Rapid extraction of high‐quality genomic DNA from Porphyra yezoensis (Bangiales, Rhodophyta)

Abstract

SUMMARY We developed a simple, rapid and stable method for extraction of high molecular weight DNA from the marine red alga Porphyra yezoensis Ueda using both guanidium treatment and QIAGEN™ kit (Funakoshi, Tokyo, Japan). The method does not require expensive equipment and complex steps. The DNA yield averaged 1.5 μg 100 mg−1 of Porphyra tissue and the A260/A280 and A230/A260 ratios of the DNA were approximately 1.8 and 0.4, respectively. It was of sufficient quality to be used for not only polymerase chain reactions but also other DNA manipulation techniques such as restriction digestion and construction of genomic libraries.

SIMPLE METHOD FOR RNA PREPARATION FROM CYANOBACTERIA1

“…However, all these methods are either too time consuming or expensive to use for RNA preparations from a large number of strains. In addition, traditional methods for nucleic acid extraction from cyanobacteria require large amounts of cells due to low yields (Nakajima et al 2000). Accordingly, this study presents a more rapid, simple, and economical method for the preparation of RNA from various cyanobacteria when compared with previously reported methods.…”