Rapid determination of the neurotoxin lolitrem B in perennial ryegrass by high-performance liquid chromatography with fluorescence detection

Gallagher, Rex T.; Hawkes, Allan D.; Stewart, Jan M.

doi:10.1016/s0021-9673(01)90438-8

Cited by 93 publications

(62 citation statements)

References 9 publications

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“…The general pattern of lolitrem B concentrations was similar to that reported by Gallagher et al (1985Gallagher et al ( , 1986) and di Menna et al (1992) in which values were higher in leaf sheath than in leaf blade, and also higher in "outer" than in "inner" leaf sheath components. Our results show that there is little variation in lolitrem B concentrations within either LI or L2, and that large concentration gradients are present within the older leaves, L3 and L4.…”

Section: Discussionsupporting

confidence: 88%

“…This difference may, however, only reflect differences in the composition of the material designated to leaf sheath categories. Our results refer to leaves of different ages within ryegrass tillers, whereas those of Gallagher et al (1985) refer to a more general division of plants into leaf sheath/stem above and below a 1-inch level, as well as separation of the leaf blade.…”

Section: Discussionmentioning

confidence: 69%

“…Our results show that there is little variation in lolitrem B concentrations within either LI or L2, and that large concentration gradients are present within the older leaves, L3 and L4. Moreover, within L3 and L4, the maximum concentrations were in the upper rather than in the basal portion of leaf sheath-a result which apparently differs from that of Gallagher et al (1985). This difference may, however, only reflect differences in the composition of the material designated to leaf sheath categories.…”

Section: Discussionmentioning

confidence: 78%

“…Concentrations were greater in the outer leaf sheath than the inner leaf sheath or leaf blade. Concentrations were also greater in basal sheath/ stem tissue than in that of the upper sheath/stem or leaf blade (Gallagher et al 1985;di Menna et al 1992). A. lolii concentrations were not reported.…”

Section: Introductionmentioning

confidence: 81%

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Distributions of the fungal endophyteAcremonium lolii,and of the alkaloids lolitrem B and peramine, within perennial ryegrass

Keogh¹,

Tapper²,

Fletcher³

1996

New Zealand Journal of Agricultural Research

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Ryegrass tillers, from a ryegrass staggers-prone pasture, were dissected into constituent live leaves of different ages. Leaves were further divided into three blade and two sheath portions. Acremonium lolii, lolitrem B, and peramine concentrations were determined for each defined portion. The mean % distribution and concentrations of A. lolii and lolitrem B in the sheath were much higher, and the mean % distribution of peramine in the sheath was much lower, than in the blade portions. Within leaves, both the % distribution and the concentration of A. lolii increased progressively from blade tip to lower sheath; the patterns for % distribution and concentration of lolitrem B were similar to those for A. lolii, except that lower sheath values were less than those of upper sheath portions; the % distribution of peramine was higher in the lower blade portions than elsewhere, and peramine concentrations were higher in portions adjacent to the ligule. Within tillers, A. lolii concentrations were lower in the youngest and oldest leaves; peramine concentrations were lower for older than for younger leaves; and lolitrem B concentrations increased progressively from youngest to oldest leaves.

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Section: Discussionsupporting

confidence: 88%

Section: Discussionmentioning

confidence: 69%

Section: Discussionmentioning

confidence: 78%

Section: Introductionmentioning

confidence: 81%

See 2 more Smart Citations

Distributions of the fungal endophyteAcremonium lolii,and of the alkaloids lolitrem B and peramine, within perennial ryegrass

Keogh¹,

Tapper²,

Fletcher³

1996

New Zealand Journal of Agricultural Research

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“…Samples were analysed for ergovaline by a modification of the method of Rottinghaus et al (1991). Samples of perennial ryegrass were analysed for lolitrem B by a modification of the method of Gallagher et al (1985). Alkaloid concentrations were expressed as ppm (mg alkaloid/kg dry matter).…”

Section: Chemical Analysismentioning

confidence: 99%

Ergovaline concentration in perennial ryegrass infected with a lolitrem B‐free fungal endophyte in north‐west Spain

Oliveira¹,

Rottinghaus

González³

2003

New Zealand Journal of Agricultural Research

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Ergovaline concentrations in two perennial ryegrass genotypes (EI 19 and EI24) infected with one lolitrem B-free fungal endophyte were determined over a 3-year period at two different locations in north-west Spain. There was a significant sampling date effect over 3 years and two locations (P < 0.05). The mean ergovaline concentrations ranged from 0.06 ppm in early spring to 0.57 ppm in June. EI19 genotype had significantly (P < 0.05) higher ergovaline concentrations (0.37 ppm, SE = 0.09) than EI24 genotype (0.33 ppm, SE = 0.08) only at one location in year 2000. Under north-west Spanish conditions, ergovaline levels obtained in this study were not so high to reach values potentially toxic for animals. The results presented in this paper suggest that agriculturally useful ryegrass endophyte associations can be developed free from lolitrem B, and which do not accumulate excessive concentrations of ergovaline.

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Qualitative and quantitative analysis of endophyte alkaloids in perennial ryegrass using near‐infrared spectroscopy

et al. 2017

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Rapid determination of the neurotoxin lolitrem B in perennial ryegrass by high-performance liquid chromatography with fluorescence detection

Cited by 93 publications

References 9 publications

Distributions of the fungal endophyteAcremonium lolii,and of the alkaloids lolitrem B and peramine, within perennial ryegrass

Distributions of the fungal endophyteAcremonium lolii,and of the alkaloids lolitrem B and peramine, within perennial ryegrass

Ergovaline concentration in perennial ryegrass infected with a lolitrem B‐free fungal endophyte in north‐west Spain

Qualitative and quantitative analysis of endophyte alkaloids in perennial ryegrass using near‐infrared spectroscopy

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