Rapid Determination of Macrolide and Lincosamide Resistance in Group B Streptococcus Isolated from Vaginal-Rectal Swabs

Cruz, Wilfred P. dela; Richardson, Joann Y.; Broestler, Judith M.; Thornton, Jennifer A.; Danaher, Patrick

doi:10.1155/2007/46581

Cited by 10 publications

(4 citation statements)

References 23 publications

(19 reference statements)

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“…In addition, in current study, both erm and mef (A/E) genes were identified in erythromycin-resistant isolates, with erm (B) dominating. We observed a higher level of discordance between genotype and phenotype in S. pyogenes , and there were erm (B) genes in 9 erythromycin-susceptible isolates, as consistent with previous report in S. agalactiae (Dela Cruz et al, 2007 ) and SDSE isolates (Lu et al, 2016 ), hinting an alternate mechanism of resistance or a potential mutation in the 23S Rrna (Dela Cruz et al, 2007 ). Furthermore, 90.3% (421/466) harbored the erm (B) gene in S. pyogenes strains isolated from Beijing children (Liang et al, 2012 ).…”

Section: Discussionsupporting

confidence: 91%

High Prevalence of Macrolide-resistance and Molecular Characterization of Streptococcus pyogenes Isolates Circulating in China from 2009 to 2016

Fang

Fan

et al. 2017

Front. Microbiol.

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Streptococcus pyogenes, or group A Streptococcus, is a pathogen responsible for a wide range of clinical manifestations, from mild skin and soft tissue infections and pharyngitis to severe diseases. Its epidemiological characteristics should be comprehensively under surveillance for regulating the national prevention and treatment practice. Herein, a total of 140 S. pyogenes, including 38 invasive and 102 noninvasive isolates, were collected from infected patients in 10 tertiary general hospitals from 7 cities/provinces in China during the years 2009–2016. All strains were characterized by classical and molecular techniques for its emm types/subtypes, virulent factors and antibiotic resistance profiling. Of 140 isolates, 15 distinct emm types and 31 subtypes were detected, dominated by emm12 (60 isolates, 42.9%), emm1(43, 30.7%), and emm89 (10, 7.1%), and 8 new emm variant subtypes were identified. All strains, invasive or not, harbored the superantigenic genes, speB and slo. The other virulence genes, smeZ, speF, and speC accounted for 96.4, 91.4, and 87.1% of collected isolates, respectively. Further multilocus sequence typing (MLST) placed all strains into 22 individual sequence types (STs), including 4 newly-identified STs (11, 7.9%). All isolates were phenotypically susceptible to penicillin, ampicillin, cefotaxime, and vancomycin, whereas 131(93.5%), 132(94.2%), and 121(86.4%) were resistant to erythromycin, clindamycin, and tetracycline, respectively. Our study highlights high genotypic diversity and high prevalence of macrolide resistance of S. pyogenes among clinical isolates circulating in China.

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Section: Discussionsupporting

confidence: 91%

High Prevalence of Macrolide-resistance and Molecular Characterization of Streptococcus pyogenes Isolates Circulating in China from 2009 to 2016

Fang

Fan

et al. 2017

Front. Microbiol.

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“…Yet another study in Ireland also reported that there were no recognized resistance mechanisms found in GBS isolates resistant to erythromycin [29] . A similar finding was reported from another study in the USA where one isolate showed sensitivity to erythromycin and resistance to clindamycin; however, the isolates did not carry any of ermB , ermTR , or mefA genes [30] . Hence, these findings would deviate the focus of attention from existing knowledge in search of other mechanisms that may be present for reported resistance.…”

Section: Discussionsupporting

confidence: 88%

“…Hence, these findings would deviate the focus of attention from existing knowledge in search of other mechanisms that may be present for reported resistance. A probable mechanism may be a mutation in the 23S rRNA which has been reported in S. pneumoniae for macrolide resistance [30].…”

Section: Discussionmentioning

confidence: 99%

Antibiotic Sensitivity of Group B Streptococcus from Pregnant Mothers and Its Association with Resistance Genes

Dilrukshi¹,

Kottahachchi²,

Dissanayake³

et al. 2023

Med Princ Pract

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Objective This study assessed the antibiotic susceptibility and characterized antibiotic resistance genes of GBS isolates from selected tertiary care hospitals in Western province, Sri Lanka Methods A descriptive cross-sectional study was carried to determine antibiotic sensitivity of GBS among 175 pregnant women of >35 weeks of gestation attending antenatal clinics in four teaching hospitals. Low vaginal and rectal swabs were collected separately, GBS was identified by standard microbiological methods. Antibiotic sensitivity and minimum inhibitory concentration were done according to CLSI guidelines. DNA was extracted from culture isolates and resistance mechanisms were identified by PCR using ermB, ermTR, mefA and linB genes. Results GBS colonization in study sample was 25.7% (45/175) with detection rate of 22.9% and (40/175) and 2.9% (5/175) in vaginal and rectal samples respectively. All isolates were susceptible to penicillin with a MIC range of 0.03-0.12μg/ml. Seventeen (37.7%) were non-susceptible to erythromycin while 6 were intermediate and 11 were resistant. There were 15 (33.3%) non-susceptible isolates for clindamycin with 5 intermediate and ten resistant isolates. Of them seven had inducible clindamycin resistance (iMLSB). Erythromycin and clindamycin MICs ranged 0.03–0.32 μg/ml and 0.06–0.32 μg/ml respectively. The ermB gene was detected in 7(15.5%). The ermTR was found in 16 (35.6%) was significantly associated with iMLSB phenotype (P = 0.005). The mefA gene was detected in two (4.4%) isolates. The linB gene was not detected in tested isolates. Conclusion All isolates were sensitive to penicillin and the most prevalent resistance genotype was ermTR in the study population.

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“…These techniques include the use of chromogenic agar/broth and nucleic acid amplification technology (NAAT) [3, 4]. While both of these techniques have been employed in screening for antenatal GBS colonization, the Centers for Disease Control and Prevention still recommend that culture be performed [5].…”

Section: Introductionmentioning

confidence: 99%

Development of a Novel Test for Simultaneous Bacterial Identification and Antibiotic Susceptibility

Faro

Mitchell

Chen

et al. 2016

Infectious Diseases in Obstetrics and Gynecology

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Background. Elucidation of a pathogen's antimicrobial susceptibility requires subculture after the organism is first isolated. This takes several days, requiring patients to be treated with broad-spectrum antibiotics. This approach contributes to the development of bacterial resistance. Methods. Microtiter wells were coated with a polyclonal antibody targeting the pathogen of interest. Bacterial suspensions were added in the presence/absence of selected antibiotics. After washing, captured bacteria were detected. Findings. Group B streptococcus (GBS), Enterococcus faecalis, and Neisseria gonorrhoeae were each detected at 105 bacteria/mL following a 20-minute incubation period. Susceptibility to select antibiotics was discernable following a 6-hour incubation period (GBS and Enterococcus). Sensitivity was increased to 10−2 bacteria/mL for GBS, 10−1 bacteria/mL for E. faecalis, and 101 bacteria/mL for N. gonorrhoeae following 18–24-hour culture. Conclusion. This novel assay allows for the highly sensitive and specific identification of a pathogen and simultaneous determination of its antimicrobial susceptibility in a reduced time.

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Rapid Determination of Macrolide and Lincosamide Resistance in Group B Streptococcus Isolated from Vaginal-Rectal Swabs

Cited by 10 publications

References 23 publications

High Prevalence of Macrolide-resistance and Molecular Characterization of Streptococcus pyogenes Isolates Circulating in China from 2009 to 2016

High Prevalence of Macrolide-resistance and Molecular Characterization of Streptococcus pyogenes Isolates Circulating in China from 2009 to 2016

Antibiotic Sensitivity of Group B Streptococcus from Pregnant Mothers and Its Association with Resistance Genes

Development of a Novel Test for Simultaneous Bacterial Identification and Antibiotic Susceptibility

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