Rapid detection ofCampylobacter jejuni andCampylobacter coli isolated from clinical specimens using the polymerase chain reaction

Stonnet, V.; Sicinschi, Liviu A.; Mégraud, Françis; Guesdon, Jean‐Luc

doi:10.1007/bf02116533

Cited by 19 publications

(20 citation statements)

References 13 publications

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“…In this study, the compatibility of primers and annealing temperatures were examined for multiplex PCR, which differentiated C. jejuni, C. coli, and C. lari. Various primers specific to each species of campylobacters have been developed since now [22,25,29,30,36]. We chose such primers that could amplify different targets of the gene and different sizes of amplicon to avoid non-specific amplification and competition.…”

Section: Discussionmentioning

confidence: 99%

“…are now one of the most common causes of human bacterial gastroenteritis in the whole world [30,31]. Campylobacteriosis is mainly a food-borne infection, and poultry products may play an important role in the transmission to humans [28].…”

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confidence: 99%

“…Polymerase chain reaction (PCR) can idenfity the bacteria on a gene level. Some primers specific to each of C. jejuni, C. coli, and C. lari have already been developed [22,25,29,30,36] and their functions verified. Harmon et al [14] have established a PCR method that can differentiate C. coli from C. jejuni and Wesley et al [35] have succeeded in identifying C. jejuni and C. coli in pigs by the method, but detection of thermophilic campylobacters from birds has not been achieved until now.…”

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confidence: 99%

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Detection of Thermophilic Campylobacter from Sparrows by Multiplex PCR: The Role of Sparrows as a Source of Contamination of Broilers with Campylobacter.

2000

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ABSTRACT. The best combination of primers and the annealing temperature of multiplex PCR for Campylobacter jejuni, Campylobacter coli, and Campylobacter lari were examined. The multiplex PCR was able to detect type strains of the three species. All results of identification of wild strains (30 strains of C. jejuni, 20 strains of C. coli, and 4 strains of C. lari) by the multiplex PCR coincided with those of the conventional biochemical identification tests, suggesting that the multiplex PCR can simultaneously differentiate C. jejuni, C. coli, and C. lari from wild strains of campylobacters easily and rapidly. Campylobacters were detected from sparrow feces by the multiplex PCR and antimicrobial sensitivities of the strains were determined to discuss the role of sparrows in contamination of broilers with C. jejuni. Three out of 13 strains of C. jejuni isolated from sparrow feces showed quinolone resistance. From the frequent use of quinolones for treatment of industrial animals like chickens, pigs, and cows, the three strains of quinolone-resistant C. jejuni in sparrows must have been originated from those industrial animals. Sparrows that have quinolone-resistant C. jejuni were considered to have contacted with industrial animals or thier feed. It may be presumed, on the contrary, that C. jejuni in sparrows could be a potential source of contamination of broilers.

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Section: Discussionmentioning

confidence: 99%

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Detection of Thermophilic Campylobacter from Sparrows by Multiplex PCR: The Role of Sparrows as a Source of Contamination of Broilers with Campylobacter.

2000

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“…Six PCR tests for C. jejuni (4,8,9,13,32,34), four for C. coli (8,9,13,33), and a multiplex assay designed for concurrent identification and differentiation of both species (38) Tables 2 and 3). Strains giving inappropriate results were reexamined by use of the original heated lysate and also purified DNA.…”

Section: Methodsmentioning

confidence: 99%

“…A wide range of PCR assays for C. jejuni and C. coli have been described, several of which are based on a variety of genes (e.g., 23S rRNA, ceuE, and mapA) (8,9,34) and others of which are derived from different randomly generated fragments (4,(32)(33)37) (see Table 1). The sensitivity and specificity of each PCR test have been examined, but the bases of the evaluations differed significantly, particularly with respect to the selection of strains of C. jejuni, C. coli, and Campylobacter lari, a group of species that are closely related by phylogenetic and genetic criteria (21).…”

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Evaluation of 11 PCR Assays for Species-Level Identification of Campylobacter jejuni and Campylobacter coli

On¹,

Jordan²

2003

J Clin Microbiol

106

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We examined the sensitivity and specificity of 11 PCR assays described for the species identification of Campylobacter jejuni and Campylobacter coli by using 111 type, reference, and field strains of C. jejuni, C. coli, and Campylobacter lari. For six assays, an additional 21 type strains representing related Campylobacter, Arcobacter, and Helicobacter species were also included. PCR tests were initially established in the laboratory by optimizing conditions with respect to five type and reference strains of C. jejuni, C. coli, and C. lari. One PCR test for C. coli failed to give appropriate results during this initial setup phase and was not evaluated further. The remaining 10 assays were used to examine heated lysate and purified DNA templates as appropriate of well-characterized type, reference, and field strains of C. jejuni (n ‫؍‬ 62), C. coli (n ‫؍‬ 34), and C. lari (n ‫؍‬ 15). The tests varied considerably in their sensitivity and specificity for their respective target species. No assay was found to be 100% sensitive and/or specific for all C. jejuni strains tested, but four assays for C. coli gave appropriate responses for all strains examined. Between one and six strains of C. jejuni gave amplicons in four of seven C. jejuni PCR tests only where purified DNA was used as the template; corresponding results were seen with one strain of C. coli in each of three assays for the latter species. Our findings indicate that a polyphasic strategy for PCR-based identification should be used to identify C. jejuni and C. coli strains. The data may assist laboratories in selecting assays suited for their needs and in designing evaluations of future PCR tests aimed to identify these species.

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Identification of Strains of Campylobacter jejuni and Campylobacter coli by PCR and Correlation with Phenotypic Characteristics

Occhialini

Stonnet²,

Hua

et al. 1996

Campylobacters, Helicobacters, and Related Organisms

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Rapid detection ofCampylobacter jejuni andCampylobacter coli isolated from clinical specimens using the polymerase chain reaction

Cited by 19 publications

References 13 publications

Detection of Thermophilic Campylobacter from Sparrows by Multiplex PCR: The Role of Sparrows as a Source of Contamination of Broilers with Campylobacter.

Detection of Thermophilic Campylobacter from Sparrows by Multiplex PCR: The Role of Sparrows as a Source of Contamination of Broilers with Campylobacter.

Evaluation of 11 PCR Assays for Species-Level Identification of Campylobacter jejuni and Campylobacter coli

Identification of Strains of Campylobacter jejuni and Campylobacter coli by PCR and Correlation with Phenotypic Characteristics

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