Rapid fabrication of homogeneously distributed hyper-branched gold nanostructured electrode based electrochemical immunosensor for detection of protein biomarkers

Bakshi, Shrishty; Mehta, Samriddhi; Kumeria, Tushar; Shiddiky, Muhammad J. A.; Popat, Amirali; Choudhury, Sangeeta; Bose, Sudeep; Nayak, Ranu

doi:10.1016/j.snb.2020.128803

Cited by 31 publications

(15 citation statements)

References 36 publications

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“…7 Our demonstration of SAM-based PCB sensors will likely be applicable to many other SAM-based sensors. 23,30,53,64 Our prototype clearly demonstrates substantial superiority in terms of cost and portability over existing alternatives. We think that this open-source NAAT prototype fabricated with mass-produced PCBs and ubiquitous electronics will have an impact on addressing current commercial bottlenecks in POC diagnostic tests.…”

Section: Discussionmentioning

confidence: 90%

Portable electroanalytical nucleic acid amplification tests using printed circuit boards and open-source electronics

Filella

Ainla

Khaliliazar

et al. 2022

Analyst

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Section: Discussionmentioning

confidence: 90%

Portable electroanalytical nucleic acid amplification tests using printed circuit boards and open-source electronics

Filella

Ainla

Khaliliazar

et al. 2022

Analyst

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“…166 In a comparative analysis, LSV was found to be superior to chronoamperometric electrochemical deposition for a nanostructured gold electrodes. 74 Gold electrode surface generated using LSV showed better surface homogeneity, higher surface-to-volume ratio, and better redox activity. The electrochemical deposition method is a simple, cost-effective, and time-saving method, but it needs a conductive surface.…”

Section: Metal Particlesmentioning

confidence: 89%

“…187 A prototype for the detection of epithelial growth factor receptor (EGFR), based on the formation of SAM was proposed. 74 Here mercaptoundecanoic acid (MUA) was used to construct the monolayer. The −SH group of MUA binds to the Au surface via a Au−S bond, and the exposed terminal −COOH group was used for EDC-NHS coupling for immobilization of the EGFR antibody.…”

Section: Electrospray Deposition (Esd)mentioning

confidence: 99%

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Advances in Bioelectrode Design for Developing Electrochemical Biosensors

Kalita,

Gogoi,

Minteer

et al. 2023

ACS Meas. Sci. Au

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The critical performance factors such as selectivity, sensitivity, operational and storage stability, and response time of electrochemical biosensors are governed mainly by the function of their key component, the bioelectrode. Suitable design and fabrication strategies of the bioelectrode interface are essential for realizing the requisite performance of the biosensors for their practical utility. A multifaceted attempt to achieve this goal is visible from the vast literature exploring effective strategies for preparing, immobilizing, and stabilizing biorecognition elements on the electrode surface and efficient transduction of biochemical signals into electrical ones (i.e., current, voltage, and impedance) through the bioelectrode interface with the aid of advanced materials and techniques. The commercial success of biosensors in modern society is also increasingly influenced by their size (and hence portability), multiplexing capability, and coupling in the interface of the wireless communication technology, which facilitates quick data transfer and linked decision-making processes in real-time in different areas such as healthcare, agriculture, food, and environmental applications. Therefore, fabrication of the bioelectrode involves careful selection and control of several parameters, including biorecognition elements, electrode materials, shape and size of the electrode, detection principles, and various fabrication strategies, including microscale and printing technologies. This review discusses recent trends in bioelectrode designs and fabrications for developing electrochemical biosensors. The discussions have been delineated into the types of biorecognition elements and their immobilization strategies, signal transduction approaches, commonly used advanced materials for electrode fabrication and techniques for fabricating the bioelectrodes, and device integration with modern electronic communication technology for developing electrochemical biosensors of commercial interest.

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“…With the high price of bulk gold, the implementation of nanogold has created an economical solution for sensing material fabrication. Importantly, gold nanostructures have provided a remarkable surface area to volume ratio, which apparently reinforces signal acquisition and reduces the content of the materials required for the same surface area [12,13].…”

Section: Introductionmentioning

confidence: 99%

Gold Nanoframe Array Electrode for Straightforward Detection of Hydrogen Peroxide

et al. 2021

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The nanostructuring of a sensing membrane is performed through colloidal nanosphere lithography (NSL) techniques with a tiny polystyrene nanobead template 100 nm in size. The solvent ratio adjustment has been proven to be effective in assisting the monolayer deposition of small templating particles with minimal defects. Two distinct structures, namely, a billowy gold nanostructure (BGN) where the nanobead template is left unetched and a gold nanoframe array (GNA) with a regular ring-like structure after template removal, are used for the extended-gate field-effect transistor (EGFET) electrodes. The GNA structure generates an electroactive surface area significantly (~20%) larger than its geometrical area as well as a greater surface roughness than the BGN. When integrated with the portable constant voltage–constant current (CVCC) FET circuitry for pH screening to determine the optimized measurement conditions for H2O2 sensing, the GNA sensing membrane also shows more improved Nernstian sensitivity at ~50 mV/pH than the BGN electrode. The more optimized sensitivity is then proven using the GNA in the detection of H2O2, the most common representative reactive oxygen species (ROS) involved in the environment, food, and neurodegenerative diseases, such as Parkinson´s and Alzheimer´s diseases. The GNA electrode has a sensitivity of 70.42 mV/log µM [H2O2] and a limit of detection (LoD) of 1.183 µM H2O2. The integrated ion sensing system employing unique, highly ordered gold array gate electrodes and a portable CVCC circuit system has shown a stable real-time output voltage signal, representing an alternative to bulky conventional FET devices for potential on-site H2O2 detection.

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Rapid fabrication of homogeneously distributed hyper-branched gold nanostructured electrode based electrochemical immunosensor for detection of protein biomarkers

Cited by 31 publications

References 36 publications

Portable electroanalytical nucleic acid amplification tests using printed circuit boards and open-source electronics

Portable electroanalytical nucleic acid amplification tests using printed circuit boards and open-source electronics

Advances in Bioelectrode Design for Developing Electrochemical Biosensors

Gold Nanoframe Array Electrode for Straightforward Detection of Hydrogen Peroxide

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