Rapid fluorescence method for screening Salmonella spp. from enteric differential agars

Aguirre, Paula; Cacho, J.; Folgueira, Lola; López, Marisela; Garcia, José Antônio Dias; Velasco, A C

doi:10.1128/jcm.28.1.148-149.1990

Cited by 28 publications

(11 citation statements)

References 5 publications

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“…The PPV was also the highest with NBGL (61%), although if growth in SS or HE was combined with the C 8 -esterase test, the PPVs of these media rose to 100%. The usefulness of this test has already been established (1,10,17,18). The excellent performance of NBGL coincides with the results of Poisson and colleagues (11,13), which gave average sensitivities and PPVs higher than those of SS and HE.…”

Section: Discussionsupporting

confidence: 79%

“…This substrate interacts with the Salmonella C 8 -esterase, leading to the release of umbelliferone, which is strongly fluorescent at 365 nm. A total of 5 l of the reagent was added to two to three suspect colonies, and the test was considered positive if a blue fluorescence of the colony was observed under a Wood lamp within 5 min (1,10,17,18). The cytochrome oxidase test was obtained from Difco.…”

Section: Methodsmentioning

confidence: 99%

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Comparison of five plating media for isolation of Salmonella species from human stools

et al. 1996

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A comparative study was carried out to evaluate the performances of different culture media for the recovery of Salmonella spp. from 1,000 routine samples of human stools. By direct plating we tested Salmonella-Shigella agar (SS), Hektoen enteric agar (HE), bismuth sulfite agar (BS), novobiocin-brilliant green-glycerol-lactose agar (NBGL) and SM-ID medium (SM), and after selenite enrichment, we tested all of the media except HE. C 8-esterase and oxidase tests were used for the screening of Salmonella spp. on SS and HE. The total number of Salmonella isolates from direct culture was 74, with respective sensitivities and positive predictive values (PPVs) of 78.4 and 61%, 64.9 and 18.7%, 36.5 and 34.2%, 55.4 and 20.7%, and 39.2 and 43.9% for NBGL, SS, HE, BS, and SMID, respectively. After enrichment, the total number of Salmonella isolates was 88. The respective sensitivities and PPVs obtained were 90.9 and 62.5%, 92 and 17%, 90.9 and 32% and 93.2 and 71.3% for NBGL, SS, BS, and SM, respectively. According to our results, NBGL in direct plating was the medium with the highest sensitivity with respect to the sensitivities of the other media, with significant statistical differences (P < 0.05). Likewise, the PPV for NBGL was also the highest (61%). After enrichment in selenite broth, the sensitivities of the four media tested were similar, with the best PPV obtained with SM (71.3%); this was followed by NBGL (62.5%). When C 8-esterase was used on SS or HE, the PPVs improved from less than 40% to about 100%.

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Section: Discussionsupporting

confidence: 79%

Section: Methodsmentioning

confidence: 99%

Comparison of five plating media for isolation of Salmonella species from human stools

et al. 1996

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“…The MUCAP test was found to be very sensitive, rapid and easy to perform but not very specific, due to the high number of false-positive results. This is in accordance with the findings of other researchers (Humbert et al 1989;Aguirre et a l . 1990;Betts and McGoldrick 1990).…”

Section: Resultssupporting

confidence: 94%

“…1990;Betts and McGoldrick 1990). The combination of the MUCAP test and selective media gives higher rates of specificity (Hurnbert et al 1989;Aguirre et al 1990). Since the most false-positive strains are cytochrome oxidase positive, the combination of MUCAP and cytochrorne oxidase test is recommended (Ruiz et al 1991).…”

Section: Resultsmentioning

confidence: 99%

Comparison of three rapid methods for identification of Salmonella spp

Manafi

Willinger

1994

Lett Appl Microbiol

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A study was carried out to compare three rapid methods for detection of Salmonella spp. The fluorogenic MUCAP test (Biolife, Italy), the SM‐ID agar test (bioMérieux, France) and the Rambach agar test (Merck, Germany) were used in this study to examine 103 strains (69 Salmonella strains and 34 non‐Salmonella strains). Two conventional culture media, Hektoen and Leifson agars, were also included. The sensitivities of the MUCAP, SM‐ID, Rambach and Hektoen agar tests for pure strains were 100, 93, 88 and 99%, respectively, and their specificities were 74, 97, 76 and 59%, respectively. A total of 100 stool samples from patients with acute diarrhoea was also tested and showed great discrepancy between the different methods. In agreement with other investigators, it was found that the discriminating capacity of Rambach and SM‐ID as primary plating media was very restricted. The MUCAP test was very sensitive, rapid and easy to perform but not very specific. In view of these results, it is essential to combine different methods for the accurate and reliable detection of Salmonella strains.

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“…No positive reaction occurred when 63 1-galnon-Salmonella members of the family Enterobacteriaceae were grown on Rambach agar. Conversely, other investigators (1) found 5 and 12% false-positive strains among the lactose-negative bacteria when they used Mac-Conkey and SS agars, respectively. However, we do not know which caprilate solvent they used.…”

mentioning

confidence: 85%

Detection of salmonellae by using Rambach agar and by a C8 esterase spot test

Freydière

Gille

1991

J Clin Microbiol

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In food microbiology, Rambach agar facilitates the differentiation of non-typhi Salmonella through a specific red pigmentation of the colonies. The usefulness of Rambach agar was examined relative to its usefulness to the field of clinical microbiology. Of 170 non-typhi SalmoneUa strains, 92 and 97% gave bright red colonies after 24 and 48 h of incubation, respectively, while 100% of 112 other members of the family Enterobacteriaceae were of a different color (blue, green, beige, or colorless). Red colonies were also found with five of five Acinetobacter isolates and one of three Pseudomonas isolates. To further detect Salmonella typhi and the rare beige or colorless colonies atypical of Salmonella isolates, a C8 esterase detection spot test was carried out. With UV light, that test revealed fluorescent colonies for all Salmonella isolates tested.

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Rapid fluorescence method for screening Salmonella spp. from enteric differential agars

Cited by 28 publications

References 5 publications

Comparison of five plating media for isolation of Salmonella species from human stools

Comparison of five plating media for isolation of Salmonella species from human stools

Comparison of three rapid methods for identification of Salmonella spp

Detection of salmonellae by using Rambach agar and by a C8 esterase spot test

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