Rapid Differentiation of Filariae in Unstained and Stained Paraffin-Embedded Sections by a High-Resolution Melting Analysis PCR Assay

Wongkamchai, Sirichit; Mayoon, Benjaporn; Kanakul, Namthip; Foongladda, Suporn; Wanachiwanawin, Darawan; Nochote, Hathai; Loymek, Sumart

doi:10.1089/vbz.2014.1762

Cited by 6 publications

(2 citation statements)

References 23 publications

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“…Furthermore, assays can be performed on a large number of samples simultaneously, with only very small sample volumes needed for a single analysis [ 108 ]. Additionally, reactions can be performed on paraffin-embedded samples, thus providing an effective alternative diagnostic procedure [ 109 ].…”

Section: Next-generation Methods For Filariasis Diagnosticsmentioning

confidence: 99%

Beyond Tradition: Exploring Cutting-Edge Approaches for Accurate Diagnosis of Human Filariasis

Pietrzak,

Łuczak,

Wiśniewski

2024

Pathogens

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Filariasis is recognised as a global public health threat, particularly in tropical and subtropical regions. It is caused by infection with a nematode parasite of the superfamily Filarioidea, including Wuchereria bancrofti, Brugia malayi, Onchocerca volvulus, and Onchocerca lupi. Three main types of filariasis have been classified: lymphatic filariasis, subcutaneous filariasis, and serous cavity filariasis. The symptoms exhibited by individuals afflicted with filariasis are diverse and contingent upon several variables, including the species of parasite, the host’s health and immune response, and the stage of infection. While many classical parasitological techniques are considered indispensable tools for the diagnosis of parasitic infections in humans, alternative methods are being sought due to their limitations. Novel tests based on host–parasite interactions offer a rapid, simple, sensitive, and specific diagnostic tool in comparison to traditional parasitological methods. This article presents methods developed in the 21st century for the diagnosis of filariasis caused by invasion from W. bancrofti, B. malayi, O. volvulus, and O. lupi, as well as techniques that are currently in use. The development of modern diagnostic methods based on molecular biology constitutes a significant advancement in the fight against filariasis.

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Section: Next-generation Methods For Filariasis Diagnosticsmentioning

confidence: 99%

Beyond Tradition: Exploring Cutting-Edge Approaches for Accurate Diagnosis of Human Filariasis

Pietrzak,

Łuczak,

Wiśniewski

2024

Pathogens

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“…The most commonly targeted genetic markers, namely nuclear small subunit ribosomal RNA (rRNA), internal transcribed spacer (ITS) regions of nuclear ribosomal DNA (rDNA) and the mitochondrial (mt) cytochrome c oxidase subunit 1 (cox1) gene, have been used to study genetic variation in Gnathostoma [90][91][92][93]. PCR-coupled sequencing and bioinformatics methods have also been used to identify and differentiate Gnathostoma in fixed and paraffin-embedded tissues, and can be used for the reappraisal of individual cases [94].…”

Section: Diagnosismentioning

confidence: 99%

Human gnathostomiasis: a neglected food-borne zoonosis

Sun

Elsheikha

et al. 2020

Parasites Vectors

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Background Human gnathostomiasis is a food-borne zoonosis. Its etiological agents are the third-stage larvae of Gnathostoma spp. Human gnathostomiasis is often reported in developing countries, but it is also an emerging disease in developed countries in non-endemic areas. The recent surge in cases of human gnathostomiasis is mainly due to the increasing consumption of raw freshwater fish, amphibians, and reptiles. Methods This article reviews the literature on Gnathostoma spp. and the disease that these parasites cause in humans. We review the literature on the life cycle and pathogenesis of these parasites, the clinical features, epidemiology, diagnosis, treatment, control, and new molecular findings on human gnathostomiasis, and social-ecological factors related to the transmission of this disease. Conclusions The information presented provides an impetus for studying the parasite biology and host immunity. It is urgently needed to develop a quick and sensitive diagnosis and to develop an effective regimen for the management and control of human gnathostomiasis.

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