Rapid extraction, isolation, and quantification of oleanolic acid from<i>Lantana camara</i>L. Roots using microwave and HPLC-PDA techniques

Verma, Subash Chandra; Jain, Chahat; Nigam, Sukriti; Padhi, Madan Mohan

doi:10.1556/achrom.25.2013.1.12

Cited by 22 publications

(9 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The obtained thermogram exhibited a single endothermic peak at 309 °C. More recently, several authors have reported OA melting points in the range 306–313 °C [ 1 , 4 ]. The OA boiling temperature has been established at 553–554 °C at 760 mmHg ( , accessed on 18 November 2021).…”

Section: Oleanolic Acid Characterizationmentioning

confidence: 99%

Oleanolic Acid: Extraction, Characterization and Biological Activity

2022

View full text Add to dashboard Cite

Oleanolic acid, a pentacyclic triterpenoid ubiquitously present in the plant kingdom, is receiving outstanding attention from the scientific community due to its biological activity against multiple diseases. Oleanolic acid is endowed with a wide range of biological activities with therapeutic potential by means of complex and multifactorial mechanisms. There is evidence suggesting that oleanolic acid might be effective against dyslipidemia, diabetes and metabolic syndrome, through enhancing insulin response, preserving the functionality and survival of β-cells and protecting against diabetes complications. In addition, several other functions have been proposed, including antiviral, anti-HIV, antibacterial, antifungal, anticarcinogenic, anti-inflammatory, hepatoprotective, gastroprotective, hypolipidemic and anti-atherosclerotic activities, as well as interfering in several stages of the development of different types of cancer; however, due to its hydrophobic nature, oleanolic acid is almost insoluble in water, which has led to a number of approaches to enhance its biopharmaceutical properties. In this scenario, the present review aimed to summarize the current knowledge and the research progress made in the last years on the extraction and characterization of oleanolic acid and its biological activities and the underlying mechanisms of action.

show abstract

Section: Oleanolic Acid Characterizationmentioning

confidence: 99%

Oleanolic Acid: Extraction, Characterization and Biological Activity

2022

View full text Add to dashboard Cite

show abstract

“…Analytical equipment such as thin layer chromatography (TLC) [ 21 ], High Performance Liquid Chromatography (HPLC) [ 22 , 27 ] and nuclear magnetic resonance (NMR) [ 29 ] are common platforms that have been used in the characterization and identification of OA. Isolation of OA usually involves defatting of the pulverized powder of the plant with a non-polar solvent such as n -hexane [ 30 ] or petroleum ether [ 26 ]. This is typically followed by preparation of the crude extracts, and the isolation of oleanolic acid using techniques such as precipitation and crystallization [ 21 ], vacuum liquid chromatography [ 29 ] and column chromatography [ 31 ].…”

Section: Introductionmentioning

confidence: 99%

Oleanolic Acid and Its Derivatives: Biological Activities and Therapeutic Potential in Chronic Diseases

2017

View full text Add to dashboard Cite

The increasing demand for natural products as an alternative therapy for chronic diseases has encouraged research into the pharmacological importance of bioactive compounds from plants. Recently, there has been a surge of interest in the therapeutic potential of oleanolic acid (OA) in the prevention and management of chronic diseases. Oleanolic acid is a pentacyclic triterpenoid widely found in plants, including fruits and vegetables with different techniques and chromatography platforms being employed in its extraction and isolation. Several studies have demonstrated the potential therapeutic effects of OA on different diseases and their symptoms. Furthermore, oleanolic acid also serves as a framework for the development of novel semi-synthetic triterpenoids that could prove vital in finding therapeutic modalities for various ailments. There are recent advances in the design and synthesis of chemical derivatives of OA to enhance its solubility, bioavailability and potency. Some of these derivatives have also been therapeutic candidates in a number of clinical trials. This review consolidates and expands on recent reports on the biological effects of oleanolic acid from different plant sources and its synthetic derivatives as well as their mechanisms of action in in vitro and in vivo study models. This review suggests that oleanolic acid and its derivatives are important candidates in the search for alternative therapy in the treatment and management of chronic diseases.

show abstract

“…Recent examples are reported below. HPLC (or reversed-phase RP-HPLC) with various detector modes (UV, evaporative light scattering detection, photodiode array detection) appeared as the most employed technique for quantitation of UA and/or OA in plant extracts from Lantana camara [19], Salvia chinensis [20], Thymus ssp [21], and Nyctanthes arbor-tristis [22]. Otherwise, co-quantitation of OA and UA was achieved by LC-MS in oregano ( Origanum spp.)…”

Section: Introductionmentioning

confidence: 99%

Identification and Quantitation of Ursolic and Oleanolic Acids in Ilex aquifolium L. Leaf Extracts Using 13C and 1H-NMR Spectroscopy

2019

View full text Add to dashboard Cite

Leaves of Ilex aquifolium L. have been used for their therapeutic properties. In previous studies, components contained in the leaves were first isolated by various chromatographic techniques. Then, quantitation of oleanolic and ursolic acids, which are responsible for the biological and therapeutic activities of the plant, was performed by HPLC, HPTLC, and somewhat by GC-MS. Our objective was to develop a simple method that allows the identification of compounds contained in the leaves of Corsican I. aquifolium and to quantify ursolic and oleanolic acids. Leaves were successively extracted with hexane and dichloromethane. The extracts were chromatographed on silica gel and the fractions of column chromatography submitted to 13C-NMR analysis, following a computerized method developed in the laboratory. 13C-NMR allowed the identification of various triterpenes including ursolic acid and oleanolic acid. Quantitation of both acids was achieved, for the first time, by 1H-NMR after validation of the method (accuracy, precision, linearity, limit of detection and limit of quantitation). Ursolic and oleanolic acids accounted for 55.3% and 20.8% of the dichloromethane extract, respectively. This represents 1.3% and 0.5% of the mass of dried leaves. 1H-NMR spectroscopy appeared as a powerful tool for a rapid quantitation of biologically active compounds from I. aquifolium.

show abstract

Rapid extraction, isolation, and quantification of oleanolic acid fromLantana camaraL. Roots using microwave and HPLC-PDA techniques

Cited by 22 publications

References 20 publications

Oleanolic Acid: Extraction, Characterization and Biological Activity

Oleanolic Acid: Extraction, Characterization and Biological Activity

Oleanolic Acid and Its Derivatives: Biological Activities and Therapeutic Potential in Chronic Diseases

Identification and Quantitation of Ursolic and Oleanolic Acids in Ilex aquifolium L. Leaf Extracts Using 13C and 1H-NMR Spectroscopy

Contact Info

Product

Resources

About