Rapid determination of abamectin in lettuce and cucumber by high-performance liquid chromatography

Vuik, Johan

doi:10.1016/s0021-9673(01)88500-9

Cited by 14 publications

(9 citation statements)

References 8 publications

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“…The maximum residue limit (MRL) for emamectin benzoate in crops was officially established by the Ministry of Health and Welfare in November 1999 (The MRLs are 0.1 ppm for radish, cabbage, broccoli, tomato, eggplant and cucumber, and 0.5 ppm for tea.). Abamectin, which is applied to crops in some countries, 3,6,7) has not been allowed as pesticide in Japan, but both abamectin and ivermectin are currently used for parasite of animals. 8,9) Several methods have been reported for determining the residual macrocyclic lactones in crops.…”

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confidence: 99%

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Simultaneous Determination of Emamectin, its Metabolites, Milbemectin, Ivermectin and Abamectin in Tomato, Japanese Radish and Tea by LC/MS

Yoshii

Ishimitsu

Tonogai

et al. 2004

JOURNAL OF HEALTH SCIENCE

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We developed a simple and rapid analytical method for determining the residues of emamectin benzoate, milbemectin, abamectin, ivermectin and emamectin metabolites in tomato, Japanese Radish and Japanese tea by liquid chromatography/mass spectrometer (LC/MS) with electrospray ionization (ESI). A sample extracted with acetone was simply cleaned up using only a Sep-Pak C18, and then directly measured by LC/MS (ESI). Several LC/ MS measurement conditions were studied that included the mobile phase, solvent for sample solution, range of calibration and standard deviation of the measurement. Detecting target macrocyclic lactone chemicals with methanol as the mobile phase was more sensitive than acetonitrile, especially, milbemectin. The detection limits of these chemicals were 0.1 to 0.5 ng/ml (10 µl injection), and they were similar or more sensitive to the previous fluorescence detection method. For the measurement of these chemicals in tomato, Japanese radish and tea by LC/MS (ESI), ion suppression was always observed. To compensate for such an effect, we used a matrix-matched calibration. This compensation method is effective for obtaining accurate values. The recoveries by the developed method were in an acceptable range for the screening method and 90.1-120.9% except for milbemectin and abamectin from tea leaves that had interfering peaks.

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confidence: 99%

“…8,9) Several methods have been reported for determining the residual macrocyclic lactones in crops. 6,7,10,11) Liquid chromatographic methods using a UV detector was reported for determining abamectin residues in vegetables. 6) This method…”

mentioning

confidence: 99%

Simultaneous Determination of Emamectin, its Metabolites, Milbemectin, Ivermectin and Abamectin in Tomato, Japanese Radish and Tea by LC/MS

Yoshii

Ishimitsu

Tonogai

et al. 2004

JOURNAL OF HEALTH SCIENCE

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“…All the drugs belonging to the class are used for controlling helminthes and ectoparasities in animals [2][3] .…”

Section: Ivermectinmentioning

confidence: 99%

Quantitative Determination of Ivermectin in Raw Milk Using Positive ESI LC‐MS/MS

Dahiya

Sen

Lamba

et al. 2010

Journal of Chemistry

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Ivermectin, a veterinary drug, is commonly used endectocide for animal husbandry. The drug is available in the form of subcutaneous or topical formulations. Its application may cause accumulation of its residues into the animal tissues, which ultimately find their way into the food products, such as milk and meat products. In order to determine the residues of ivermectin in milk, a comparatively simple, sensitive and rapid method was developed and validated using LC-MS/MS. The MRM transitions corresponding to m/z 892.71>569.6, 892.71>551.5 and 892.71>307.3 were used for the purpose of quantification and evaluation of other parameters of the method. The limit of detection of the method was found to be 0.1 µg/kg and the limit of quantitation was calculated as 0.2 µg/kg. The method was found to be linear in the range of 1.0 ng/mL to 100.0 ng/mL with correlation coefficient of 0.9992 for pure calibration curve and 0.9990 for the matrix-matched calibration curve. The recoveries of ivermectin from the spiked samples of raw milk were found between 85 to 105%.

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“…Avermectins, a family of pesticide agents, are a product of the fermentation process with the microorganism Streptomyces avermetilis, and caused significant changes in the control of helminths and ectoparasitis in animals. 2,3 The compounds abamectin and ivermectin, shown in Figure 1, are macrocyclic lactones. Abamectin, a precursor to ivermectin, differs from ivermectin in that it has a double-bond at the C [22][23] position.…”

Section: Introductionmentioning

confidence: 99%

“…4 Abamectin is constituted of a mixture of two homologues, having 80% of avermectin B 1a and not more than 20% of avermectin B 1b . 3 Abamectin was developed as an insecticide and acaricide for contact and stomach action and is highly effective against gastrointestinal nematodes of cattle, 4,5 but is not approved for use in dairy animals. 6 Ivermectin is derived from abamectin and consists of a mixture of two homologues: 5-O-dimethyl-22,23-dihydroavermectin B 1a and B 1b in a ratio of 80:20.…”

Section: Introductionmentioning

confidence: 99%

Rapid and accurate simultaneous determination of abamectin and ivermectin in bovine milk by high performance liquid chromatography with fluorescence detection

Kolberg¹,

Presta²,

Wickert³

et al. 2009

J. Braz. Chem. Soc.

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Um método analítico empregando cromatografia líquida de alta eficiência com detecção por fluorescência para a determinação simultânea de abamectin e ivermectin em leite bovino foi desenvolvido e validado. Os melhores resultados de recuperação foram obtidos empregando acetonitrila na extração dos compostos, com purificação do extrato por extração em fase sólida em cartuchos contendo C 18 . A derivação pré-coluna foi realizada com N-metilimidazol e anidrido trifluoracético. Os limites de detecção (LOD) do método para abamectin e ivermectin foram 0,10 e 0,14 µg L -1 e os limites de quantificação (LOQ) foram 0,18 e 0,36 µg L -1, respectivamente. As recuperações foram de 75 a 101%, com valores de RSD abaixo de 10%. Os valores de LOD e LOQ obtidos são inferiores aos limites máximos de resíduos em leite estabelecidos pelo Codex Alimentarius, União Européia e legislação brasileira.An analytical method using high performance liquid chromatography with fluorescence detection for the simultaneous determination of abamectin and ivermectin in bovine milk was developed and validated. The best recovery results were achieved by using acetonitrile for extraction of the compounds followed by solid phase extraction in cartridges containing C 18 for the purification of the extract. Pre-column derivatization was accomplished with N-methylimidazole and trifluoroacetic anhydride. The method limit of detection (LOD) values for abamectin and ivermectin were 0.10 and 0.14 µg L -1 and the limit of quantification (LOQ) values were 0.18 and 0.36 µg L -1 , respectively. The recoveries were from 75 to 101%, with RSD values lower than 10%. The LOD and LOQ values are lower than the maximum residue limits (MRLs) in milk established by Codex Alimentarius, European Union and the Brazilian legislation.

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Rapid determination of abamectin in lettuce and cucumber by high-performance liquid chromatography

Cited by 14 publications

References 8 publications

Simultaneous Determination of Emamectin, its Metabolites, Milbemectin, Ivermectin and Abamectin in Tomato, Japanese Radish and Tea by LC/MS

Simultaneous Determination of Emamectin, its Metabolites, Milbemectin, Ivermectin and Abamectin in Tomato, Japanese Radish and Tea by LC/MS

Quantitative Determination of Ivermectin in Raw Milk Using Positive ESI LC‐MS/MS

Rapid and accurate simultaneous determination of abamectin and ivermectin in bovine milk by high performance liquid chromatography with fluorescence detection

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