Rapid Determination of Leaf Chlorophyll Concentration, Photosynthetic Activity and NK Concentration of Elaies guineensis Via Correlated SPAD-502 Chlorophyll Index

Abstract

Determination of chlorophyll contents often requires destructives analysis and involves lengthy procedures while measurement on photosynthetic rate requires specialized equipment that often limits extensive in-field application. SPAD chlorophyll meter gives indirect measurement of leaf chlorophyll contents in a rapid, non-destructive and convenient manner. The use of SPAD meter for each individual plant species require calibration curve for each individual plant species, as the accuracy of the estimation are highly affected by the leaf anatomical characteristics. This study reports the result of correlating SPAD-502 chlorophyll index readings to the actual extracted chlorophyll content and photosynthetic rate of oil palm leaf as well as the leaf nitrogen and potassium concentration. Concentration of oil palm leaf nitrogen and chlorophyll pigment as well as photosynthetic rate were highly correlated to SPAD-502 chlorophyll index with R 2 of above 0.80. With good calibration equation, the SPAD-502 chlorophyll meter are extremely versatile for rapid determination of oil palm leaf nitrogen and chlorophyll concentration as well as photosynthetic rate.

Variation in Macronutrient Content, Phytochemical Constitution and In Vitro Antioxidant Capacity of Green and Red Butterhead Lettuce Dictated by Different Developmental Stages of Harvest Maturity

“…The non-destructive measurements of chlorophyll content (SPAD Index) [30] measured by El-nakhel et al [31], reflected the changes obtained regarding chlorophyll pigment measurements in our work. Indeed, chlorophyll increased significantly with lettuce maturation, and in both harvest stages was significantly higher in red Salanova, which was the exact same trend observed with chlorophyll measurements of lettuce at 4 and 19 DAT [31].…”

“…Indeed, chlorophyll increased significantly with lettuce maturation, and in both harvest stages was significantly higher in red Salanova, which was the exact same trend observed with chlorophyll measurements of lettuce at 4 and 19 DAT [31]. In photosynthetic tissues, carotenoids, and chlorophylls a and b operate in light harvesting and perform tasks in photo-protection by quenching free radicals, singlet oxygen, and other reactive species [30]. In our case, this is expected with lettuce growth, accompanied by an increase in the plants leaf area, implicating a higher light interception by the plants and consequently a higher need of light harvesting and photo-protection.…”

The Effectiveness of a Dark Septate Endophytic Fungus, Cladophialophora chaetospira SK51, to Mitigate Strawberry Fusarium Wilt Disease and With Growth Promotion Activities

“…The increase in chlorophyll content indicated that the photosynthetic rate was increased and led to plant health, growth promotion, and fruit production, as shown by the growth parameters measured in this study ( Table 3). Chlorophyll content (SPAD-502) was highly correlated with photosynthetic rate as well as the leaf nitrogen and potassium concentrations (Sim et al, 2015). Nitrogen plays a pivotal role in the inorganic nutrition of plants, determining growth (Mengel, 1994;Marschner, 1997) and as a major component of chlorophyll (Wang et al, 2004).…”

Development of Aerial Online Intelligent Plant Monitoring System for Oil Palm (Elaeis guineensis Jacq.) Performance to External Stimuli

“…The plants total chlorophyll content influences its photosynthesis rate [46], which correlated to the productivity [47]. The chlorophyll content results from the measurements then compared with the leaf greenness as observed by the drone camera.…”