2017
DOI: 10.4172/2157-7579.1000415
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Rapid Detection of Type 2 Porcine Reproductive and Respiratory Syndrome Virus by a Duplex Reverse Transcription Insulated Isothermal PCR on a Field-Deployable System

Abstract: Citation: Kuo HC, Lo DY, Chen CL, Lee CH, Shen YH, et al. (2017) AbstractPorcine reproductive and respiratory syndrome virus (PRRSV) is an important porcine pathogen globally. Reverse transcription-polymerase chain reaction (RT-PCR) for PRRSV detection is an important tool for disease management and control. Clinical sensitivity of RT-PCR for PRRSV detection is compromised to a certain degree by the high genetic diversity in the PRRSV genome. A duplex RT-insulated isothermal PCR (RT-iiPCR) for the North Ame… Show more

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“…Sequence analysis was performed using BioEdit (Hall, 1999). Linkage analysis of the markers was performed using CarthaGene version 1.2 (Schiex and Gaspin, 1997). Six fulllength nucleotide sequences of DFR2 (Xu et al, 2010;Yan et al, 2014;Sundaramoorthy et al, 2016) were downloaded for comparison with our two DFR2 sequences.…”
Section: Population Structure and Relatednessmentioning
confidence: 99%
“…Sequence analysis was performed using BioEdit (Hall, 1999). Linkage analysis of the markers was performed using CarthaGene version 1.2 (Schiex and Gaspin, 1997). Six fulllength nucleotide sequences of DFR2 (Xu et al, 2010;Yan et al, 2014;Sundaramoorthy et al, 2016) were downloaded for comparison with our two DFR2 sequences.…”
Section: Population Structure and Relatednessmentioning
confidence: 99%