Rapid determination of aflatoxin B1 by an automated immunomagnetic bead purification sample pretreatment method combined with high‐performance liquid chromatography

Abstract

We aimed to establish an automated versatile sample preconcentration method based on the modified immunomagnetic beads, which was utilized to enrich for aflatoxin B1 from the matrices. The critical main parameters affecting the extraction efficiency, such as usage amount of immunomagnetic beads, reaction time, elution time, and blending way were investigated. Under the optimized conditions, the content of aflatoxin B1 was analyzed by high‐performance liquid chromatography, the mobile phase consists of water–acetonitrile–methanol (42:18:10, v/v/v), and fluorescence detection was performed with excitation and emission wavelengths at 360 and 440 nm, respectively. Moreover, the performance of preconcentration method was compared with the conventional method based on the immunoaffinity column. The accuracy of two clean‐up methods was within the error range. In addition, the stability and recyclability of the immunomagnetic beads was studied by recycling them five times. The results for the respective analysis in various samples demonstrated that the developed extraction platform provides a promising approach that is simple, rapid, sensitive, and easy to use.

Characterization of a Trametes versicolor aflatoxin B1-degrading enzyme (TV-AFB1D) and its application in the AFB1 degradation of contaminated rice in situ

“…The supernatant was collected by centrifugation at 6,000 rpm for 10 min. Fat, protein, pigment, and carbohydrate in the extraction solution were removed by a solid-phase column for AF purification ( Zhang et al, 2020 ). AFB 1 detection was performed by the HPLC method using the following parameters: a mobile phase of 4:6 methanol–water, C18 reversed-phase column with a UV detector, a detection wavelength of 365 nm, and a flow rate of 0.6 ml/min.…”

Detection of AFB1 in corn by MXene paper‐based unlabeled aptasensor

“…The Chinese national standards specifics a maximum limit of less than 20 μg/kg in food in corn, corn flakes, and corn products. The common laboratory methods for detecting AFB1 are immunochromatography (Liu et al, 2020), chemiluminescence (Shim et al, 2014), high‐performance liquid chromatography (Zhang et al, 2020), chromatography–mass spectrometry (Dong et al, 2018), and so on (Qu et al, 2021). However, these approaches are time‐consuming and expensive and require sample preparation and trained personnel to operate the instruments.…”