Rapid detection of viable Listeria monocytogenes in chilled pork by real-time reverse-transcriptase PCR

Ye, Keping; Zhang, Qiuqin; Jiang, Yun; Xu, Xinglian; Cao, Jinxuan

doi:10.1016/j.foodcont.2011.10.025

Cited by 23 publications

(4 citation statements)

References 45 publications

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“…(4) Live foodborne pathogens detection in market. To detect the existence of live Listeria monocytogenes in commercial pork, Ye et al [ 28 ] developed a real-time RT-PCR without pre-enrichment step and evaluated its reliability by comparing the detection results with those of traditional culture methods. With the advantages of fast detection speed, strong sensitivity, and wide detection range (10–10 6 CFU/mL), this method effectively detected live Listeria monocytogenes in pork.…”

Section: Whole-processes Application Of Pcr Mediated Nucleic Acid Mol...mentioning

confidence: 99%

PCR Mediated Nucleic Acid Molecular Recognition Technology for Detection of Viable and Dead Foodborne Pathogens

Chen

Lan

Zhu

et al. 2022

Foods

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Living foodborne pathogens pose a serious threat to public and population health. To ensure food safety, it is necessary to complete the detection of viable bacteria in a short time (several hours to 1 day). However, the traditional methods by bacterial culture, as the gold standard, are cumbersome and time-consuming. To break through the resultant research bottleneck, PCR mediated nucleic acid molecular recognition technologies, including RNA-based reverse transcriptase PCR (RT-PCR) and DNA-based viability PCR (vPCR) have been developed in recent years. They not only sensitively amplify detection signals and quickly report detection results, but also distinguish viable and dead bacteria. Therefore, this review introduces these PCR-mediated techniques independent of culture for viable and dead foodborne pathogen detection from the nucleic acid molecular recognition principal level and describes their whole-process applications in food quality supervision, which provides a useful reference for the development of detection of foodborne pathogens in the future.

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Section: Whole-processes Application Of Pcr Mediated Nucleic Acid Mol...mentioning

confidence: 99%

PCR Mediated Nucleic Acid Molecular Recognition Technology for Detection of Viable and Dead Foodborne Pathogens

Chen

Lan

Zhu

et al. 2022

Foods

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“…Hanning et al (2009a) detected Pseudomonas species immediately after processing, where traditional culturing methods did not yield results until day 4. However, rRNA and mRNA do have a shorter half-life compared to DNA and require more labor because they are more difficult to isolate in pure form (Scheu et al, 1998;Ye et al, 2012). However, while PCR targeting DNA can enumerate both viable and nonviable bacterial cellular DNA, the use of mRNA and rRNA as the PCR target will detect only actively growing cells (Hanning et al, 2009a;Maukonen and Saarela, 2009;Park et al, 2014;de Medici et al, 2015).…”

Section: Nucleic Acid-based Approachesmentioning

confidence: 99%

Salmonella and the Potential Role for Methods to Develop Microbial Process Indicators on Chicken Carcasses

et al. 2015

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“…Next, 5 ml of homogenate was centrifuged for 3 min at 12,000g and the upper fat in the tube carefully removed with sterile cotton sticks. The sediment was resuspended with 1 ml sterile water, and the pellet was used for bacterial DNA extraction using the TIANamp Bacteria DNA Kit (Tiangen Biotech Beijing Co., Ltd., China) as reported by Ye et al (2012). …”

Section: Extraction Of Dna For the Quantitative Real-time Polymerase mentioning

confidence: 99%

Real-Time TaqMan PCR for Rapid Detection and Quantification of Coliforms in Chilled Meat

et al. 2015

Food Anal. Methods

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Coliforms are a major group of bacteria associated with spoilage of refrigerated pork. It is necessary to develop an efficient and fast method to detect total coliforms in chilled pork because the traditional methods are laborious and timeconsuming. In this study, a quantitative real-time polymerase chain reaction (qRT-PCR) targeting the lacZ gene was developed for rapid enumeration of coliforms from chilled pork. Of the 106 strains tested, 35 coliform strains and one Shigella sonnei strain were correctly identified compared with 70 control strains which failed to amplify. Detection sensitivity was 112 CFU/g. The assay was able to detect and accurately quantify the total coliforms in chilled meat within 2 h without preenrichment. The results indicate that the real-time PCR is an efficient and time-saving method that could be adopted by the meat industry to detect coliforms to replace the traditional most probable number and the rapid coliform count plate methods.

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Rapid detection of viable Listeria monocytogenes in chilled pork by real-time reverse-transcriptase PCR

Cited by 23 publications

References 45 publications

PCR Mediated Nucleic Acid Molecular Recognition Technology for Detection of Viable and Dead Foodborne Pathogens

PCR Mediated Nucleic Acid Molecular Recognition Technology for Detection of Viable and Dead Foodborne Pathogens

Salmonella and the Potential Role for Methods to Develop Microbial Process Indicators on Chicken Carcasses

Real-Time TaqMan PCR for Rapid Detection and Quantification of Coliforms in Chilled Meat

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