Rapid electronic detection of probe-specific microRNAs using thin nanopore sensors

Wanunu, Meni; Dadosh, Tali; Ray, Vishva; Jin, Jingmin; McReynolds, Larry A.; Drndić, Marija

doi:10.1038/nnano.2010.202

Cited by 667 publications

(831 citation statements)

References 55 publications

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“…In the future this will facilitate the detection of very small and short polymers. 24 The increase in the current amplitude will be demonstrated showing an increase from about 50 pA for single DNA strand for an unmodified nanocapillary to over 300 pA for a shrunken nanocapillary. Other applications which will benefit from a smaller diameter of the nanocapillary include near-field scanning optical microscopy (NSOM), scanning electrochemical microscopy (SECM), or nanobilayer coated glass capillaries.…”

Section: T He Resistive Pulse Technique (Also Called Coulter Countermentioning

confidence: 89%

Controllable Shrinking and Shaping of Glass Nanocapillaries under Electron Irradiation

Steinbock

Radenović

2013

Nano Lett.

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The ability to reshape nanopores and observe their shrinkage under an electron microscope is a powerful and novel technique. It increases the sensitivity of the resistive pulse sensing and enables to detect very short and small molecules. However, this has not yet been shown for glass nanocapillaries. In contrast to their solid-state nanopore counterparts, nanocapillaries are cheap, easily fabricated and in the production do not necessitate clean room facilities. We show for the first time that quartz nanocapillaries can be shrunken under a scanning electron microscope beam. Since the shrinking is caused by the thermal heating of the electrons, increasing the beam current increases the shrink rate. Higher acceleration voltage on the contrary increases the electron penetration depth and reduces the electron density causing slower shrinkage. This allows us to fine control the shrink rate and to stop the shrinking process at any desired diameter. We show that a shrunken nanocapillary detects DNA translocation with six times higher signal amplitudes than an unmodified nanocapillary. This will open a new path to detect small and short molecules such as proteins or RNA with nanocapillaries.

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Section: T He Resistive Pulse Technique (Also Called Coulter Countermentioning

confidence: 89%

Controllable Shrinking and Shaping of Glass Nanocapillaries under Electron Irradiation

Steinbock

Radenović

2013

Nano Lett.

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“…Thus, solid-state nanopores made in a 7 nm thick SiN membrane with a smallest constriction of 3 nm (Fig. 4) were shown to enable in ideal conditions discrimination of dsRNA even from dsDNA and transfer RNA (tRNA) [42]. Figure 4.…”

Section: Nanopore-based Resistive Pulse Sensing Of Micrornasmentioning

confidence: 99%

“…A separation and preconcentration step based on magnetic beads modified with dsRNA-selective p19 protein was used to separate the target miRNAs from cellular RNA. [42] Reprinted by permission from Macmillan Publishers Ltd: Nature Nanotechnology, Ref. [42], copyright (2010).…”

Section: Nanopore-based Resistive Pulse Sensing Of Micrornasmentioning

confidence: 99%

Electrochemical Detection of miRNAs

Lautner

Gyurcsányi

2014

Electroanalysis

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The recent progress made in the development of electrochemical methods for microRNA (miRNA) detection is presented. This progress is conceived to be largely due to the invention of novel assay methodologies and the use of various bioreagents and nanostructures. These enable a rigorous control over the sensing interface, provide enormous signal amplifications and single-base mismatch specificity. Femtomolar or even subfemtomolar detection limits were shown to be feasible by electrochemical assays. Thus electrochemical detection methodologies are of perspective for diagnostic miRNA detection.

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“…There are a great number of excellent fabrication methods for miRNA biosensors with high sensitivity. The micro‐ring resonator,54 the nanowire‐based,55, 56 the nanopore‐based,57, 58 and the polymer‐based barcode assay59 have already been demonstrated. This nanotechnology‐based miRNA sensors show atto‐ or zepto‐mole level detection providing great sensing potential for cancer patients.…”

Section: Introductionmentioning

confidence: 99%

Contact Transfer Printing of Side Edge Prefunctionalized Nanoplasmonic Arrays for Flexible microRNA Biosensor

et al. 2015

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For a nanoplasmonic approach of wearable biochip platform, understanding correlation between near‐field enhancement on nanostructures and sensing capability is a crucial step to improve the sensitivity in biosensing. A novel and effective method is demonstrated to increase sensitivity with the enhanced electric fields and to reduce noise with targeted functionalization enabled by transferring side edge prefunctionalized (SEPF) nanostructure arrays onto flexible substrates. Nanostructure sidewalls have selective biochemically functional terminals for the hybridization of microRNAs (miRNAs) and the immobilization of resonant nanoparticles, thus forming hetero assemblies of the nanostructure and the nanoparticles. The unique configuration has shown ultrasensitive biosensing of miRNA‐21 in a 10 × 10−15 m level by a red‐shift in scattering spectra induced by a plasmon coupling. This ultrasensitive SEPF nanostructure arrays are fabricated on a flexible substrate using a contact transfer printing with a release layer of trichloro(1H, 1H, 2H, 2H‐perfluorooctyl)silane. The introduction of the release layer at a prefunctionalizing step has proven to provide selective functionalization only on the sidewalls of the nanostructures. This reduces a background noise caused by the scattering from nonspecifically bound nanoparticles on the substrate, thus enabling reliable and precise detection.

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Rapid electronic detection of probe-specific microRNAs using thin nanopore sensors

Cited by 667 publications

References 55 publications

Controllable Shrinking and Shaping of Glass Nanocapillaries under Electron Irradiation

Controllable Shrinking and Shaping of Glass Nanocapillaries under Electron Irradiation

Electrochemical Detection of miRNAs

Contact Transfer Printing of Side Edge Prefunctionalized Nanoplasmonic Arrays for Flexible microRNA Biosensor

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