Rapid determination of seven bioactive components in rat plasma by UPLC―MS/MS and its application to pharmacokinetic compatibility study of Jinlingzi San

Gu, Xiaoting; Wu, Cuiting; Zhang, Mengmeng; Wang, Xin; Liu, You‐Ping; Di, Xin

doi:10.1016/j.jpba.2021.114014

Cited by 9 publications

(4 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A total of 111 of 216 were identified as benzylisoquinoline alkaloids (BIAs). Protoberberine alkaloids, for which most compounds take berberine as the skeleton, include (S)-canadine [ 43 ], tetrahydropalmatine (THP) [ 44 ], scoulerine [ 45 ], corydaline [ 44 ], jatrorrhizine [ 46 ], tetrahydrocolumbamine [ 47 ], cheilanthifoline [ 48 ] and other metabolites ( S1 Table ). Aporphine alkaloids with glaucine as the skeleton include d-glaucine [ 49 ], magnoflorine [ 50 ], corydine [ 51 ], nantenine [ 51 ], thaliporphine [ 52 ], (S)-lirioferine [ 53 ], isocorydine [ 54 ] and others ( S1 Table ).…”

Section: Resultsmentioning

confidence: 99%

Metabolomics and transcriptomics reveal the mechanism of alkaloid synthesis in Corydalis yanhusuo bulbs

Zhao,

Pan,

Tan

et al. 2024

PLoS ONE

View full text Add to dashboard Cite

Corydalis yanhusuo W.T. Wang is a traditional herb. Benzylisoquinoline alkaloids (BIAs) are the main pharmacological active ingredients that play an important role in sedation, relieving pain, promoting blood circulation, and inhibiting cancer cells. However, there are few studies on the biosynthetic pathway of benzylisoquinoline alkaloids in Corydalis yanhusuo, especially on some specific components, such as tetrahydropalmatine. We carried out widely targeted metabolome and transcriptomic analyses to construct the biosynthetic pathway of benzylisoquinoline alkaloids and identified candidate genes. In this study, 702 metabolites were detected, including 216 alkaloids. Protoberberine-type and aporphine-type alkaloids are the main chemical components in C. yanhusuo bulbs. Key genes for benzylisoquinoline alkaloids biosynthesis, including 6-OMT, CNMT, NMCH, BBE, SOMT1, CFS, SPS, STOX, MSH, TNMT and P6H, were successfully identified. There was no significant difference in the content of benzylisoquinoline alkaloids and the expression level of genes between the two suborgans (mother-bulb and son-bulb). The expression levels of BIA genes in the expansion stage (MB-A and SB-A) were significantly higher than those in the maturity stage (MB-C and SB-C), and the content of benzylisoquinoline alkaloids was consistent with the pattern of gene regulation. Five complete single genes were likely to encode the functional enzyme of CoOMT, which participated in tetrahydropalmatine biosynthesis in C. yanhusuo bulbs. These studies provide a strong theoretical basis for the subsequent development of metabolic engineering of benzylisoquinoline alkaloids (especially tetrahydropalmatine) of C. yanhusuo.

show abstract

Section: Resultsmentioning

confidence: 99%

Metabolomics and transcriptomics reveal the mechanism of alkaloid synthesis in Corydalis yanhusuo bulbs

Zhao,

Pan,

Tan

et al. 2024

PLoS ONE

View full text Add to dashboard Cite

show abstract

“…It is worth noting that the DGE in S. officinalis extract was absorbed for a longer time, but the absorption amount was higher than that of the pure compound. The interactions between compounds of herbal extracts in vivo may affect the absorption of target compounds [ 29 ] The reason for the conversion of ZGI to DGE in the extract leads to its increased amount, prolonged absorption time, and increased absorption.…”

Section: Discussionmentioning

confidence: 99%

Simultaneous Determination and Pharmacokinetics Study of Three Triterpenes from Sanguisorba officinalis L. in Rats by UHPLC–MS/MS

Wei

Yang

et al. 2022

Molecules

View full text Add to dashboard Cite

A selective and rapid ultra-high-performance liquid chromatography–tandem mass spectrometry (UHPLC–MS/MS) method was established and validated for the determination of ziyuglycoside I, 3β,19α-dihydroxyurs-12-en-28-oic-acid 28-β-d-glucopyranosyl ester, and pomolic acid in rats after the oral administration of ziyuglycoside I, 3β,19α-dihydroxyurs-12-en-28-oic-acid 28-β-d-glucopyranosyl ester, pomolic acid, and Sanguisorba officinalis L. extract. The separation was carried out on an ACQUITY UPLC®HSS T3 column (2.1 mm × 100 mm, 1.8 μm), using methanol and 5 mmol/L ammonium acetate water as the mobile phase. The three compounds were quantified using the multiple reaction monitoring mode with the electrospray ion source in both the positive and negative mode. Liquid-liquid extraction was applied to the plasma sample preparation. Bifendate was selected as the internal standard. The intra-day and inter-day precision and the accuracy of the method were all within receivable ranges. The lower limit of quantification of ziyuglycoside I, 3β,19α-dihydroxyurs-12-en-28-oic-acid 28-β-d-glucopyranosyl ester, and pomolic acid were 6.50, 5.75, and 2.63 ng/mL, respectively. The extraction recoveries of analytes in rat plasma ranged from 83 to 94%. The three components could be rapidly absorbed into the blood (Tmax, 1.4–1.6 h) both in the single-administration group or S. officinalis extract group, but the first peak of PA occurred at 0.5 h and the second peak at 4–5 h in the S. officinalis extract. Three compounds were eliminated relatively slowly (t1/2, 7.3–11 h). The research was to establish a rapid, sensible, and sensitive UHPLC–MS/MS method using the multi-ion mode for multi-channel simultaneous mensuration pharmacokinetics parameters of three compounds in rats after oral administration of S. officinalis extract. This study found, for the first time, differences in the pharmacokinetic parameters of the three compounds in the monomer compounds and S. officinalis extract administration, which preliminarily revealed the transformation and metabolism of the three compounds in vivo.

show abstract

“…Well-prepared samples help improve the sensitivity and selectivity of analytical methods through the enhancement of separation and detection, besides protecting analytical instruments [18]. An appropriate plasma sample pretreatment should maximize extraction rates, minimize matrix effects, and reduce operating time [19][20][21]. Protein precipitation (PPT), solid-phase extraction (SPE) and LLE, the three most commonly used plasma sample pretreatment methods, were compared in this study [22].…”

Section: Selection Of Extraction Methodsmentioning

confidence: 99%

Simultaneous determination and pharmacokinetics study of three triterpenoid saponins in rat plasma by ultra‐high‐performance liquid chromatography tandem mass‐spectrometry after oral administration of Astragalus Membranaceus leaf extract

Wang,

Borjigin,

Zhang

et al. 2023

J of Separation Science

View full text Add to dashboard Cite

A selective and sensitive ultra‐high‐performance liquid chromatography‐tandem mass spectrometry method was developed and validated for the determination of three triterpenoid saponins isolated from Astragalus membranaceus leaf extract. In this article, a method for simultaneous determination of Huangqiyenin A, Huangqiyenin E, and Huangqiyenin K was established for the first time. The method was successfully applied to the pharmacokinetic study of Astragalus membranaceus leaf extract after oral administration. Liquid–liquid extraction was applied to plasma sample preparation. Multiple reaction monitoring mode with an electrospray ion source in positive electrospray ionization was chosen to quantify the analytes. Chromatographic separation was performed on a Waters HSS T3 column, using gradient elution with a mobile phase composed of acetonitrile and 5 mM ammonium acetate/water. The pharmacokinetic results showed that all three compounds had the characteristics of rapid absorption‐slow metabolism trend. The time of maximum plasma concentration of Huangqiyenin A is higher than Huangqiyenin E and Huangqiyenin K. And the maximum plasma concentration of Huangqiyenin A is higher as well.The pharmacokinetic results revealed the pharmacokinetic characteristics of the three analytes in rat plasma, which could provide a helpful reference for the further study of Astragalus membranaceus leaf extract.

show abstract

Rapid determination of seven bioactive components in rat plasma by UPLC―MS/MS and its application to pharmacokinetic compatibility study of Jinlingzi San

Cited by 9 publications

References 21 publications

Metabolomics and transcriptomics reveal the mechanism of alkaloid synthesis in Corydalis yanhusuo bulbs

Metabolomics and transcriptomics reveal the mechanism of alkaloid synthesis in Corydalis yanhusuo bulbs

Simultaneous Determination and Pharmacokinetics Study of Three Triterpenes from Sanguisorba officinalis L. in Rats by UHPLC–MS/MS

Simultaneous determination and pharmacokinetics study of three triterpenoid saponins in rat plasma by ultra‐high‐performance liquid chromatography tandem mass‐spectrometry after oral administration of Astragalus Membranaceus leaf extract

Contact Info

Product

Resources

About