Rapid diagnosis of red sea bream iridovirus infection using the polymerase chain reaction

Oshima, Syun-ichirou; Hata, Jun-ichiro; Hirasawa, Noritaka; Ohtaka, Taro; Hirono, Ikuo; Aoki, Takashi; Yamashita, Shinya

doi:10.3354/dao032087

Cited by 52 publications

(33 citation statements)

References 5 publications

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“…In addition to taxonomic divisions based on the sequence of the MCP gene, other regions of the iridovirus genome have also been used. Thus, sensitive PCR tests have been developed to detect iridovirus infections in amphibians (Gould et al 1995) and in marine fish infected with red sea bream iridovirus (Kurita et al 1998, Oshima et al 1998. Recently, Hyatt et al (2000) used approaches similar to ours to analyze a panel of established and emerging iridovirus pathogens.…”

Section: Discussionmentioning

confidence: 99%

Characterization of an iridovirus from the cultured pig frog Rana grylio with lethal syndrome

Qy¹,

Xiao²,

Zq³

et al. 2001

Dis. Aquat. Org.

124

102

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Three virus isolates, RGV-9506, RGV-9807 and RGV-9808, were obtained from cultured pig frogs Rana grylio undergoing lethal infections. Previously, the first isolate, RGV-9506, was shown to be an iridovirus based on ultrastructural and morphological studies. In the present study, the original isolate, along with 2 recent ones, were more extensively characterized by experimental infection studies, histopathology, electron microscopy, serological reactivity, gel electrophoresis of viral polypeptides and DNA restriction fragments, PCR amplification, and nucleic acid sequence analysis of the major capsid protein (MCP) gene. The 3 isolates were shown to be identical to each other, and very similar to FV3, the type species of the genus Ranavirus (family Iridoviridae). These results suggest that RGV should be considered a strain of FV3, and indicate that FV3-like iridoviruses are capable of causing widespread, severe disease among cultured frogs. KEY WORDS: Rana grylio · Iridovirus · FV3 · Ranavirus · Viral disease Resale or republication not permitted without written consent of the publisherDis Aquat Org 48: [27][28][29][30][31][32][33][34][35][36] 2001 atic, and renal hematopoietic tissues. Moreover, although infection of redfin perch leads to high levels of mortality, EHNV infects other fish species with varying results (Langdon et al. 1986). In another example, localized die-offs of tiger salamanders Ambystoma tigrinum and largemouth bass Micropterus salmoides in North America have been linked to the presence of novel iridoviruses (Plumb et al. 1996, Jancovich et al. 1997, Bollinger et al. 1999. Finally, iridoviruses can infect not only different species within the same taxonomic class, but the same virus can infect animals from different classes (Mao et al. 1999a). Thus, an iridovirus isolated from the ornate burrowing frog Bohle iridovirus has been shown to cause mortality in barramundi fish following experimental infections (Moody & Owens 1994). The above observations indicate that iridoviruses infecting aquatic animals enjoy a worldwide distribution and are increasingly associated with serious disease (Ahne et al. 1997.Although some aspects of iridovirus biology have been examined (Willis et al. 1985, Hedrick et al. 1992, Zupanovic et al. 1998a,b, Bollinger et al. 1999, Mao et al. 1999a, additional work is needed to understand the ecology, epidemiology, and pathogenicity of iridovirus-host interaction. In this study, 3 RGV isolates linked to severe disease in cultured frogs were characterized by experimental infection, histopathology, electron microscopy, serological cross reactivity, gel electrophoresis of virion polypeptides and DNA restriction fragments, PCR amplification, and sequence analysis of the major capsid protein (MCP) gene. We found that the 3 isolates were essentially identical to each other and closely related to FV3. In view of this, it is likely that RGV is a strain of FV3. MATERIALS AND METHODSPreparation of RGV isolates. RGV isolates were prepared from tissues (liver, kidney and s...

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Section: Discussionmentioning

confidence: 99%

Characterization of an iridovirus from the cultured pig frog Rana grylio with lethal syndrome

Qy¹,

Xiao²,

Zq³

et al. 2001

Dis. Aquat. Org.

124

102

View full text Add to dashboard Cite

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“…Interestingly, the highest MCP gene copy number was obtained in the heart while the lowest copy number was detected in the blood. Oshima et al (1998) did not obtain an amplified product in the blood of infected red seabream using con ventional PCR, and they suggested that RSIV might be present at low levels in the blood during the early stages of infection. We obtained similar results using the same method.…”

Section: Al 2001)mentioning

confidence: 99%

“…Recently, the use of PCR for rapid diagnosis of RSIV prior to the onset of infection has been developed (Oshima et al, 1996;Kurita et al, 1998;Oshima et al, 1998). Although conventional PCR can detect viruses in the infected organisms (Mackay et al, 2002), it is not sufficiently sensitive for quantifying the number of viral particles (Dhar et al, 2001).…”

mentioning

confidence: 99%

Development of a Real-time PCR Assay for the Detection and Quantification of Red Seabream Iridovirus (RSIV).

Caipang¹,

Hirono²,

Aoki³

2003

Fish Pathol.

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“…A recent survey showed that 25 species of fish are susceptible EHNV, SFIV and GIV to red sea bream has not been demonstrated . Diagnostic methods such as an IF test and polymerase chain reaction amplification were reported , Kurita et al 1998, Oshima et al 1998). However, control measures for this disease have not been established.…”

mentioning

confidence: 99%

Effectiveness of a vaccine against red sea bream iridoviral disease in a field trial test

Nakajima¹,

Maeno²,

Honda³

et al. 1999

Dis. Aquat. Org.

109

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Rapid diagnosis of red sea bream iridovirus infection using the polymerase chain reaction

Cited by 52 publications

References 5 publications

Characterization of an iridovirus from the cultured pig frog Rana grylio with lethal syndrome

Characterization of an iridovirus from the cultured pig frog Rana grylio with lethal syndrome

Development of a Real-time PCR Assay for the Detection and Quantification of Red Seabream Iridovirus (RSIV).

Effectiveness of a vaccine against red sea bream iridoviral disease in a field trial test

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