Rapid determination of arsenic species in freshwater organisms from the arsenic-rich Hayakawa River in Japan using HPLC-ICP-MS

Miyashita, Shin-ichi; Shimoya, Masahito; Kamidate, Yoshiaki; Kuroiwa, Takayoshi; Shikino, Osamu; Fujiwara, Shoko; Francesconi, Kevin A.; Kaise, Toshikazu

doi:10.1016/j.chemosphere.2009.01.029

Cited by 80 publications

(38 citation statements)

References 30 publications

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“…The most abundant and common arsenic compounds are arsenobetaine, the major arsenical in marine animals, and arsenosugars, which are dominant in marine algae. [1] Arsenobetaine and arsenosugars have also been identified in freshwater [2][3][4] and terrestrial organisms, [5] but generally only at low concentrations.…”

Section: Introductionmentioning

confidence: 99%

Biosynthesis of arsenolipids by the cyanobacterium Synechocystis sp. PCC 6803

et al. 2014

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Environmental context. Arsenic biotransformation processes play a key role in the cycling of arsenic in aquatic systems. We show that a freshwater cyanobacterium can convert inorganic arsenic into arsenolipids, and the conversion efficiency depends on the arsenic concentration. The role of these novel arsenic compounds remains to be elucidated.Abstract. Although methylated arsenic and arsenosugars have been verified in various freshwater organisms, lipidsoluble arsenic compounds have not been identified. Here, we report investigations with the model organism cyanobacterium Synechocystis sp. PCC 6803 wild type and DarsM (arsenic(III) S-adenosylmethionine methyltransferase) mutant strain, which lacks the enzymes for arsenic methylation cultured in various concentrations of arsenate (As V ). Although Synechocystis accumulated higher arsenic concentrations at the higher exposure levels, the bioaccumulation factor decreased with increasing As V . The accumulated arsenic in the cells was partitioned into water-soluble and lipidsoluble fractions; lipid-soluble arsenic was found in Synechocystis wild type cells (3-35 % of the total depending on the level of arsenic exposure), but was not detected in Synechocystis DarsM mutant strain showing that ArsM was required for arsenolipid biosynthesis. The arsenolipids present in Synechocystis sp. PCC 6803 were analysed by high performance liquid chromatography-inductively coupled plasma-mass spectrometry, high performance liquid chromatographyelectrospray mass spectrometry, and high resolution tandem mass spectrometry. The two major arsenolipids were characterised as arsenosugar phospholipids based on their assigned molecular formulas C 47 H 88 O 14 AsP and C 47 H 90 O 14 AsP, and tandem mass spectrometric data demonstrated the presence of the phosphate arsenosugar and acylated glycerol groups.Additional keyword: arsenic.

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Section: Introductionmentioning

confidence: 99%

Biosynthesis of arsenolipids by the cyanobacterium Synechocystis sp. PCC 6803

et al. 2014

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“…As primary producers, phytoplankton and marine macroalgae accumulate inorganic arsenic and biotransform them into complex organic molecules that are either water-or lipid-soluble arsenic compounds. When fed upon by the higher trophic level, the water-and lipid-soluble organoarsenicals are further metabolized into other distinct arsenicals and/or they are likely accumulated unchanged [8][9][10]. In the arsenic biogeochemical cycle, the formation of organoarsenicals via biomethylation of arsenic is considered to be a detoxification process in the marine and aquatic environment [2,10].…”

Section: Introductionmentioning

confidence: 99%

Closed Anaerobic Biotransformation Products of Organoarsenic Compounds inFucus distichus

Ojo

Onasanya

2013

ISRN Environmental Chemistry

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The closed anaerobic decomposition extracts of Fucus distichus incubated with seawater and sediment, and without sediment as control, were subjected to extractions and isolation on Sephadex LH 20 and Cellulose Thin Layer Chromatography. The decomposition extracts and isolates were analyzed by using both the Hydride Generation Gas Chromatography Atomic Absorption Spectrometry (HG-GC-AAS) and High Performance Liquid Chromatography Inductively Coupled Plasma Mass Spectrometry (HPLC-ICPMS) to identify the arsenic species in the equilibrium mixtures of the seaweed and filtrates separately. In the methanol seaweed extract, equilibrium mixture of arsenosugars (AS) AS1 and AS2 and their biotransformation products of dimethylarsinoylethanol (DMAE) and dimethylarsinic acid (DMAA) were identified. In the methanol filtrate extract of the mixture, only DMAE and DMAA were identified. However, in the control methanol filtrate extract five organoarsenic species, AS1 and AS2, one unidentified hidden organoarsenic species, DMAE and DMAA were identified in the equilibrium mixture. This result confirmed that the hidden organoarsenic species in Fucus distichus, AS1 and AS2, and an unidentified organoarsenic compounds are biotransformed to only DMAE and DMAA under an anaerobic condition. This also suggests that DMAE and DMAA are strong intermediate candidates for the generation of arsenobetaine, from arsenoribosides in the marine food webs.

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“…The assessment of arsenic species in river water has been a topical research theme. Miyashita and co-workers studied an arsenic rich river in Japan [38] addressing both water samples (river water, hot spring water) and biologi- cal samples (algae, water bug, fish). For HPLC separation of arsenic species (As(III), As(V), MMA, DMA, AB, AC, arsenosugars), a reversed phase separation was implemented using a mobile phase containing sodium 1-butansulfonate (10 mM), tetramethylammonium hydroxide (4 mM), malonic acid (4 mM) and methanol (0.5%) at a pH 3.…”

Section: Speciesmentioning

confidence: 99%