Rapid Diagnosis of Tick-borne Encephalitis by means of Enzyme Linked Immunosorbent Assay

Hofmann, H; Frisch-Niggemeyer, W; Heinz, Franz X.

doi:10.1099/0022-1317-42-3-505

Cited by 52 publications

(15 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…A recent infection with an in¯uenza A virus was indicated by a four-fold increase of complement-®xing antibodies and, in cases with only one serum sample available, by the presence of high levels of in¯uenza A virus-speci®c antibodies ( b 1:64). The detection of tick borne encephalitis virus infections was based on the detection of virus-speci®c IgM antibodies in serum and CSF [Hofmann et al, 1979].…”

Section: Methodsmentioning

confidence: 99%

Screening for possible failure of herpes simplex virus PCR in cerebrospinal fluid for the diagnosis of herpes simplex encephalitis

Puchhammer‐Stöckl

Presterl

Croÿ

et al. 2001

Journal of Medical Virology

View full text Add to dashboard Cite

The objectives of this study were to evaluate the reliability of herpes simplex virus (HSV) PCR testing in cerebrospinal fluid (CSF) for the detection of herpes simplex encephalitis. This was done by examining retrospectively the clinical follow-up of a large group of patients tested routinely by HSV-PCR. In addition, an attempt was made to assess the incidence of herpes simplex encephalitis in a central European population. CSF samples from 1,427 patients from all Vienna hospitals were submitted for HSV-PCR testing during a period of 4 years and 8 months. Herpes simplex encephalitis was detected by PCR in 12 cases and by serological methods in one additional patient. Retrospective analysis of the course of disease, which was possible in 799 PCR-negative patients, led to the identification of three additional cases in which herpes simplex encephalitis appears to have occurred despite negative PCR results. Failure of the PCR in these patients is most likely due to the time of obtaining CSF during the course of disease. A high specificity of the assay was demonstrated by the lack of false positive results in any of the 708 cases in which other causes for the neurological symptoms had been identified in the follow-up. The incidence of herpes simplex encephalitis in the population of Vienna was between 1 case/469,000-577,000 individuals/year. The highest annual incidence was detected in the age group between 3 months and 3 years, which, however, could not be confirmed statistically.

show abstract

Section: Methodsmentioning

confidence: 99%

Screening for possible failure of herpes simplex virus PCR in cerebrospinal fluid for the diagnosis of herpes simplex encephalitis

Puchhammer‐Stöckl

Presterl

Croÿ

et al. 2001

Journal of Medical Virology

View full text Add to dashboard Cite

show abstract

“…The detection of tickborne encephalitis virus (TBEV) infection was based on the presence of virus-specific IgM antibodies in serum and CSF specimens [13].…”

Section: Patient Population During the Periods Of Epidemics Of Influmentioning

confidence: 99%

Acute Encephalopathy Associated with Influenza A Virus Infection

Steininger

Popow‐Kraupp

Laferl

et al. 2003

Clinical Infectious Diseases

108

View full text Add to dashboard Cite

Twenty-one patients aged 4-78 years with influenza A virus-associated acute encephalopathy were studied. Influenza A virus could be detected only in a cerebrospinal fluid (CSF) specimen obtained from 1 of 18 patients, despite the use of a highly sensitive polymerase chain reaction assay. Six patients experienced influenzal encephalopathy during the course of respiratory illness. Five of these patients had hypoprothrombinemia and 4 had increased serum creatinine levels, indicating hepatic and/or renal dysfunction. Fourteen patients experienced postinfluenzal encephalopathy

show abstract

“…Arbovirus serology should benefit from the development of an ELISA. ELISAs have been investigated for use with some alphaviruses and flaviviruses, and previous studies indicate that the ELISA is sensitive in detecting virus-specific antibody and is directly comparable to the standard serological techniques (Dittmar et al, 1979;Frazier & Shope, 1979;Hoffmann et al, 1979;Roehrig et al, 1980); however, these investigators did not analyse the formidable problem of serological cross-reactivity demonstrable by standard serology among the vector-borne togaviruses. While production of pure virus antigen is quite feasible for alphaviruses, production of comparable amounts of flavivirus antigen is impractical.…”

Section: Discussionmentioning

confidence: 99%

Development of an Enzyme-linked Immunosorbent Assay for the Identification of Arthropod-borne Togavirus Antibodies

Roehrig

1982

Journal of General Virology

View full text Add to dashboard Cite

Rapid Diagnosis of Tick-borne Encephalitis by means of Enzyme Linked Immunosorbent Assay

Cited by 52 publications

References 9 publications

Screening for possible failure of herpes simplex virus PCR in cerebrospinal fluid for the diagnosis of herpes simplex encephalitis

Screening for possible failure of herpes simplex virus PCR in cerebrospinal fluid for the diagnosis of herpes simplex encephalitis

Acute Encephalopathy Associated with Influenza A Virus Infection

Development of an Enzyme-linked Immunosorbent Assay for the Identification of Arthropod-borne Togavirus Antibodies

Contact Info

Product

Resources

About