Rapid discrimination of different Apiaceae species based on HPTLC fingerprints and targeted flavonoids determination using multivariate image analysis

Shawky, Eman; Kheir, Rasha M. Abou El

doi:10.1002/pca.2749

Cited by 31 publications

(14 citation statements)

References 32 publications

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“…The Umbelliferae family (Apiaceae) is one of the largest taxa among higher plants with more than 300 genera and 3000 species that are indigenous to the Mediterranean basin and distributed in central Asia and Europe. 1 Owing to a myriad of sensory metabolites and phytochemical constituents, members of this family are well recognized for their use in traditional medicine, culinary use, beverages and in cosmetics. Apart from serving as spices, they have been the target of many research work for their health benets as anti-tumor, antimicrobial, anti-inammatory, digestive, antispasmodic, estrogenic, hypoglycemic, radical scavenging and hypolipidemic activities.…”

Section: Introductionmentioning

confidence: 99%

UHPLC-MS metabolome based classification of umbelliferous fruit taxa: a prospect for phyto-equivalency of its different accessions and in response to roasting

et al. 2020

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Section: Introductionmentioning

confidence: 99%

UHPLC-MS metabolome based classification of umbelliferous fruit taxa: a prospect for phyto-equivalency of its different accessions and in response to roasting

et al. 2020

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“…Principal component analysis and HCA are powerful chemometric methods that have been widely used for classification studies (Shawky & Abou El Kheir, 2018). PCA involves unsupervised pattern recognition, which is the most frequently used method for reducing the dimensionality of numerical datasets in a multivariate space.…”

Section: Discussionmentioning

confidence: 99%

Simultaneous determination of phenolic metabolites in Chinese citrus and grape cultivars

Chen

Hong

Dao-fu

et al. 2020

PeerJ

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Background As the major bioactive compounds in citrus and grape, it is significant to use the contents of flavonoids and phenolic acids as quality evaluation criteria to provide a better view of classifying the quality and understanding the potential health benefits of each fruit variety. Methods A total of 15 varieties of citrus and 12 varieties of grapes were collected from Fujian, China. High-performance liquid chromatography method was used for the simultaneous determination of 17 phenolic compounds, including gallic acid, chlorogenic acid, caffeic acid, syringic acid, ρ-coumaric acid, ferulic acid, benzoic acid, salicylic acid, catechin, epicatechin, resveratrol, rutin, naringin, hesperidin, quercetin, nobiletin and tangeritin in the peels of citrus and grape cultivars. Further, the cultivars of citrus and grape were classified using principal component analysis (PCA) and hierarchical cluster analysis (HCA). Results A thorough separation of the 17 compounds was achieved within 100 min. The tested method exhibited good linearity (the limits of detection and limits of quantification were in the range of 0.03–1.83 µg/mL and 0.09–5.55 µg/mL, respectively), precision (the relative standard deviations of repeatability were 1.02–1.97%), and recovery (92.2–102.82%) for all the compounds, which could be used for the simultaneous determination of phenolic compounds in citrus and grape. Hesperidin (12.93–26,160.98 µg/g DW) and salicylic acid (5.35–751.02 µg/g DW) were the main flavonoids and phenolic acids in 15 citrus varieties, respectively. Besides, the hesperidin (ND to 605.48 µg/g DW) and salicylic acid (ND to 1,461.79 µg/g DW) were found as the highest flavonoid and the most abundant phenolic acid in grapes, respectively. A total of 15 citrus and 12 grape samples were classified into two main groups by PCA and HCA with strong consistency.

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“…This family is very rich in terms of secondary metabolites. Coumarin [12,13], flavonoid [13][14][15], acetylenic compounds [13], sesquiterpene lactones [16][17][18] and essential oils [19][20][21][22][23] are obtained from many genera of the family.…”

Section: Introductionmentioning

confidence: 99%

Angelica Sylvestris Var. Sylvestris L.: Essential Oils and Antioxidant Activity Evaluation

Ağalar

Göğer

Demırcı

et al. 2020

Eskişehir Technical University Journal of Science and Technology a - Applied Sciences and Engineering

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In this study, the essential oils and antioxidant activities of A. sylvestris var. sylvestris belongs to the Apiaceae family were evaluated. The essential oils from dried roots, leaves, flowers and fruits obtained by hydrodistillation were analyzed by Gas Chromatography and Gas Chromatography-Mass Spectroscopy, simultaneously. The volatile compounds were characterized by using in-house and commercially libraries. The major compounds were spathulenol (12.4%), germacrene D (10.6%), humulene (7.6%) in the leave essential oil; elemol (5.4%), 10-epi--eudesmol (5.4%), spathulenol (4.8%) in the root oil; pinen (42.0, 23.2%) and -phellandrene (25.5, 9.1%) in the flower and the fruit essential oils, respectively. The possible antioxidant activities of each essential oil were determined at in vitro conditions. Any essential oil at 10 mg/ml concentration showed DPPH  scavenging activity while the fruit essential oil was found to be most active against ABTS  radical. But this effect was lower than gallic acid.

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Rapid discrimination of different Apiaceae species based on HPTLC fingerprints and targeted flavonoids determination using multivariate image analysis

Abstract: The use of chemometrics tools for evaluation of fingerprints reduced expense and analysis time. The proposed method can be adopted for routine discrimination and evaluation of the phytochemical variability in different Apiaceae species extracts.

Cited by 31 publications

References 32 publications

UHPLC-MS metabolome based classification of umbelliferous fruit taxa: a prospect for phyto-equivalency of its different accessions and in response to roasting

UHPLC-MS metabolome based classification of umbelliferous fruit taxa: a prospect for phyto-equivalency of its different accessions and in response to roasting

Simultaneous determination of phenolic metabolites in Chinese citrus and grape cultivars

Angelica Sylvestris Var. Sylvestris L.: Essential Oils and Antioxidant Activity Evaluation

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