Rapid Differentiation of Influenza A Virus Subtypes and Genetic Screening for Virus Variants by High-Resolution Melting Analysis

Lin, Jih Hui; Tseng, Ching Ping; Chen, Yen–Ju; Lin, Chy Yung; Chang, Shy Shin; Wu, Ho Sheng; Cheng, Ju Chien

doi:10.1128/jcm.02015-07

Cited by 75 publications

(55 citation statements)

References 41 publications

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“…As long as reference samples are included in the HRMA for each independent analysis, the HRMA profile can distinguish variants with differences in Tm values as little as 0.15°C. 17 This can be achieved by the use of average of HRM GCPs. However, variations in Tm values and discrepancies in GCPs were found among the tested samples in the current study when using ARMS qRT-PCR combined with HRMA.…”

Section: Discussionmentioning

confidence: 99%

“…3 Since 2008, RT-PCR combined with the high-resolution melting (HRM) analysis (HRMA) has been used to detect several viruses, including Infectious bursal disease virus and Influenza A virus, and viruses within the genera Norovirus and Capripoxvirus. 8,17,28 The melting temperature (Tm) and the patterns of the HRM curves that are generated by denaturation of the various amplicons along a temperature gradient can be used to discriminate genetic variations in virus strains. 6 Accordingly, a RT-PCR assay combined with HRMA has also been reported for the Australian IBV wildtype and vaccine strains based on the hypervariable region of 3′-UTR.…”

Section: Introductionmentioning

confidence: 99%

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The genotyping of Infectious bronchitis virus in Taiwan by a multiplex amplification refractory mutation system reverse transcription polymerase chain reaction

Huang

Chan

et al. 2014

J VET Diagn Invest

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Abstract. Infectious bronchitis virus (IBV; Avian coronavirus) causes acute respiratory and reproductive and urogenital diseases in chickens. Following sequence alignment of IBV strains, a combination of selective primer sets was designed to individually amplify the IBV wild-type and vaccine strains using a multiplex amplification refractory mutation system reverse transcription polymerase chain reaction (ARMS RT-PCR) approach. This system was shown to discriminate the IBV wildtype and vaccine strains. Moreover, an ARMS real-time RT-PCR (ARMS qRT-PCR) was combined with a high-resolution analysis (HRMA) to establish a melt curve analysis program. The specificity of the ARMS RT-PCR and the ARMS qRT-PCR was verified using unrelated avian viruses. Different melting temperatures and distinct normalized and shifted melting curve patterns for the IBV Mass, IBV H120, IBV TW-I, and IBV TW-II strains were detected. The new assays were used on samples of lung and trachea as well as virus from allantoic fluid and cell culture. In addition to being able to detect the presence of IBV vaccine and wild-type strains by ARMS RT-PCR, the IBV Mass, IBV H120, IBV TW-I, and IBV TW-II strains were distinguished using ARMS qRT-PCR by their melting temperatures and by HRMA. These approaches have acceptable sensitivities and specificities and therefore should be able to serve as options when carrying out differential diagnosis of IBV in Taiwan and China.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The genotyping of Infectious bronchitis virus in Taiwan by a multiplex amplification refractory mutation system reverse transcription polymerase chain reaction

Huang

Chan

et al. 2014

J VET Diagn Invest

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“…40 . In the field of virology, Lin et al 41 developed high resolution melting analysis for subtype differentiation of the influenza A virus and for detection of newly emerging variants. The influenza B virus has also been studied using the melting curve analysis by Nakagawa et al 42 , who demonstrated that the assay not only detected its antigenic variants but also allowed for more precise analysis of the antigenic shifts in the influenza B virus.…”

Section: Melting Analysis For Genotyping Purposesmentioning

confidence: 99%

The Potential of High Resolution Melting Analysis (Hrma) to Streamline, Facilitate and Enrich Routine Diagnostics in Medical Microbiology

Ruskova¹,

Raclavsky²

2011

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub

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Background. Routine medical microbiology diagnostics relies on conventional cultivation followed by phenotypic techniques for identification of pathogenic bacteria and fungi. This is not only due to tradition and economy but also because it provides pure culture needed for antibiotic susceptibility testing. This review focuses on the potential of High Resolution Melting Analysis (HRMA) of double-stranded DNA for future routine medical microbiology.Methods and Results. Search of MEDLINE database for publications showing the advantages of HRMA in routine medical microbiology for identification, strain typing and further characterization of pathogenic bacteria and fungi in particular. The results show increasing numbers of newly-developed and more tailor-made assays in this field. For microbiologists unfamiliar with technical aspects of HRMA, we also provide insight into the technique from the perspective of microbial characterization.Conclusions. We can anticipate that the routine availability of HRMA in medical microbiology laboratories will provide a strong stimulus to this field. This is already envisioned by the growing number of medical microbiology applications published recently. The speed, power, convenience and cost effectiveness of this technology virtually predestine that it will advance genetic characterization of microbes and streamline, facilitate and enrich diagnostics in routine medical microbiology without interfering with the proven advantages of conventional cultivation.

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“…High resolution melting analysis (HRMA) of amplicons of fewer than 200 bp have been used for detection of single nucleotide polymorphisms, mutation scanning, and genotyping (Lin et al, 2008). The use of larger amplicons has been reported to reduce sensitivity and specificity of HRMA (Chou et al, 2005;Krypuy et al, 2006 The polymorphism of rabbit C-reactive protein promoter was investigated in two groups subjected to strict divergent selected rabbits according variation range of live-born kits at least after 3 litters.…”

Section: Introductionmentioning

confidence: 99%

The C-reactive protein promoter polymorphism of selected rabbits

Vašíček¹,

Ondruška²,

Vašíčková³

et al. 2016

Acta fytotechn zootechn

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Rapid Differentiation of Influenza A Virus Subtypes and Genetic Screening for Virus Variants by High-Resolution Melting Analysis

Abstract: We assessed the use of high-resolution melting (HRM) analysis for the rapid identification of influenza

Cited by 75 publications

References 41 publications

The genotyping of Infectious bronchitis virus in Taiwan by a multiplex amplification refractory mutation system reverse transcription polymerase chain reaction

The genotyping of Infectious bronchitis virus in Taiwan by a multiplex amplification refractory mutation system reverse transcription polymerase chain reaction

The Potential of High Resolution Melting Analysis (Hrma) to Streamline, Facilitate and Enrich Routine Diagnostics in Medical Microbiology

The C-reactive protein promoter polymorphism of selected rabbits

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