Rapid Detection of Ricin in Serum Based on Cu-Chelated Magnetic Beads Using Mass Spectrometry

Zhao, Yongqiang; Song, Jian; Wang, Hongli; Xu, Bin; Feng, Liang; He, Kun; Wang, Na

doi:10.1007/s13361-016-1340-1

Cited by 6 publications

(1 citation statement)

References 32 publications

(39 reference statements)

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“…6 Ricin is the deadliest poison with LD50 in humans of 5-10 μg/kg through inhalation or 350-700 μg for a 70 kg person. 7 This study was conducted to quantify ricin protein in Ricinus communis L. seed that is purified by CLC and FPLC using the Bradford method.…”

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confidence: 99%

Determination and Purification of Ricin Protein from Ricinus communis L. Seeds using CLC (Column Liquid Chramotagoraphy) and FPLC (Fast Protein Liquid Chramatography)

Herawati

Lesmana²,

Levita³

et al. 2021

IJPST

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Ricin is the most toxic substance isolated from Ricinus communis L. seeds. It is a heterodimeric twodomain polypeptide protein that includes chain A (30 kDa) and chain B (35 kDa) linked by a disulfide bond. Ricin binds to cells by the B chain and is then internalized. Ricin has been reported as a potential chemical for cancer treatment. The measurement of protein concentration in an aqueous sample is an important assay in biochemistry research. However, so far, the quantification of ricin protein is not much reported. In this study, the quantification of ricin protein extracted from R. communis L. from Nganjuk, East Java, Indonesia, the techniques purification was used column liquid chromatography (CLC) and fast protein liquid chromatography (FPLC), followed by quantification protein content using the Bradford method. Results showed that all purification techniques positively confirm the presence of ricin protein. Ricin protein content was 0.171±0.021 mg/mL and 0.382±0.023 mg/mL using CLC and FPLC, respectively. This study might contribute to understanding the chemical properties of the ricin protein from R. communis L. The Bradford method was used to quantify purified ricin, and the fractions obtained from FPLC were higher than those obtained from CLC.

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Determination and Purification of Ricin Protein from Ricinus communis L. Seeds using CLC (Column Liquid Chramotagoraphy) and FPLC (Fast Protein Liquid Chramatography)

Herawati

Lesmana²,

Levita³

et al. 2021

IJPST

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Ricin Analysis

Kalb¹,

Barr²

2019

Encyclopedia of Analytical Chemistry

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Ricin is a protein toxin produced by the castor bean plant, Ricinus communis . In vivo, the 64‐kDa ricin protein halts protein synthesis by depurinating 28S ribosomal RNA, rendering it unable to bind to elongation factor 2 (EF‐2). This halt in protein synthesis causes detrimental health effects and can lead to death upon exposure to ricin. Ricin is highly toxic and has the potential for high availability; therefore, it is considered a likely agent for bioterrorism. Hence, sensitive and selective analytical methods are desired to analyze for ricin in food, environmental, and clinical samples. This article reviews various published methods to detect ricin. These methods are numerous but can be loosely grouped into animal assays, detection of ricin DNA, immunoassays, mass‐spectrometry‐based amino acid composition methods, and detection of ricin enzymatic activity. These various methods have different purposes. Some methods function as rapid screens with moderate false positive and false negative rates, whereas others serve as confirmatory assays. In this article, the methods are explained and compared, with discussion on the sensitivity and selectivity of the methods.

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Ricin and RCA—The Enemies Within Castor (Ricinus communis L.): A Perspective on Their Biogenesis, Mechanism of Action, Detection Methods and Detoxification Strategies

Ashfaq

Kumar

et al. 2018

Compendium of Plant Genomes

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Rapid Detection of Ricin in Serum Based on Cu-Chelated Magnetic Beads Using Mass Spectrometry

Cited by 6 publications

References 32 publications

Determination and Purification of Ricin Protein from Ricinus communis L. Seeds using CLC (Column Liquid Chramotagoraphy) and FPLC (Fast Protein Liquid Chramatography)

Determination and Purification of Ricin Protein from Ricinus communis L. Seeds using CLC (Column Liquid Chramotagoraphy) and FPLC (Fast Protein Liquid Chramatography)

Ricin Analysis

Ricin and RCA—The Enemies Within Castor (Ricinus communis L.): A Perspective on Their Biogenesis, Mechanism of Action, Detection Methods and Detoxification Strategies

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