Rapid Diagnosis of Campylobacter Enteritis

Sazie, Elizabeth

doi:10.7326/0003-4819-96-1-62

Cited by 35 publications

(15 citation statements)

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“…En los laboratorios en que se realiza de rutina la detección de Campylobacter, el cultivo en medio selectivos en microaerofilia e identificación posterior por métodos fenotípicos es el método habitual. Debido al costo y demora del cultivo de Campylobacter, desde hace algunos años han surgido estrategias rápidas y de bajo costo para el diagnóstico precoz de éste patóge-no, como la búsqueda directa con microscopia de contraste de fase y tinción de Gram, con sensibilidad de 43,5 a 65,5% y especificidad de 95 a 99,4% 23,24 ; un estudio más reciente arrojó una sensibilidad de 89%, especificidad de 99,7% y valor predictor positivo de 97% 25 . Otras tinciones desarrolladas, como la tinción con fucsina básica 1%…”

Section: Discussionunclassified

Evaluación de la tinción de Hucker para la búsqueda rutinaria de Campylobacter sp en el estudio de un síndrome diarreico agudo

et al. 2005

Rev. chil. infectol.

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Section: Discussionunclassified

Evaluación de la tinción de Hucker para la búsqueda rutinaria de Campylobacter sp en el estudio de un síndrome diarreico agudo

et al. 2005

Rev. chil. infectol.

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“…However, due to their thin morphology, campylobacters cannot be readily visualized with the safranin counterstain that is used in most Gram stain procedures. Instead, carbol fuchsin or 0.1% aqueous basic fuchsin can be substituted as the counterstain to achieve visualization of the organism in direct smears of stool (22,25,33). Evaluation of this direct microscopic method in stool specimens collected from patients with acute diarrheal disease shows a sensitivity ranging from 66% to 94% and a specificity of Ͼ95% (22,25,33).…”

Section: Discussionmentioning

confidence: 99%

“…Some of these involve the direct microscopic detection of the microorganism in stool, the recovery of the organism from culture following the use of a filtration method, or the use of a specialized selective medium for the enhanced recovery of Campylobacter from stool (9). Most clinical laboratories do not use the direct microscopic or filtration method, because microscopy is insensitive (22,25,33), and filtration is cumbersome and may lack sensitivity (11). The use of a selective medium is recommended for the optimal recovery of Campylobacter from stool samples (9).…”

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confidence: 99%

Comparison of Premier CAMPY Enzyme Immunoassay (EIA), ProSpecT Campylobacter EIA, and ImmunoCard STAT! CAMPY Tests with Culture for Laboratory Diagnosis of Campylobacter Enteric Infections

et al. 2010

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Campylobacter enteritis is a food-borne or waterborne illness caused almost exclusively by Campylobacter jejuni and, to a lesser extent, by Campylobacter coli. These organisms produce indistinguishable clinical diseases and together represent the second most common cause of bacterial diarrhea in the United States and the leading cause of enteric infection throughout the world. The conventional approach to the laboratory diagnosis of Campylobacter enteritis is based on the recovery of the organism from a stool specimen, which requires the use of a specialized medium incubated at 42°C for several days in an artificially created microaerophilic environment. Recently, several commercially available enzyme immunoassays (EIAs) have been developed for the direct detection of C. jejuni and C. coli in stool specimens. This study compared conventional culture with three EIA methods, the Premier CAMPY EIA (Meridian Bioscience, Cincinnati, OH), the ProSpecT Campylobacter EIA (Remel, Lenexa, KS), and the ImmunoCard STAT! CAMPY test (Meridian Bioscience, Cincinnati, OH), for the detection of C. jejuni and C. coli in 485 patient stool samples. Discordant results were arbitrated by using an in-house, real-time PCR assay that was developed and validated by a public health reference laboratory. Following analyses of the discrepant specimens by PCR, the sensitivity and specificity of both the Premier CAMPY and ProSpecT Campylobacter EIAs were 99.3% and 98%, respectively, while the ImmunoCard STAT! CAMPY test had a sensitivity of 98.5% and a specificity of 98.2%. By use of the PCR test as the reference standard, culture detected 127 of 135 Campylobacter-positive stool specimens, yielding a sensitivity of 94.1%. These results showed that the three EIAs evaluated in this study provide a rapid and reliable alternative for the laboratory diagnosis of enteric infections with C. jejuni and C. coli and that conventional culture may no longer be recognized as the "gold standard" for diagnosis.

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“…The slide smear of the fresh stool was made on new slide; it was air dried and heat fixed. The Gram stain was done using conventional gram stain procedures with 0.3% Carbol-fuschin (Sigma-Aldrich) as counter stain for five minutes (Sazie & Titus, 1982). Mucous part of the stool was inoculated on blood free charcoal based selective media (CCDA) and blood contained media selective for campylobacter (BioMerieux, Paris, France).…”

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confidence: 99%

Role of microscopic examination of stool specimens in the diagnosis of campylobacter infection from children with acute diarrhoea in Kampala, Uganda

Mshana

Joloba

et al. 2010

Tanzania J Hlth Res

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Abstract:Campylobacter species are a frequent cause of enteritis and less often of extraintestinal infections in humans. The diagnosis of campylobacter infection depends mainly on culture which is difficult and expensive to be done as routine in most clinical microbiology laboratories in the developing countries. This study was conducted to determine the sensitivity and specificity of Gramstain of the stool in diagnosis of campylobacter infection, using culture as the gold standard. A total of 226 stool specimens were obtained from children with acute diarrhoea, attending Mulago Hospital in Kampala, Uganda. Stool smears were made and conventional Gram stain done using 0.3% carbolfuschin as counter stain for 5 minutes. Mucous part of the stool was cultured in Charcoal Ceferaperazone Deoxycholate Agar and blood contained selective media. A total of 21 stool samples (9.3%) were positive by culture and 17 (7.5%) by Gram stain. Sensitivity and specificity of Gram stain in the diagnosis of campylobacter infection was 76% and 99.5%, respectively with positive predictive value of 94.1%. A total of 127 (56.2%) had white blood cells (WBC) in stool and there was strong association between WBC in stool and the presence of campylobacter infection (P=0.001). Gram stain is a good alternative in diagnosis of campylobacter infection in place where facilities for culture are limited. ________________________________________________________________________

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Rapid Diagnosis of Campylobacter Enteritis

Cited by 35 publications

References 3 publications

Evaluación de la tinción de Hucker para la búsqueda rutinaria de Campylobacter sp en el estudio de un síndrome diarreico agudo

Evaluación de la tinción de Hucker para la búsqueda rutinaria de Campylobacter sp en el estudio de un síndrome diarreico agudo

Comparison of Premier CAMPY Enzyme Immunoassay (EIA), ProSpecT Campylobacter EIA, and ImmunoCard STAT! CAMPY Tests with Culture for Laboratory Diagnosis of Campylobacter Enteric Infections

Role of microscopic examination of stool specimens in the diagnosis of campylobacter infection from children with acute diarrhoea in Kampala, Uganda

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