinese steamed bun and bread processing reduced the concentration of five pesticide residues significantly by 33 to 46%. It was mainly attributed to the increase of moisture content in mixed dough during this step. The reduction of pesticides in fermenting step varied from 2 to 22% in Chinese steamed bun and bread processing. Resting step in both Chinese steamed bun and bread processing has little effect on the pesticide residues with the reduction from 2 to 8%. The five pesticides have different behaviours in steaming step of Chinese steamed bun processing and in baking step of bread processing. During the steaming step, only the concentrations of triadimefon and imidacloprid residues in crust were increased by 52 and 1%, the others in crust and in crumb of Chinese steamed bun were decreased by 4 to 38%. After the baking step, the concentrations of triadimefon and imidacloprid residues in crust, and the triadimefon residue in crumb of bread were increased by 65, 83, and 14%, respectively, the others were all reduced. The processing factors (PFs) for triadimefon and imidacloprid in crust in the steaming and baking steps, for triadimefon in crumb in the baking steps were greater than 1, and the others were all less than 1. Overall, this study provides important references for monitoring pesticide residues in the processing of wheat flour products. The PFs obtained could be helpful for the risk assessment of pesticides in wheat flour products.

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Rapid detection of pesticide in milk, cereal and cereal based food and fruit juices using paper strip-based sensor

Cited by 20 publications

References 24 publications

Spore-based innovative paper-strip biosensor for the rapid detection of ß-lactam group in milk

Spore-based innovative paper-strip biosensor for the rapid detection of ß-lactam group in milk

Microalgae-Enabled Wastewater Treatment: A Sustainable Strategy for Bioremediation of Pesticides

Effect of Chinese steamed bun and bread processing on pesticide residues in wheat flour

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