Rapid diagnosis of Brucella melitensis in blood: some operational characteristics of the BACT/ALERT

Solomon, Harvey M.; Jackson, Daren C.

doi:10.1128/jcm.30.1.222-224.1992

Cited by 44 publications

(28 citation statements)

References 6 publications

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“…In our own experience, the magnitude of Brucella bacteremia as determined by the Isolator Microbial Tube system in children with acute infection is extremely variable, ranging from 1.3 to Ͼ1,000 CFU/ml, with a median of 88 CFU/ml (34). Time-todetection correlated inversely with the concentration of viable organisms in the blood sample, validating the results of experimental studies (30,35).…”

Section: Broth Culture Methodssupporting

confidence: 75%

“…However, the key explanation for the delayed detection of the organism by some automated blood culture systems appears to be the slow release of CO 2 by members of the genus. In a series of in vitro studies with the BacT/Alert system (Organon Teknika Corporation, Durham, N.C.), a slow production of CO 2 by B. melitensis compared to Escherichia coli and Staphylococcus aureus was demonstrated, and the peak values obtained were of lower magnitude (30). In a study by Gamazo et al, broth culture media showed visible turbidity, indicating a large bacterial concentration, on average 24 h earlier than detection of positivity by the BACTEC 730 instrument (10).…”

Section: Broth Culture Methodsmentioning

confidence: 99%

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Detection of Brucellae in Blood Cultures

Yagupsky

1999

J Clin Microbiol

230

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Section: Broth Culture Methodssupporting

confidence: 75%

Section: Broth Culture Methodsmentioning

confidence: 99%

Detection of Brucellae in Blood Cultures

Yagupsky

1999

J Clin Microbiol

230

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“…Additional, albeit uncontrolled, observations have been published regarding the performance characteristics of BacT/ Alert. The system has been used to recover both Brucella melitensis (167) and another Brucella isolate (204a) from standard aerobic bottles. The time to detection of microbial growth, less than 3 days, was similar for both isolates.…”

Section: Blood Culture Systemsmentioning

confidence: 99%

Update on detection of bacteremia and fungemia

1997

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The presence of microorganisms in a patient's blood is a critical determinant of the severity of the patient's illness. Equally important, the laboratory isolation and identification of a microorganism present in blood determine the etiologic agent of infection, especially when the site of infection is localized and difficult to access. This review addresses the pathophysiology and clinical characteristics of bacteremia, fungemia, and sepsis; diagnostic strategies and critical factors in the detection of positive blood cultures; characteristics of manual and instrument approaches to bacteremia detection; approaches for isolating specific microorganisms associated with positive blood cultures; and rapid methods for the identification of microorganisms in blood cultures.

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“…Solomon et al. [9] demonstrated a mean detection time of 48 ± 1 h in eight of 10 replicates seeded with a stock containing 10 2 CFU/mL of B. melitensis by using BacT/Alert StAe bottles. Zimmermann et al.…”

Section: Discussionmentioning

confidence: 99%

Brucella detection in blood: comparison of the BacT/Alert standard aerobic bottle, BacT/Alert FAN aerobic bottle and BacT/Alert enhanced FAN aerobic bottle in simulated blood culture

Sümerkan

Gökahmetoğlu

Eşel

2001

Clinical Microbiology and Infection

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The objective of this study was to compare the performances of the standard aerobic bottle (StAe), FAN aerobic (FANAe) and enhanced FAN aerobic (E-FANAe) (the charcoal component of the FANAe was revised recently to improve the feasibility of Gram smear interpretation) blood culture bottles for BacT/Alert system for the detection of Brucella melitensis in simulated blood culture. Triplicate strains of eight clinical isolates of B. melitensis were studied. Each bottle was inoculated with 5 mL of freshly collected human blood at three different targeted bacterial inocula (10(1), 10(2) and 10(3) CFU/bottle). All bottles were monitored for up to 21 days or until they became positive. The results of time to detection (TTD) on the eight B. melitensis samples were as follows: at 10(1) CFU/bottle, the E-FANAe had a mean TTD significantly shorter than the StAe (48 h vs. 56.2 h, P < 0.05); and at 10(3) CFU/bottle, the FANAe and E-FANAe had a mean TTD significantly shorter than the StAe (41.2 h and 40 h vs. 45.6 h, P < 0.05). The reproducibilities (no.of positive signals/no.of all bottles) of three bottle systems were as follows: at 10(1) CFU/bottle, the reproducibilities of StAe, FANAe and E-FANAe were 96, 83 and 58%, respectively. At 10(3) CFU/bottle, the reproducibilities of StAe, FANAe and E-FANAe were 95, 95 and 91%, respectively. Positive results for the presence of bacteria in Gram smears were confirmed in 68% of StAe, 54% of FANAe and 90% of E-FANAe. In case of suspected brucellosis, the combination of one StAe bottle and one E-FANAe bottle seems to provide the highest and fastest recovery of the organism.

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Rapid diagnosis of Brucella melitensis in blood: some operational characteristics of the BACT/ALERT

Cited by 44 publications

References 6 publications

Detection of Brucellae in Blood Cultures

Detection of Brucellae in Blood Cultures

Update on detection of bacteremia and fungemia

Brucella detection in blood: comparison of the BacT/Alert standard aerobic bottle, BacT/Alert FAN aerobic bottle and BacT/Alert enhanced FAN aerobic bottle in simulated blood culture

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