Rapid determination of valsartan in human plasma by protein precipitation and high-performance liquid chromatography

Máček, Ján; Klima, Josef; Ptáček, Pavel

doi:10.1016/j.jchromb.2005.12.035

Cited by 84 publications

(63 citation statements)

References 9 publications

(14 reference statements)

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“…Numerous high performance liquid chromatography (HPLC) methods attached with fluorimetric detector have been reported for the estimation of valsartan in human plasma [5][6][7][8] . Similarly, several HPLC methods using tandem mass spectroscopy (LC/MS/ MS) have also been reported for analysis of drug in plasma with their high sensitivity [9][10][11] .…”

Section: Several Bioanalytical Methods Have Been Reportedmentioning

confidence: 99%

“…It is also necessary that the extraction process be simpler as rapid as possible. Although, the extraction process has been well established, it is affected by the choice of deproteinizing agent used, volume of the extracting solvent, simplicity of the extraction and the protein separating process [1,7,8,17] . Furthermore, in the estimation process, short analysis time, sensitivity, robustness, precision, accuracy, sharpness of the peak and other economic aspects should be considered.…”

Section: Several Bioanalytical Methods Have Been Reportedmentioning

confidence: 99%

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Statistical Optimization of Extraction Process for the Quantification of Valsartan in Rabbit Plasma by a HPLC Method

Reddy¹

2017

ijps

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This article describes a high performance liquid chromatography method for determination of valsartan in rabbit plasma. The method was statistically optimized, developed and validated as per United States of Food and Drug Administration guidelines. The solvent deproteination technique was opted for the extraction of valsartan from rabbit plasma. Full factorial design was used for the optimization of an extraction method. The main effect of deproteinating agent, volume of deproteinating agent, speed of centrifugation and time of centrifugation was found to be significant at P<0.0001 on all the responses. After deproteinization, the drug was analysed on a C 18 column using 20 mM ammonium formate:acetonitrile (58:42 v/v) as the mobile phase with a flow rate of 0.9 ml/min. The standard calibration curve was constructed in the concentration range of 75 ng/ml to 4.0 µg/ml and linearity was found to be 0.9989. Losartan was used as the internal standard. The retention time of valsartan and the internal standard was found to be 11.394 and 6.343 min, respectively. No interference peak was perceived. From the accuracy results, the relative error was found between 0.99 and 13.01% and relative standard deviation was between 1.43 and 12.19%. The percent relative standard deviation of intra-day and inter-day precision was found to be less than 15%. Limit of detection and limit of quantitation were found to be 22.00 and 66.67 ng/ml, respectively. From pharmacokinetic applications, the peak plasma concentration (C max ) of valsartan oral suspension was found to be 1092.67±39.62 ng/ml at 0.67±1.24 h. The half-life and area under the curve were found to be 19.92±10.28 h and 8393.35±131.14 ng/ml, respectively. The high performance liquid chromatography method was successfully demonstrated as rapid and sensitive method which can be used as an alternative for the analysis of valsartan in plasma samples.

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Section: Several Bioanalytical Methods Have Been Reportedmentioning

confidence: 99%

Section: Several Bioanalytical Methods Have Been Reportedmentioning

confidence: 99%

Statistical Optimization of Extraction Process for the Quantification of Valsartan in Rabbit Plasma by a HPLC Method

Reddy¹

2017

ijps

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“…Few analytical methods have been developed for the determination of Sacubitril and Valsartan individually and in combination with other drugs. [8][9][10][11][12][13][14][15] So we have developed a novel, simple and highly sensitive UV spectrophotometric methods for assay of Sacubitril and valsartan in bulk and pharmaceutical formulations and validated according to ICH guidelines.…”

Section: Introductionmentioning

confidence: 99%

Stability Indicating Rp-HPLC Method for Simultaneous Estimation of Sacubitril and Valsartan in Bulk and Combined Pharmaceutical Dosage Form

Ahmed¹

2017

WJPPS

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“…Valsartan is chemically N-(1-oxopentyl)-N-[[2'-(1Htetrazol-5-yl)[1,1'-biphenyl] -4-yl]methyl]-Lvaline. [1][2][3][4] Methods such as HPLC [5][6][7] , LC-MS [8][9] , protein precipitation [10] and simultaneous UV-spectrophotometric methods [11][12] are reported for estimation of valsartan alone or in combination with other agents. Sacubitril is chemically 4-…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Estimation of Sacubitril and Valsartan in Pharmaceutical Dosage Form by Development and Validation of Stability Indicating Chromatographic Method

Patel¹

2017

WJPPS

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This study aims to develop and validate a stability indicating HPLC method for simultaneous estimation of sacubitril and valsartan in pharmaceutical dosage form. Sacubitril and valsartan separation was achieved by LC-20 AT C 18 (250mm x 4.6 mm) column and buffer (potassium phosphate, pH 3.0): methanol (50:50) as mobile phase, at a flow rate of 1 ml/min (milliliter per minute). Detection was carried out at 224 nm (nanometer). The different HPLC experimental parameters were optimized and the method was validated according to standard guideline. Forced degradation experiments were carried out by exposing sacubitril and valsartan standard and sample for thermal, photolytic, oxidative and acid-base hydrolytic stress conditions. Retention time of sacubitril and valsartan were found to be 4.170 min (minute) and 6.530 min (minute) respectively. The method has been validated for linearity, accuracy, precision, LOD, and LOQ. Linearity observed for sacubitril is 12.25-36.75 μg/ml (microgram per milliliter) and for valsartan is 12.75-38.25 μg/ml (microgram per milliliter).The results showed that sacubitril and valsartan and the other degradation products were fully resolved and thus the proposed method is stability-indicating.

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Rapid determination of valsartan in human plasma by protein precipitation and high-performance liquid chromatography

Cited by 84 publications

References 9 publications

Statistical Optimization of Extraction Process for the Quantification of Valsartan in Rabbit Plasma by a HPLC Method

Statistical Optimization of Extraction Process for the Quantification of Valsartan in Rabbit Plasma by a HPLC Method

Stability Indicating Rp-HPLC Method for Simultaneous Estimation of Sacubitril and Valsartan in Bulk and Combined Pharmaceutical Dosage Form

Simultaneous Estimation of Sacubitril and Valsartan in Pharmaceutical Dosage Form by Development and Validation of Stability Indicating Chromatographic Method

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